Blood Bank

Front 1

First recorded blood transfusion

Back 1

Pope innocent VIi

Front 2

Discovery of circulatory system

Back 2

William Harvey

Front 3

Animal to animal transfusion

Back 3

Richard Lower

Hint 3

Dog to dog

Front 4

Animal to human transfusion

Back 4

Jean Baptist Denis

Hint 4

Sheep to human

Front 5

First successful human blood transfusion

Back 5

James Blundle

Hint 5

Performed on a woman suffering from postpartum hemorrhage

Front 6

First to work on blood transfusion and blood preservation techniques

Back 6

Dr Charles Drew

Hint 6

Director of first American red cross blood bank at Presbyterian hospital

Front 7

Sodium Phosphate as anticoagulant

Back 7

Braxton Hicks

Front 8

Sodium Citrate

Back 8

Albert Hustin

Front 9

Determined minimum non-toxic amount of citrate

Back 9

Richard Lewison

Front 10

Citrate Dextose

Back 10

Rous and Turner

Hint 10

1916

Front 11

Acid citrate dextrose

Back 11

Loutit and Mollison

Hint 11

1943

Front 12

Utilized glycerol to extend RBC life by 10 years

Back 12

Audrey smith

Hint 12

1950

Front 13

Citrate phosphate dextrose (CPD) as standard preservative

Back 13

Gibson

Hint 13

1957

Front 14

Vein to vein transfusion

Back 14

Edward Lindemman

Hint 14

1913

Front 15

Syringe valve apparatus

Back 15

Lester Unger

Hint 15

1915

Front 16

ABO group discovered by

Back 16

Karl Landsteiner

Hint 16

1901

Front 17

Defined 4th blood group :AB

Back 17

Alfred Von decastello

Adriano sturlii

Hint 17

1902

Front 18

MN and P Discovered

Back 18

Karl Landsteiner

Philip Levine

Front 19

RH Discovered

Back 19

Karl Landsteiner

Alexander wiener

Hint 19

1940

Front 20

World first blood bank in chicago

Back 20

Cook county Hospital

Hint 20

Leadership of Dr. Bernard Fantus in 1937

Front 21

First community based blood center

Back 21

Irwin memorial blood bank

Hint 21

1941

Front 22

ISBT 001

Back 22

ABO blood group system

Front 23

Leading cause of death in hemolytic transfusion reaction fatalities

Back 23

Transfusion of wrong ABO group

Hint 23

Severe intravascular hemolysis

Front 24

In 2009, it was the frequent cause of death

Back 24

Transfusion related acute lung injury

Hint 24

TRALI

Front 25

Genes codes for specific glycosyl transferase that add sugar to a precursor substance

Back 25

Paragloboside and glycan

Hint 25

Determines blood group

Front 26

Amorphic blood type, does NOT code for any enzyme

Back 26

O group

Hint 26

Absence of A and B antigen

Front 27

810,000 to 1,170,000 antigen sites

Back 27

Group A

Front 28

610,000 to 830,000 antigen

Back 28

B group

Front 29

B enzyme compete more efficiently for the H substance than A enzyme

Back 29

A+B gene

Hint 29

A - 600,000

B - 720,000

Front 30

Described the theory for the inheritance of the ABO group

Back 30

Bernstein

Hint 30

1924

Front 31

ABO gene is

Back 31

Autosomal(somatic chromosome)

Front 32

A group and B group genes are ?

Back 32

Co-dominant

Front 33

A blood group and B blood group are ?

Back 33

Dominant

Front 34

Considered amorph

Back 34

O gene

Front 35

Group O phenotype

Back 35

Autosomal recessive

Front 36

Two identical allel

Back 36

Homozygous

Front 37

Produced by the genotype to control blood group antigen

Back 37

Phenotype

Front 38

Gene needed for ABO to be expressed

Back 38

H gene

Front 39

Gene needed to be expressed in secretion

Back 39

Se gene

Front 40

Basic precursor material for formation of ABH antigens

Back 40

Paragloboside or glycan

Front 41

ABO antigens forms as early as

Back 41

37th day of fetal life

Front 42

Full expression of ABO gene in what age?

