Biom1009 - Dna Damage And Repair

Front 1

Name 2 mechanisms of Endogenous DNA damage.

Back 1

Replication Errors, Oxidative Deamination

Front 2

Exogenous DNA damage includes Viral and Physical mechanisms (such as hydrolysis or heat) and what other 2 factors?

Back 2

Radiation, Chemical

Front 3

Thymine dimers occur due to UVB light exposure. True or False?

Back 3

True

Front 4

Base oxidation is a mechanism of DNA damage. True or False?

Back 4

True

Front 5

DNA damage characterised by the cleavage of guanine from pentose is called what?

Back 5

Depurination

Front 6

DNA damage characterised by the removal of NH2 is called what?

Back 6

Deamination

Front 7

Single/double strand breaks are mechanisms of DNA damage. True or False?

Back 7

True

Front 8

Thymine dimers are NOT major causes of skin cancer. True or False?

Back 8

False

Front 9

Name two repair mechanisms used to correct Thymine dimers.

Back 9

Nucleotide Excision Repair, Photoreactivation

Front 10

photoreactivation uses the enzyme ____________ and requires __________ light.

Back 10

Photolyase, Blue

Front 11

Xeroderma Pigmentosum is a genetic disorder which leaves the individual with a predisposition to skin cancer. True or False?

Back 11

True

Front 12

Excision repair is the removal of the thymine dimer and replacing it with the standard T-T using DNA Polymerase III and DNA Ligase. True or False?

Back 12

False

Front 13

Excision repair is the removal of the thymine dimer and replacing it with the standard T-T using DNA Polymerase I and DNA Ligase. True or False?

Back 13

True

Front 14

Free radical damage causes base oxidation. True or False?

Back 14

True

Front 15

Reactive Oxygen Species (ROS) have one or more unpaired electrons, which makes them highly reactive. True or False?

Back 15

True

Front 16

Oxygen and Hydroxyl ions are ROS, yet Super Oxide, Peroxide and the Hydroxyl Radical are NOT ROS. True or False?

Back 16

False

Front 17

Oxygen and Hydroxyl ions are NOT ROS, yet Super Oxide, Peroxide and the Hydroxyl Radical are ROS. True or False?

Back 17

True

Front 18

Errors in replication are usually fixed by an array of proteins which remove mismatches. True or False?

Back 18

True

Front 19

Deamination of Guanine results in ___________ being formed.

Back 19

Xanthine

Front 20

Deamination of Adenine results in ___________ being formed.

Back 20

Hypoxanthine

Front 21

Bifunctional alkylating agents (such as Cisplatin and Carboplatin) can cause intra-strand linking and cross linking in DNA bases. True or False?

Back 21

True

Front 22

Radiation can ionise DNA. True or False?

Back 22

True

Front 23

Double strand breaks are solved via DNA Ligase. True or False?

Back 23

False

Front 24

Double strand breaks are solved via Homologous recombination. True or False?

Back 24

True

Front 25

Single strand breaks are fixed via Homologous recombination. True or False?

Back 25

False

Front 26

Single strand breaks are fixed by DNA Ligase. True or False?

Back 26

True