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77 Cards in this Set
- Front
- Back
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Which of the following protective equipment must be worn (by law) when one is handling formaldehyde?
a) impervious apron b) lab coat c) scrub suit d) white uniform |
a) impervious apron
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The short-term exposure limit (STEL) for formaldehyde set by the Formaldehyde Standard is:
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2.0ppm
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Which of the following may be disposed of in the sanitary sewer system according to Environmental Protection Agency (EPA) and Centers for Disease Control (CDC) guidelines?
a) absolute alcohol b) silver salts c) pulverized tissue d) blood |
d) blood
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500mL of a .55% solution of potassium metabisulfate must be prepared. How many grams of potassium metabisulfate should be used?
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2.75gm
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Which of the following hazard classifications is of primary concern with picric acid?
a) mechanical b) biological c) chemical d) fire/explosion |
d) fire/explosion
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The Formaldehyde Standard sets the time-weighted average (TWA) for formaldehyde at:
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.75ppm
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A temperature of 4⁰C is commonly associated with the laboratory:
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refrigerator
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Potassium dichromate falls into which of the following hazard classifications?
a) mechanical b) biological c) chemical d) fire/explosion |
c) chemical
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Which of the following pHs is considered basic?
a) 6.0 b) 6.5 c) 7.0 d) 7.5 |
d) 7.5
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A microscope with 2 eyepieces is:
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binocular
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When used in a National Fire Protection Association (NFPA) diamond, which of the following numbers is indicative of the most severe hazard?
a) 1 b) 2 c) 3 d) 4 |
d) 4
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Flammability hazards are indicated in which section (color) of the NFPA diamond?
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red
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According to the EPA, it is illegal to dispose of concentrated acetic acid in the sanitary sewer system because it is classified as:
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corrosive
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How many mL of water should be added to 80mL of concentrated formaldehyde to obtain a solution of 10% formalin?
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720mL
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During microscopic examination, if the section remains in focus when changing objectives, the objectives are:
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achromatic
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Paraffin sections of brain are cut at 15 micrometers. This is equivalent to how many millimeters?
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.015
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A .5% solution of light green is needed. The bottle contains only 4.25gm of dye powder. How many mL of solution can be prepared if all of the dye is used?
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850mL
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The handling of biohazards is governed by the ____ standard.
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blood-borne pathogen standard
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A solution is prepared that contains 5gm of NaCl in 200mL water. This is equivalent to which of the following percent solutions?
a) .4 b) 1.0 c) 2.5 d) 5.0 |
c) 2.5
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A dye solution that is applied after the primary stain has acted is called a(n):
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counterstain
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HCl acid may be disposed of in the sink, provided it is:
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neutralized to a pH between 3 and 11
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If a reagent is biodegradable, the disposal is always:
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determined by water solubility
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To obtain a total magnification of 1000 with an ocular of 10x magnification, the objective must have a magnification of:
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100x
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Fires involving wood and paper are considered:
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Class A Fires
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During microscopic review of an H&E-stained section, it is noted that there is insufficient light on the slide. The best way to correct this is by:
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opening the iris diaphragm
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Organic solvents should disposed of by:
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collecting them in waste containers for future disposal or distillation
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500mL of 3% alcian blue in 1% acetic acid contains how much alcian blue? acetic acid? water?
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15g alcian blue
5mL acetic acid 495mL water |
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Another way of expressing 350mg is as (how many grams):
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.35g
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Following staining with a solution prepared from a new bottle of eosin dye powder, the color results are not the same as those obtained with the previously used eosin dye powder. The best course of action is to:
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determine whether both dyes have the same dye content
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Components of living tissue do not require staining if they are to be examined by (type of microscope):
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phase contrast microscopy
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An acidic or basic substance added to a solution to prevent a change in pH is called a:
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buffer
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The "high-dry" objective usually has a magnification of:
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45x
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A section 5 micrometers thick would be equivalent to how many millimeters in thickness?
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.005mm
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Individual virus particles cannot be seen by light microscopy, but can be visualized by:
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electron microscopy
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How much 95% alcohol must be used to prepare 1000mL of 70% alcohol?
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737mL
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Nontoxic, basic stains that are suspended in sterile water and injected into living animals are referred to as:
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vital
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To prepare 50mL of 2% ferric chloride from a 10% solution, how many mL of the 10% solution will be required?
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10
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Doubly refractive particles are also called:
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birefringent
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Tissue can be fixed in the microwave oven because it causes:
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rapid molecular movement
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How many mL of water should be added to 100mL of a 20% solution of silver nitrate to obtain a 10% solution?
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100
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To prepare 500mL of .55% potassium metabisulfite solution, how many grams of potassium metabisulfite would be required?