Back 42

2-4 years old

Front 43

ABO antigen on RBC are synthesized on type ? And give it's beta # linkage

Back 43

Type 2, 1-4

Front 44

ABO antigen on SECRETION are synthesized on type ? And give it's beta # linkage

Back 44

Type 1, 1-3

Front 45

ABH substance can be found in DUBSTAMP give each one

Back 45

Digestive juices

Urine

Bile

Saliva

Tears

Ammiotic fluid

Milk

Pleural fluid

Front 46

Excessive ABH substance in secretion can be observed in ???

Back 46

PIC

Pseudomocinus ovarian cyst

Intestinal obstruction

Carcinoma of pancreas and stomach

Front 47

Assay to determine secretor status

Back 47

Heme agglutination inhibition assay

Hint 47

DUMPSTAMP

Front 48

Forms of antigen

Back 48

Unbranched straight complex

H1 H2

Branched complex

H3 H4

Front 49

Reactivity to anti-H greatest to least

Back 49

O>A²>B>A²B>A¹>A¹B

Front 50

Subgroup? anti-A¹ and anti A, anti AB

Back 50

A¹ subgroup

Front 51

Subgroup? Anti A, and anti AB

Back 51

A²

Front 52

MF reaction with Anti A, anti AB

Back 52

A3

Front 53

Reacts w/ anti AB, no reaction to anti A

Back 53

Ax

Front 54

Removal of antibody from serum

Back 54

Adsorption

Front 55

Removal of antibody from surface of RBC

Back 55

Elution

Front 56

Contains A1 and A antigen

Back 56

A1

Front 57

Contains A antigen only

Back 57

A2

Front 58

Bm is converted to B if incubated with

Back 58

uracil diphosphate

Front 59

in bombay phenotype Autosomal recessive is caused by mutation in?

Back 59

FUT 1 gene and FUT 2

Front 60

FUT 1 gene

Back 60

silenced H gene

Front 61

FUT gene

Back 61

silenced Se gene

Front 62

T/F

Bombay phenotype can donate to any blood group but can only be transfused with blood from another bombay?

Back 62

True

Front 63

Trace / Absent amount of A,B and H antigen on RBC with normal expression in secretions and bodily fluids

Back 63

Para Bombay

Front 64

Para-bombay is caused by

Back 64

Mutation in FUT 1 gene

Silence FUT 1 gene with an active FUT 2 gene

Front 65

ABO antibodies react at what temp?

Back 65

RT(20-24)

Front 66

Anti A and Anti B are what immunoglobulin? how about Anti-A,B

Back 66

IgM

IgG

Front 67

Lectin of Anti-A1

Back 67

Dolichos biflorus

Front 68

Lectin of Anti-B

Back 68

Bandeiraea simplicifolia

Front 69

Lectin of Anti-H

Back 69

Ulex europaeus

Front 70

Lectin of Anti-N

Back 70

Vicia gramiNea

Front 71

Lectin of Anti-M

Back 71

Iberes aMara

Front 72

Anti-T, Th

Back 72

Arachis hypogaea

Front 73

Anti-Tn

Back 73

Salcia sclarea

Front 74

Type of antisera used in forward typing

Back 74

Monoclonal antibodies

Front 75

Anti sera A color

Back 75

Tryphan blue

Front 76

Anti sera B color

Back 76

Acriflavin yellow

Front 77

Ensures the specificity of antisera in QC

Back 77

Negative control

Front 78

ensures the reactivity of anti sera

Back 78

positive control

Front 79

T/F

THE POSITIVE CONTROL SHOULD BE STRONGLY REACTIVE

Back 79

FALSE

Front 80

Universal RBC donor

Back 80

O

Front 81

Universal Plasma donor

Back 81

AB

Front 82

Universal RBC recipient

Back 82

AB

Front 83

Universal Plasma recipient

Back 83

O

Front 84

Grading

one solid agglutinate

Back 84

4+

Front 85

Roleaux pseudoagglutination can cause false (+) or False (-)

Back 85

False (+)

Front 86

Acquired A phenomenon, give the organism that causes this

Back 86

P. mirabilis

Front 87

Acquired B phenomenon, give the organisms/disease that causes this

Back 87

E. coli
P. vulgaris
Intestinal obstruction
Carcinoma of colon/stomach
C. tettani

Hint 87

EPICC

Front 88

Acquired B cells will not react with pH

Back 88

<6 or >8.5

Front 89

This is treated to RBC to reacetylate surface molecule and thus decreasing reactivity with Anti-B

