Bmb 462 Exam 3 **capa** Recombinant Dna Libraries

Front 1

Is the production and use of recombinant DNA libraries is true or false.

E. coli is frequently used to store and amplify libraries made using plasmid vectors.

Back 1

True:

Front 2

Is the production and use of recombinant DNA libraries is true or false.

Nitrocellulose paper is used during library screening to create a replica of the bacterial colonies on a plate.

Back 2

True:

Front 3

Is the production and use of recombinant DNA libraries is true or false.

With some plasmids, antibiotic selection can determine which bacterial colonies contain plasmids with inserts.

Back 3

True:

Front 4

Is the production and use of recombinant DNA libraries is true or false.

Antibiotic selection is frequently used to select for bacterial cells that contain plasmids following electroportation.

Back 4

True:

Front 5

Is the production and use of recombinant DNA libraries is true or false.

In the context of screening a genomic library, a probe is complimentary to the sequence of the DNA being sought.

Back 5

True:

Front 6

Is the production and use of recombinant DNA libraries is true or false.

A cDNA library would only contain sequences from genes that were actively transcribed in the tissue from which it was made.

Back 6

True:

Front 7

Is the production and use of recombinant DNA libraries is true or false.

DNA ligase is used to join DNA fragments following cleavage with restriction endonucleases.

Back 7

True:

Front 8

Is the production and use of recombinant DNA libraries is true or false.

Nitrocellulose paper is used during library screening to immobilize DNA on a solid support and allow hybridization.

Back 8

True:

Front 9

Is the production and use of recombinant DNA libraries is true or false.

Different types of cloning vectors allow cloning of different sized DNA fragments

Back 9

True:

Front 10

Is the production and use of recombinant DNA libraries is true or false.

Restriction endonucleases are used to produce a cDNA from mRNA.

Back 10

False:

Front 11

Is the production and use of recombinant DNA libraries is true or false.

A cDNA library would contain promoter sequences from genes that were actively transcribed in the tissue from which it was made.

Back 11

False:

Front 12

Is the production and use of recombinant DNA libraries is true or false.

All types of cloning vectors are suitable for cloning DNA fragments between 35 Kbp and 45 Kbp in length.

Back 12

False:

Front 13

Is the production and use of recombinant DNA libraries is true or false.

Following ligation of a recombinant plasmid it can be introduced into E. coli by restriction endonucleases.

Back 13

False:

Front 14

Is the production and use of recombinant DNA libraries is true or false.

In the context of screening a genomic library, a probe is an oligonucleotide that is used to determine which bacterial colonies contain recombinant DNA plasmids.

Back 14

False:

Front 15

Is the production and use of recombinant DNA libraries is true or false.

All types of cloning vectors require the use of E. coli to store and amplify the recombinant DNA.

Back 15

False:

Front 16

Is the production and use of recombinant DNA libraries is true or false.

DNA ligases act as molecular scissors to cut DNA at specific locations.

Back 16

False:

Front 17

Is the production and use of recombinant DNA libraries is true or false.

Reverse transcriptase is used to join DNA fragments following cleavage with restriction endonucleases.

Back 17

False:

Front 18

Is the production and use of recombinant DNA libraries is true or false.

All types of cloning vectors require the use of E. coli to store and amplify the recombinant DNA.

Back 18

False:

Front 19

Is the production and use of recombinant DNA libraries is true or false.

A genomic library would only contain sequences from genes that were actively transcribed in the tissue from which it was made.

Back 19

False:

Front 20

Is the production and use of recombinant DNA libraries is true or false.

In the context of screening a genomic library, antibiotic selection allows researchers to determine which bacterial colony contains the DNA sequence of interest.

Back 20

False: