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14 Cards in this Set

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Southern and Northern Blots
a)uses...
b)can first be...
c)requires...
d)c) can either be...
e)alternative to b)
a)nucleic acid hybridization to detect presence of a specific sequence
b)DNA/RNA first separated by size and charge then blotted onto memebrane
c)"labeled" probe to detect sequence
d)labeled radioactively or chemically
e)DNA/RNA blotted directly onto memebrane for probing
Microarrays
a)def
b)probes can either be...
a)hybridization analysis of mRNA or DNA on genome wide scale
b)PCR or lithography
For ALL hybridization techniques what can be used to achieve clean results (3)
1)temperature
2)salt []
c)detergents
Computers can be used to assist in analysis of DNA via...(2)
1)finding genes
2)assigning fxn (BLAST)
Proteomics tells you
WHEN AND IF genes are being transcribed and translated
Key technologies in Proteomics (3)
1)microarrays
2)mass spec
3)2D electrophoresis
Phylogenetic analysis has to do w/....
minimal essential genome
Bioinformatics can ID...
unique targets for chem, Abx, vaccines
Bioinformatics def.
comparison of DNA and proteins to databases online to ID and assign fxn to new genes
ESTs
a)are detected by...(2)
b)derived from...
1)microarrays
2)northern blots

3)isolating cDNAs from cells
Therapeutically altering Gene expression (3 ways)
1)antisense RNA
2)modified antisense oligonucleotides
3)siRNA
Antisense RNA (2)
1)single stranded DNA oligonucleotides
2)induces RNAase H
Modified Antisense Oligonucleotides (2)
1)chemically modified oligonucleotides
2)steric hindrace of translation
siRNA (3)
1)uses 2x stranded RNA
2)DICER enzyme
3)RISC complex