Back 89

Acetic anhydride

Front 90

This detects true B in resolution of acquired B phenomenon

Back 90

Monoclonal Anti-B

Front 91

Detects Pseudo B by not reacting in resolution of acquired phenomenon

Back 91

Acidified Anti-B

Front 92

In emergency situations, this blood bag group and unit is issued

Back 92

Group O rh(-) Prbc

Front 93

Discrepancies problem in reverse typing only

Back 93

Group 1

Front 94

Discrepancies problem in forward typing only

Back 94

Group 2

Front 95

Leukemias and Hodgkin's disease are diseases that causes what type of discrepancies

Back 95

Group 2

Front 96

Agammaglobulinemia, Malignant lymphoma, immunosupressive drugs are diseases that causes what type of discrepancies

Back 96

Group 2

Front 97

Newborn and elderly age,what type of discrepancies do they belong

Back 97

Group 2

Front 98

Enzyme treatment in which RBC's are pretreated w/ enzyme and retested w/ an anti sera what group of discrepancies is this resolution

Back 98

Group 2

Front 99

Protein and plasma problem in discrepancies

Back 99

Group 3

Front 100

Multiple myeloma, Waldenstroms macroglobulinemia are diseases that causes what type of discrepancies

Back 100

Group 3

Front 101

Resolution in Group 3 discrepancies

Back 101

Saline Replacement techniques

Front 102

Cold reactive autoantibodies, Unexpected ABO isoagglutinins, what group of discrepancies

Back 102

Group 4

Front 103

RHD and RHCE is proposed by

Back 103

Tippet Patricia

Front 104

RHD and RHCE are located on chromosome

Back 104

chrosome 1

Front 105

RHAG located in chrosome

Back 105

chrosome 6

Front 106

The first 3 letters in RH represent the

Back 106

system/collection/series

Front 107

The last 3 letters represent the

Back 107

antigenic specificity

Front 108

Rh phenotype that is caused by abnormalities in Transmembrane protein

Back 108

Rh null

Front 109

Rh phenotype that is caused by an allele carryying RHD is in trans position to the allele that is carrying C

Back 109

C in trans / Positional D

Front 110

Rh phenotype that is caused by weak expression of D

Back 110

weak D

Front 111

Rh phenotype that is caused by 1 or more epitope within D protein is missing/altered

Back 111

Partial D

Front 112

A compound antigen; expressed 'c' and 'e' are in cis position

Back 112

F antigen

Front 113

Warm reacting antibodies and react best at 37

Back 113

IgG

Front 114

Strong D expression because of no Cc/Ee expression

Back 114

Exalted D

Front 115

All IgG can fix/bind complement except

Back 115

IgG4

Front 116

IgG that cannot cross the placenta

Back 116

IgG2

Front 117

HTR due to Rh incompatibilities usually result

Back 117

Extravascular hemolysis

Front 118

Exposure to less than ____ can stimulate antibody production in an Rh(-) person

Back 118

0.1 ml

Front 119

Warm autoantibodies often appear to have

Back 119

anti-e like specificity

Front 120

LW blood group system antigen is carried by

Back 120

ICAM 4

Front 121

Was originally thought to be the same as the Rh family; now recognized as distinct from

Back 121

D antigen

Front 122

To differentiate D Ag from LW, treat cells with

Back 122

DTT

Front 123

Check cells are

Back 123

RBC coated with IgG antibodies

Rh (+) coated with anti-D antibody

Front 124

Inadequate washing in weak D typing can cause false?

Back 124

False Negative due to neutralization of AHG

Front 125

THE ONLY MAJOR SYSTEM NOT PRODUCED BY RBCS

Back 125

LEWIS SYSTEM

Front 126

FUT 3 is called

Back 126

Lewis gene

Front 127

Le antigens in secretion are

Back 127

glycoproteins

Front 128

Le cell bound antigens absorbed onto RBC's from plasma are

Back 128

glycolipids

Front 129

Lewis antigen at birth

Back 129

Le(a-b-)

Front 130

Lewis antigen after 10 days

Back 130

Le(a+b-) to Le(a+b+)

Front 131

Lewis antigen after 6 years

Back 131

Le(a-b+)

Front 132

Lewis antigen non-secretor after 10 days

Back 132

Le(a+b-)

