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15 Cards in this Set
- Front
- Back
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What affect does a catalyst have on a reaction? Does it change to do this?
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Increases rate
Doesn't change |
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What is a cofactor/coenzyme? Subcategory (based on binding)?
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Add'l molecule required for enzymatic activity, usually a metal ion (REVERSIBLE BINDING)
If covalently bound, = prosthetic group |
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How do enzymes accelerate reactions (in terms of pathways a reaction can take)?
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Promote metabolism through desired pathways (ex: glucose--> glycolysis or to glycogen synthesis)
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What is the K value of a reaction? If K=2, what does that mean?
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Rate constant:
If K=2 then twice that starting amount of substrate is altered per second |
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ἐαυτοῦ, -ης
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himself; herself; itself
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What does the graph of a reversible first-order reaction look like?
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A<-->B
A and B concentrations equilibrate as reaction continues |
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What is the V of the following second order reactions:
2A-->C A+B-->C |
V=k[A]^2
V=k[A][B] |
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What is the accepted model of enzyme catalysis?
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Induced fit (NOT LOCK AND KEY); Enzyme and Substrate change conformation
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What are the assumption of M-M?
Hint: Concentrations, transition state, velocity measured |
[S]>[E]
[TS] does not change, rate at which it forms = rate at which it's degraded V measured = initial velocity |
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What is M-M eqn?
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V0=(Vmax*[S])/([S]+Km)
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What is Km in the M-M Equation?
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Michaelis constant:
Km=[S] at which v is 1/2 of vMax |
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On a double reciprocal plot (Lineweaver-Burk) where would you find the negative inverse of Km? The inverse of Vmax?
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Inverse of Vmax = y axis intercept
neg inverse of Km = x axis intercept |
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What occurs during competitive inhibition? Affect on Km?
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Inhibitor blocks substrate binding site, Km is INCREASED
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What occurs during non-competitive inhibition? Affect on Km?
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Inhibitor binds on non-substrate binding site, changes conformn of enzyme so it doesn't work as well. It's independent of [S] so Km is same but line is less steep on lineweaver burk plot
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What can you determine about the nature of an inhibitor from a graph?
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Whether it's competitive or not;
if it's competitive, Km will change as well as Vmax If it's non-competitive, Km will remain unchanged, Vmax will decrease Can't tell if it's reversible from graph |
