Biotech Test 1 Mcshan Review Ncs

Front 1

Southern and Northern Blots
a)uses...
b)can first be...
c)requires...
d)c) can either be...
e)alternative to b)

Back 1

a)nucleic acid hybridization to detect presence of a specific sequence
b)DNA/RNA first separated by size and charge then blotted onto memebrane
c)"labeled" probe to detect sequence
d)labeled radioactively or chemically
e)DNA/RNA blotted directly onto memebrane for probing

Front 2

Microarrays
a)def
b)probes can either be...

Back 2

a)hybridization analysis of mRNA or DNA on genome wide scale
b)PCR or lithography

Front 3

For ALL hybridization techniques what can be used to achieve clean results (3)

Back 3

1)temperature
2)salt []
c)detergents

Front 4

Computers can be used to assist in analysis of DNA via...(2)

Back 4

1)finding genes
2)assigning fxn (BLAST)

Front 5

Proteomics tells you

Back 5

WHEN AND IF genes are being transcribed and translated

Front 6

Key technologies in Proteomics (3)

Back 6

1)microarrays
2)mass spec
3)2D electrophoresis

Front 7

Phylogenetic analysis has to do w/....

Back 7

minimal essential genome

Front 8

Bioinformatics can ID...

Back 8

unique targets for chem, Abx, vaccines

Front 9

Bioinformatics def.

Back 9

comparison of DNA and proteins to databases online to ID and assign fxn to new genes

Front 10

ESTs
a)are detected by...(2)
b)derived from...

Back 10

1)microarrays
2)northern blots

3)isolating cDNAs from cells

Front 11

Therapeutically altering Gene expression (3 ways)

Back 11

1)antisense RNA
2)modified antisense oligonucleotides
3)siRNA

Front 12

Antisense RNA (2)

Back 12

1)single stranded DNA oligonucleotides
2)induces RNAase H

Front 13

Modified Antisense Oligonucleotides (2)

Back 13

1)chemically modified oligonucleotides
2)steric hindrace of translation

Front 14

siRNA (3)

Back 14

1)uses 2x stranded RNA
2)DICER enzyme
3)RISC complex