Front 133

Le(a-b+) / Leb is associated with

Back 133

H. pylori

Front 134

On glycophorin A
MN -differ in amino acids at positions

Back 134

1 & 5

Hint 134

M: __serine, __glycine
N: __ Leucine, ___glutamic acid

Front 135

On glycophorin B
Ss differ in amino acid position

Back 135

29

Hint 135

Methoinine
theonine

Front 136

Formed when individual is S+s+

Back 136

U phenotype

Front 137

MN antibody that is enhanced at 6.5(acidic) and seen in multiparous women

Back 137

Anti M

Front 138

MN antibody that is enhanced at alkaline ph and seen in renal dialysis patients

Back 138

Anti-N

Front 139

MNS system is associated with GPA, a receptor for

Back 139

E.coli

Front 140

MNS system is associated with GPA and GPB, a receptor for

Back 140

P. falciparum

Front 141

Anti-P1 is neutralized by

Back 141

Hydatid cyst from E. granulosus

Front 142

This antibody is found in patients with fascioliasis

Back 142

Anti-P1

Front 143

She suffered from adenocarcinoma of the stomach and Anti-Pp1pk was described on her serum

Back 143

Mrs. Jay

Front 144

Produced in patients with Paroxymal cold hemoglobinuria

Back 144

Autoanti-P or Donath landsteiner

Front 145

____ receptor for Parvovirus B19

Back 145

P antigen

Front 146

_____ receptor for Shiga toxin which cause HUS

Back 146

pK antigen

Front 147

receptors for P-fimbriated uropathogenic E.coli

Back 147

P system antigens

Front 148

This disease is associated with individuals whose i antigen didn't convert to I antigen

Back 148

HEMPAS

Front 149

Associated with Primary atypical pneumoniae/walking pneumoniae

Back 149

Anti-I / Pathogenic Autoanti-I

Front 150

Associated with infectious mononucleosis by EBV, also acoholic cirrhosis

Back 150

Anti-i

Front 151

First blood group to be discovered after antiglobulin testing

Back 151

Kell system

Front 152

Come from the precursor substance ___ on RBC

Back 152

Kx

Front 153

Infection from S. faecium

Back 153

Acquired K

Front 154

Associated with E. coli O215 B15 infection

Back 154

Anti-K

Front 155

Absence of Kx on WBC

Back 155

CGD| Chronic granulomatous disease

Front 156

Absence of Kx on RBC

Back 156

Mcleod phenotype

Front 157

Duffy phenotype on white

Back 157

Fy(a+b+)

Fy(a-b+)

Front 158

Duffy phenotype on blacks

Back 158

Fy(a-b-)

Front 159

Duffy phenotype on Asians

Back 159

Fy(a+b-)

Front 160

Duffy blood group system is associated with resistance to

Back 160

P. vivax and P. knowlesi

Front 161

Duffy antigen that is related to susceptibility of P. vivax but not P.knowlesi

Back 161

Fy6

Front 162

Notorious in the blood bank, can cause

Back 162

delayed severe and fatal HTR

Front 163

Lutheran anti-Lu9 is found in patient with

Back 163

LE

Front 164

Anti Di^a and anti Di^b have caused HTR and HDFN and finding of characteristics of rbc's are

Back 164

Acanthocyte|spheroctye|Ovalocyte

Front 165

Expressed on RBC adhesion protein called ERMAP

Front 166

____ in northern europeans is 1% but is higher in mennonite population

Back 166

SC2

Front 167

Colton system antigens are located on an integral membrane protein called

Back 167

Aqua porin 1

Front 168

Antigens are not intrinsic to RBC membrane and FOUND IN C4 COMLEMENT

Back 168

Chido/rodgers system

Front 169

Chido/rodgers antibodies are neutralized by ??

Back 169

Plasma

Front 170

Knops system antigens are located on

Back 170

CR1|CD35 receptor

Front 171

Knops system antigens are decreased in

Back 171

SLE and CAD

Front 172

The RAPH system only antigen is

Back 172

MER-2

Front 173

Gill system antigen is found on the glycerol transporter ____

Back 173

aquaphorin 3

Front 174

Sd^a soluble antigen form is

Back 174

Tam-horsefall protein

Front 175

Anti-Sd^a is neutralized by ???