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2.75
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A reagent labeled with NFPA diamond has a 4 in the blue quadrant. This means that:
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the hazard is extreme and exposure could result in death
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Before adjusting the pH of the Warthin-Starry solution, the pH meter should be calibrated with a buffer solution of pH:
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4.0
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One cubic centimeter is equal to how many milliliters?
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1mL
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Some striated muscle cells are as much as 400mm in length. This is equivalent to how many centimeters?
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40
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The ability of a microscope objective to separate small detail is called the ____ power.
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resolving
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A fire involving hydrocarbon reagents is classified as a:
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Class B fire
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Which of the following reactions describes the process of reducing silver to its visible metallic state without the aid of extraneous agents?
a) argentaffin b) decidual c) argyrophilic d) leutinizing |
a) argentaffin
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The primary purpose of the Biological Stain Commission is to certify that dye:
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meets all qualities required by the Commission
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A temperature of 37⁰C is most likely associated with:
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the laboratory incubator
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A gradual increase in staining time, with worsening overstaining, is noted when using a microwave oven equipped with a temperature probe. The first step in correcting this problem would be to:
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clean the temperature probe
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How many mL of 29% ferric chloride stock solution should be used when preparing 50mL of 2% ferric chloride?
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3.45
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Which of the following is the formula for calculating a percent (w/v) solution?
a) (grams of solute x 100)/(volume of solvent) b) (grams of solute) x (volume of solvent) x 100 c) (volume of solvent x 100)/(grams of solute) d) (grams of solute) x (volume of solvent)/100 |
a) (grams of solute x 100)/volume of solvent
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How many mL of 10% sodium hydroxide are required to prepare 200mL of a 4% solution?
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80
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Solvent recovery systems are able to separate contaminants from solvents because the contaminants:
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differ in boiling points from teh solvents
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During a microwave Grocott staining procedure, the methenamine-silver reagent boils. In response to this, one should:
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decrease the heating time of the reagent
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When fractional distillation solvent recovery systems are used, xylene:
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will be of higher purity recycled than when original
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What volume of a stock solution of 25% NaCl would be required to prepare 250mL of a 5% solution?
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50mL
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To help prevent back strain, the hips should form an angle of ___ degrees between truck and thighs when a technician is seated at the microtome.
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60 to 90
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Polarizing microscopes use a polarizer and a(n):
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analyzer
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A researcher asks that sections of his tissue be cut at 70 micrometers. This is equivalent to how many millimeters in thickness?
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.07
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A solution contains 20g of solute dissolved in .5L of water. What is the percentage of this solution?
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4%
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How many milliliters of a 3% solution can be made if 6gm of solute are used?
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200mL
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When a solution containing HCl acid is to be discarded in the sink, it should be:
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neutralized between pH 3 and pH 11
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A small electrical fire occurs in the laboratory. Which of the following substances would be appropriate for use in extinguishing the fire?
a) water b) soda acid c) carbon tetrachloride d) carbon dioxide |
carbon dioxide
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Microscopic review of slides stained for the presence of microorganisms requires the use of immersion oil. The purpose of the oil is to:
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improve the resolution
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Microwaves are produced by a(n):
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magnetron
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One micron is equal to:
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.000001
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A chemical that should be used under a hood because of the possibility of extreme respiratory irritation is:
a) picric acid b) diaminobenzidine c) hydrochloric acid d) chromic acid |
c) hydrochloric acid
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When using the microwave oven at an intermediate power setting, the technician notices that the oven light dims intermittently, accompanied by variations in sound levels. The most appropriate course of action is to:
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do nothing, the equipment is functioning normally
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How many grams of aniline blue should be used to prepare 750mL of a 5% solution?
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37.5
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A stock solution of Harris Hematoxylin is being prepared for use in the routine H&E procedure. In order to link the dye to the tissue, which of the following must be added to the solution?
a) mercuric oxide b) aluminum ammonium sulfate c) ferric chloride d) acetic acid |
b) aluminum ammonium sulfate
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An employee has been complaining of tingling or numbness in the hands, an aching neck, and shoulder and wrist pain. Referral to the health clinic would most likely reveal the employee most likely has:
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chronic repetitive drama
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To meet most inspection criteria, the laboratory is expected to prepare procedure manuals to conform to the guidelines published by the:
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National Committee for Clinical Laboratory Standards (NCCLS)
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The function of the condenser on the light microscope is to:
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concentrate light on the tissue specimen
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The possibility of repetitive motion injury (RMI) may be reduced by:
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utilizing an automated machine when available
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When an inorganic substance is dissolved in water, the usual result is:
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ionization
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