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### 20 Cards in this Set

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 1 meter is _____ in metric system is equals to: 10 decimeters=100cm=1000mm 1 micrometer is = 0.000001m (10 -6 m) 1 nm = 0.000000001 m or 10 -9 m 1 Angstrom = 10 -10 m or 0.1 nm 1 mm = 0.001 m = 10 -3 m 1 micrometer is = 0.000001 m = 10 -6m 1 nanometer is = 0.000000001 m = 10 -9 m 1 picometer = 0.000000000001 m = 10 -12m What is a Light Microscope? it refers to the use of any kind of microscope that uses visible light to observe microorganisms Types of Light Microscope Compound light microscope - commonly used in micro; uses visible light; bright-field illumination is used for stained smears. How is the total magnification of a specimen calculated in a light microscope? by multiplying the objective lens magnification power by the ocular lens magnification What are the parts of a microscope and how is the specimen examined? The magnification is achieved when light rays from the illuminator (light source) pass thru a condenser (has lenses that direct the light thru the specimen. Then light rays pass into the objective lens (the lens closest to the specimen). The image is magnified again by the ocular lens. What is Resolution? Specifically, it refers to the ability of the lenses to distinguish two points a specified distance apart. What is a Refractive Index? It is a measure of the light- bending ability of a medium. We changed the RI of specimens by staining them. What is Bright-Field Illumination? The field of vision in a compound microscope is brightly illuminated. By focusing the light, the condenser produces a bright-field illumination. What is the maximum resolution, or resolving power of a compound light microscope (LM)? 0.2 micrometer. Maximum magnification is 2000X. Why are specimens stained? Specimens are stained to increase the difference between the refractive indexes of the specimen and the medium. What is the purpose of using immersion oil? Immersion oil is used with the oil immersion lens to reduce light loss between the slide & the lens. How are unstained cells processed? Unstained cells are more productively observed using darkfield, phase contrast, or DIC microscopy. What is the path of light in a compound light microscope? From the Illuminator, to the condenser lens thru the specimen to the objective lens to the ocular lens then ends to the line of vision.