Back 175

urine

Front 176

Human leukocyte antigens AKA ____

Back 176

Bennett goodspeed antigens

Front 177

Bg a

Back 177

HLA B7

Front 178

Bg b

Back 178

HLA B17

Front 179

Bg c

Back 179

A28

Front 180

IgM antibodies

Back 180

Lewis

I

P1

M

ABH

N

Hint 180

LIPMĀN

Front 181

IgG antibodies

Back 181

DCEce

SsU

Fy

Kell

Jk

Lub

Front 182

Mixed field antibodies reaction

Back 182

Lutheran

Sda

Front 183

Absent in PNH III

Back 183

JMH

Cromer

Cartwright

Dombrock

Hint 183

JC cartrock

Front 184

Well developed at birth

Back 184

Duffy

MNSs

Kell

Kids

Hint 184

Doflamingo, Keller, Kidd, and MNS can kill baby - well developed at birth

Front 185

In vitro hemolysis antibodies

Back 185

Anti Le a,/Anti Le b

Anti Tka

Anti Vel

Front 186

Antibodies that have dosage effect

Back 186

RH

Duffy

MNS

Kids

Lutheran

Hint 186

High dosage red horse gi inom ni doflamingo, ki, Luther and MNS

Front 187

Decreased antibody in pregnancy

Back 187

Lewis

Front 188

Antibodies destroyed by enzymes

Back 188

Doffy, MNS, Xg^a

Minor: JMH, Chigo/roger, Pr

Front 189

Enhanced by enzymes

Back 189

Kidd
ABO
Lewis
I
Pi
R

Front 190

Hydatid cyst fluid, turtledove's egg whites, pigeon dropping can neutralize

Back 190

Anti-Pi

Front 191

Serum/Plasma can neutralize antibody ____

Back 191

Anti-Lewis

Front 192

Serum/Plasma (w/ complement) can neutralize____

Back 192

Anti-chido/roger

Front 193

Guinea Pig urine can neutralize

Back 193

Anti-Sd^a

Front 194

Human breast milk can neutralize ______

Back 194

Anti-I

Front 195

Increase of antibody than antigen

Back 195

PROZONE

Front 196

Increase of antigen than antibody

Back 196

POSTZONE

Front 197

Monoclonal AHG is produced through hybridoma technology using ____

Back 197

MICE

Front 198

Polyclonal AHG is produced through conventional method using ___

Back 198

Rabbit/Sheep/Goat

Front 199

In factors affecting AHG testing the serum cell ratio of Minimum ratio for routine tests is achieved by

Back 199

2 drops of serum and 1 drop of 5% rcs

Front 200

In factors affecting AHG testing the serum cell ratio of Minimum ratio for detecting weak antibodies is achieved by

Back 200

4 drops of serum and 1 drop of 3% rcs

Front 201

This potentiators allows antibody coated cells to come into closer contact with each other, reducing zeta potential, incubation time is 30-60 minutes

Back 201

Albumin(22%)

Front 202

Potentiators that enhance antibody uptake and decrease incubation time to 10-15 minutes

Back 202

LiSS (0.2% NaCl)

Front 203

Potentiator that increase antibody uptake by removing water molecules surrounding rbcs. Not for patients with high protein serum

Back 203

PEG

Front 204

RCF for AHG testing

Back 204

1000 rcf /20 sec

1500 rcf/1 min

Front 205

Specimen for AHG testing

Back 205

EDTA

Front 206

It often serves as the replacement to minor crossmatching

Back 206

Antibody screening

Front 207

Allows easier detection of underlying igG alloantibodies

Back 207

Use for Sulfhydryl agents or Dithiothreitol (DTT)

Front 208

Prevents complement activation in antibody screens

Back 208

Complement

Front 209

Done to remove rouleaux or prevent it

Back 209

Saline replacement

Front 210

Aids in identification of HTLA antibodies

Back 210

Antibody Titer determination

Front 211

Used to measure the production of IgG antibodies during pregnancy

Back 211

Antibody titer determination

Front 212

Create RBCs negative for all antigens of the Kell blood group system

Back 212

AET/DDT

Front 213

Destroy disulfide bonds on IgM, does not affect ___

Back 213

IgG

Front 214

ZZAP comprised of

Back 214

cysteine -activated papain (ficin) +DDT

Front 215

Removes antibodies attached to RBCS

Back 215

ZZAP

Front 216

Dissociates IgG from patient cells with DAT (+)

