Use LEFT and RIGHT arrow keys to navigate between flashcards;
Use UP and DOWN arrow keys to flip the card;
H to show hint;
A reads text to speech;
31 Cards in this Set
- Front
- Back
- 3rd side (hint)
|
Calibration
Review We calibrate an instrument to relate instrument measurement signal to _____________ |
analyte concentration |
|
|
|
Calibration
We calibrate an instrument to relate ____________ to analyte concentrate |
instrument signal |
|
|
|
Calibration
What is the upper end? |
The highest value we can reliably report without sample manipulation. |
The _________ _____ we can ____ ______ without ____ ___. |
|
|
Calibration
What is the upper end? |
The highest value we can reliably report without sample manipulation. |
The ________ we can ___________ without ________. |
|
|
Calibration
WHAT IS THE UPPER END? |
The highest value we can reliably report without sample manipulation
What happens if a result exceeds upper end? |
Dilution |
|
|
Calibration
Upper end
What happens if a result exceeds the upper end? |
Dilution
What is the low end? |
Lowest value we can reliably report |
|
|
Calibration
Low end
Def. |
Lowest value we can reliably report. |
______ _____ we can ____ _____. |
|
|
Calibration
Low end |
Lowest value we can reliably report.
This is not necessary _____. What must be assessed? |
zero LoB, D, Q |
|
|
Calibration
Low end Lowest value we can reliably report.
This is not ________ zero. We must ______LoB, __, ____. |
necessarily assess D Q |
|
|
|
Calibration
Low end
This is not necessarily _____. We must assess ___________ |
zero LoB,D, Q
Def of low end |
Lowest value we can reliably report |
|
|
Define LoB |
It represents the 95th percentile of analytical noise for the assay. |
It _____ the ____ ______ of ____ ____ for the ____. |
|
|
Define LoB |
It _______ the __________ of __________ for the _____. |
represents 95th percentile analytical noise assay |
|
|
DEFINE LOB |
It represents the 95th percentile of analytical noise for the assay.
Formula |
LoB=mean of the blank + 1.645 (SD of blank) |
|
|
Formula for LoB |
LoB=______ of the blank + 1.645 (___ of blank) |
mean SD |
|
|
Formula for LoB Test |
LoB= Mean of the blank +1.645 (SD of blank)
Why 1.645 is used? |
z-score of one-sided tail at 5% |
|
|
LoB
Def. Formula |
It represents the 95th percentile of analytical noise for the assay.
LoB=Mean of the blank+1.645 (SD of blank)
1.645 is used because it is the ___-score of ____-sided tail at _____. |
z one 5% |
|
|
LoD
Def. |
The lowest concentration of an analyte that can be distinguished from LoB/0 |
The _____ ______ of an _____ that can be _____ from ____/___ |
|
|
LoD
Def. |
The _________________ of an _____ that can be ______ from ________. |
Lowest concentration analyte distinguisdhed LoB/0 |
|
|
LoD
Def. |
The lowest concentration of an analyte that can be distinguished from LoB/0
if the analyte is really present LoD___LoB |
> |
|
|
LoD
Notes |
____ of samples at the LoD will fal below the LoB by design and chance distribution. |
5% see slide 34 in lecture 8 for more |
|
|
LoD
Formula |
LoD=LoB+1.645(SD low concentration sample) |
|
|
|
LoD LoD=____+1.645(SD _____________) |
____=LoB+______ (____ low concentration sample) |
LoD 1.645 SD |
|
|
LOD
Formula |
LoD=LoB+1.645(SD low concentration sample) |
|
|
|
LoQ ____ LoD |
> |
|
|
|
LoQ canot be ___ LoD, but the two could be ______. |
< equal |
|
|
|
LoQ
Formula |
Total analytical error=bias+(2*SD) |
_________________=_____+ (__*___) |
|
|
LoQ
Formula Total ______ error = ______ + (2*____) |
analytical bias SD |
|
|
|
LoQ Formula
_____ analytical ______= _______+ (___*______) |
Total error bias 2 SD |
|
|
|
LoQ
Formula |
Total analytical error=Bias + 2*SD |
|
|
|
LoQ
Notes |
The lowest concentration at which a test analyte can be reliably measured based on total analytical error
2nd def. |
Imprecision of 20% CV or better at low analyte level |
|
|
Notes |
See Lecture 8 slide 25-45 |
|
