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16 Cards in this Set
- Front
- Back
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Not known to consistenly cause disease in healthy individules(agents)
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BSL 1 agents
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Standard microbiological pratices(practices)
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BSL 1 pratices
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None required (safety equipment)
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BSL1 safety
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Facilities include laboratory bench sink required
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BSL1 facilities
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Agent associated with human diesease
Routes of transmission through percutaneous injury, ingestion, mucous membrane exposure |
BSL 2 agent
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BSL-1 practice plus:
limited access biohazard warning signs "sharps" precaution Biosafety manual defining and needed waste decontamination or medical surveillance policies |
BSL 2 practice
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Primary barriers:
Class I or II BSCs or other physical containment devices used for all manipulation of agents that cause splashes or aerosis of infectious materials (PPE) laboratory coats,gloves, face protection as needed |
BSL 2 safety equipment
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BSL-1 plus: Autoclave
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BSL 2 facility
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Indigenous of exotic agents with potential for aerosol transmission
Disease may have serious of lethal consequences |
BSL 3 agent
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BSL-2 practice plus:
controlled lav access decontamination of all wase decontamination of all lab clothing before laundering |
BSL 3 practice
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Primary barriers:
class I or II BSCs or other physical containment devices used for all open manipulations of agent PPE: protective laboratory clothing, gloves,respiratory protection as needed |
BSL 3 safety equipment
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BSL-2 plus:
physical separation from access corridors self-closing ,double-door access exhausted air not recirculated negative airflow into laboratory |
BSL 3 facility
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Dangerous/exotic agents that pose high rish of life-threatening disease
aerosol=transmitted lab infections have occurred;or related agent with unknown rish of transmission |
BSL 4 agent
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BSL-3 practice plus:
clothing change before entering shower before exit all material decontaminated on exit from facility |
BSL 4 practice
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Primary barriers:
all procedures conducted in class III BSCs or class I or II BSCs in combination with full-body, air supplied,positive pressure personnel suit. |
BSL 4 safety equipment
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BSL-3 plus:
separate building or isolated zone dedicated supply and exhaust, vacuum, and decontamination systems other requirements outlined in the text |
BSL 4 facility
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