Back 216

Chloroquine diphosphate

Front 217

Only ____ should be sued as the AHG reagent as complement proteins are not dissociated with chloroquine

Back 217

Anti-IgG

Front 218

Removal of antibody from surfaces of RBC

Back 218

Elution

Front 219

Washed pRBC are treated with subsequent rapid freezing and thawing to cause lysis of the red blood cells, resulting in the freeing of cell bound antibodies

Back 219

Lui freeze method

Front 220

tubes in major crossmatching

Back 220

Donors red cell

Patient serum

Front 221

tubes in minor crossmatching

Back 221

Patient red cell

donors serum

Front 222

Age limit of those who can donate

Back 222

18 and above but below 60

Front 223

60 years old and above can donate if

Back 223

there's discretion of the BB physician

Front 224

Refers to removal and storage of blood from a donor-patient before an elective procedure for reinfustion during or after the procedure

Back 224

Pre-operative collection

Front 225

Blood is collected after administration of anesthesia and before a surgical procedure.

Back 225

Acute normovolemic hemodilution

Front 226

Collection and reinfusion of blood lost by a patient during the surgery

Back 226

intraoperative collection

Front 227

Most commonly used anticoagulant in aoheresis

Back 227

ACD/ ACD- A formula

Front 228

75% of plateletpheresis products should contain a minimum of

Back 228

3.0X10 platelets

Front 229

Used to collect immune plasma from donors with increased concentrations of certain plasma immunoglobulin for immunocompremised patients

Back 229

Plasmapheresis

Front 230

For patients who are severely neutropenic and unresponsive to antibiotics

Back 230

Leukapheresis

Front 231

Positive test for HBsAg

Back 231

Indefinite deferral

Front 232

men to men sexual contact since 1977

Back 232

permanent deferral

Front 233

Prostitution

Back 233

permanent deferral

Front 234

Positive Hepatitis C

Back 234

permanent deferral

Front 235

Received human derived growth hormone

Back 235

permanent deferral

Front 236

Have leukemia

Back 236

Indefinite deferral

Front 237

Deferral: received bovine insulin

Back 237

indefinitely deferral

Front 238

Imprisonment for 72 hours

Back 238

1 year deferral

Front 239

History of syphilis or Gonorrhea

Back 239

1 year deferral

Front 240

Tattoo, skin piercing, dental

Back 240

1 year deferral

Front 241

Tooth extraction

Back 241

3 days

Front 242

Malaria confirmed diagnosis

Back 242

3 years after asymptomatic

Front 243

Travel to endemic area (prior resident of endemic country <3 years after leaving)

Back 243

3 years after asymptomatic

Front 244

Travel to endemic area (prior resident of endemic country >3 years after leaving)

Back 244

1 year if asymptomatic

Front 245

Tegison

Back 245

Permanent deferral

Front 246

Soriatane

Back 246

3 years deferral

Front 247

Aspirin

Back 247

Plt: 3 days

WB: no deferral

Front 248

Rabies vaccine
HepaB Immune globulin
Gamma globulin
Rh immune globulin for HDN prevention

Back 248

12 months

Front 249

Rubella and Varicella zoster virus

Back 249

4 weeks

Front 250

Measles (robeola)
Oral polio
mumps

Back 250

2 weeks

Front 251

Cholera
Anthrax
Influenza

Back 251

No deferral

Front 252

Fainting and vasovagal reactions management *donor should respond by coughing

Back 252

Administer aromatic spirit of ammonia

Front 253

Ratio of ACD|CPD

Back 253

9:1

Front 254

If patient is allergic to iodine, use ____ for antiseptic

Back 254

chlorhexidine gluconate

Front 255

Apply tourniquet or BP cuffs

Back 255

1.5 inch above the site,| 40-60 mm/hg

Front 256

If collection exceeds 8 minutes; unit may not be suitable for _____

Back 256

Platelet

Front 257

Blood must be processed within____ hours of collection

Back 257

6-8 hours

Front 258

Most common disease transmitted by blood transfustion

Back 258

Hepatitis C

Front 259

______ is used for screening and _____ for confirmatory for blood bag testing for HIV

Back 259

ELISA
WESTERN BLOT

Front 260

Causative agent of adult T cell leukemia

Back 260

HTLV I

Front 261

New confirmatory test for HIV

Back 261

rHIVda

Front 262

Platelets are the only unit that is capable of transmitting _____ because this organism cannot live in blood stored for 3-4 days at 1-6

Back 262

Syphillis

Front 263

_________ serve as the confirmatory test for syphillis

Back 263

FTA-ABS

Front 264

Purpose is to maintain pH during storage

Back 264

Phosphate

Front 265

Improve viability of RBC for 21-35 days

Back 265

adenine

Front 266

decrease HCT from 70-80% to 50-60%

Back 266

Additive solutions

Front 267

HCT should not exceed

Back 267

80%

Front 268

Additive solutions composed of

Back 268

Saline
Adenine
Glucose
Mannitol

Hint 268

SAGM

Front 269

Component of additive solution that acts as a stabilizing agent

Back 269

Mannitol

Front 270

________ are solutions that used to restore ATP and 2,3-DPG levels.

Back 270

Rejuvenating solutions

Front 271

Rejuvenating solutions are composed of

Back 271

Phosphate
Inosine
Pyruvate
Adenine

Hint 271

PIPA

Front 272

RBC's can be rejuvenated at up to _____ after outdate

Back 272

3 days

Front 273

Rejuvenating solutions are done by placing RBC unit w/ ____ ml RS, for ____ hour at ____ C

Back 273

50 ml
1 hour
37C

Front 274

Purpose of rejuvenating solution

Back 274

Saving O type and rare blood

Front 275

molecules that enter the cell to prevent dehydration

Back 275

cryoprotective agents

Front 276

Example of penetrating cryoprotective agents

Back 276

Glycerol, DSMO

Front 277

Example of non-penetrating cryoprotective agents

Back 277

HES, PVP glucose

Front 278

RBC to be frozen should be less than _____ old

Back 278

6 days old

Front 279

Lifespan of glacierized RBC before and after thawing

Back 279

10 years , 24 hours

Front 280

Initial concentration Glycerol 40% w/v

Back 280

-80%

Front 281

Low concentration Glycerol 20% w/v

Back 281

-196%

Front 282

Deglycerolization is achieved by replacing glycerol with _____ amount of _____

Back 282

Decreasing, Saline

Front 283

Decrease in storage lesions

Back 283

2-3 DPG
pH
Glucose
ATP
Sodium

Hint 283

2PGAS

Front 284

Increase in storage lesions

Back 284

Hemoglobin
Ammonia
Lactate/Lactic acid
Potassium

Hint 284

HALP

Front 285

_____% is the minimum amount of RBC's must remain viable at the end of the permitted storage period

Back 285

70

Front 286

______ C is the transport storage temperature

Back 286

1-10

Front 287

Light spin _____ RPM or ____ g for _____minutes

Back 287

3200 RPM, 2000g, 3 minutes

Front 288

Hard spin _____ RPM or ____ g for _____minutes

Back 288

3500 RPM, 5000g, 5-8 minutes

Front 289

Blood product for Rapid blood loss

Back 289

Whole blood

Front 290

Blood product for anemia with large volume deficit

Back 290

Whole blood

Front 291

Blood product for neonatal exchange transfusion

Back 291

Whole Blood

Front 292

Whole blood unit should increase HCT by ____ and HGB for ______

Back 292

HCT: 3-5%
HGB: 1-15%

Front 293

Blood product for patients with symptomatic anemia and unable to tolerate sudden increase in blood volume

Back 293

Packed RBC's

Front 294

Blood product for neonates suffering from anemia due to FMH

Back 294

RBC aliquot

Front 295

Blood product for symptomatic anemia on patients with severe allergies/anaphylactic reaction

Back 295

Washed RBC's

Front 296

Blood product for patients who have anti-IgA antibody because of IgA deficiency

Back 296

Washed RBC's

Front 297

Blood product for prevention of non-hemolytic febrile transfusion reactions and exposure to CMV

Back 297

Frozen & deglycerolized RBC's

Front 298

Blood product for extension of time between transfusions in periodically transfused individuals (THALASSEMIA)

Back 298

Neocyte-enriched RBC's

Front 299

Blood product for prevention of HLA alloimmunization, TRALI and GVHP

Back 299

Leukoreduced RBC"s

Front 300

Blood product to replenish plasma proteins and coagulation factors

Back 300

Fresh frozen plasma

Front 301

Blood product for minimization of exposure to donor WBC, prevent febrile transfusion reaction caused by antibodies againts WBC's

Back 301

Leukoreduced rbc

Front 302

Fresh frozen plasma is procced for 6-8 hours, if 24 hours its called

Back 302

PF 24

Front 303

Blood product for inactive replicative machinery of the donor leukocyte

Back 303

Irradiated RBC

Front 304

Blood product for prevention of GVHD

Back 304

Irradiated RBC

Front 305

Most prone blood product to contamination

Back 305

Random donor platelet

Front 306

Minimum number of PLT in 1 unit in single donor platelet

Back 306

3x10^11 PLT

Front 307

Single donor platelet can increase PLT by

Back 307

30,000 to 60,000 PLT

Front 308

random donor platelet can increase PLT by

Back 308

5000-10000 PLT

Front 309

Blood product for von Willebrand disease, Hypofibrinogenemia, Hemophilia basically coagulation diseases

Back 309

Cryoprecipitate

Front 310

Blood product given to severely neutropenic patients

Back 310

Granulocyte concentrate

Front 311

Blood product for patients who have overwhelming sepsis

Back 311

Granulocyte concentrate

Front 312

Blood product for hypovolemic shocks and severe burns

Back 312

Single donor plasma

Front 313

Blood product for hemorrhagic shock and burn patients

Back 313

Volume expanders: Colloids and Crystaloids

Front 314

Storage temperature for Frozen RBC (Low)

Back 314

-120C-

Front 315

Storage temperature of Frozen RBC (High)

Back 315

-65C

Front 316

Storage temperature of FFP for 1 year

Back 316

-18C

Front 317

Storage temperature of FFP for 7 years

Back 317

-65C

Front 318

Transfusion procedure are checked and monitored by ____ nurses

Back 318

2

Front 319

_______ represent main cause of transfusion related deaths and acute HTR

Back 319

clerical error

Front 320

Specimen for transfusion must be collected within _____ of scheduled transfusion

Back 320

3 days

Front 321

Only ______ or _______ should be used as IV solutions to dilute blood components

Back 321

Isotonic saline or 5% albumin

Front 322

Use of _______ is PROHIBITED, since it it hypotonic and will cause RBC lysis

Back 322

5% dextrose in water

Front 323

Use of _______ is PROHIBITED, since it contains calcium and will initiate coagulation

Back 323

ringer solution

Front 324

First generation filter pore size of _______ um to remove gross clots and cell debris

Back 324

170-260

Front 325

Second generation filter pore size of ______ to remove microaggregates such as degenerated WBC's and call fragments

Back 325

20-40

Front 326

Third generation filters remove WBC leaving less than 5x10^6

Back 326

5x10^6

Front 327

Blood must NOT be warmed over

Back 327

38C

Front 328

ANY transfusion should be done within ____

Back 328

4 hours

Front 329

Blood can be reissued after returning from the ward IF

Back 329

the blood must not have been away from the blood bank for more than 30 minutes

Front 330

Filter to remove grass clots and cell debris

Back 330

first generation filters

Front 331

Filter to remove microaggregates such as degenerated WBC

Back 331

second generation

Front 332

Massive transfusion for adults units is within

Back 332

8-10 units, <24 hours

Front 333

Massive transfusion for infants units is within

Back 333

more than a half a unit

Front 334

Neonatal RBC transfusion blood needed is less than_____ days to reduce hyperkalemia and maximize 2-3 DPG

Back 334

7 days

Front 335

Done on a newborn infant suffering from anemia and hyperbilirubinemia due to HDN

Back 335

Exchange transfusion

Front 336

Performed when baby inside the mothers womb is suffering from severe anemia due to HDN

Back 336

Intrauterine transfusion

Front 337

Increase fluid in developing fetus

Back 337

Hydrops fetalis

Front 338

Intrauterine transfusion method by injecting the rbc into the fetal peroneal cavity

Back 338

Intraperitoneally

Front 339

Intrauterine method where RBC is directly injected into fetal umbilical vein

Front 340

Purpose of intrauterine transfusion

Back 340

Maintain fetal hemoglobin above 10 g/dl