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50 Cards in this Set

  • Front
  • Back

Solvent

Does the dissolving

Solute

What is being dissolved

Deviation Formula

Experimental value minus theoretical value

Hydrophilic

Can dissolve in water because it is polar

Hydrophilic

Can be dissolved in water because it is polar

Hydrophobic

Cannot dissolve in water because it is non-polar

Meters

Length

Liters

Volume

Grams

Mass

Decimeter

1/10

Centimeter

1/100

Centimeter

1/100

Millimeter

1/1000

More then 7 carbons equals?

Hydrophobic

Acid

Releases hydrogen ions (H+) when dissolved in water (H2O)

Base

Releases hydroxide ions (OH-) when dissolved in water (H2O)

Buffers

Absorb the acid or base, thereby preventing a change in pH

Concentration =?

Solute/Solvent (mass/volume)


(Grams/Liters

More then 7 carbons =?

Hydrophobic

Less then 7 carbons equals =?

Hydrophilic

What color does a positive Benedicts test yield (from least to greatest)?

Green, Orange, Red

What colors do a positive Lugols test yield?

Blue and black

What color does a positive Biurets test yield?

Purple

What color does a positive Sudan III test yield?

Orange and Red

What color does a positive Sudan III test yield?

Orange and Red

What do you test for in a benedicts test?

Monosaccharide Carbohydrates (Glucose)

What color does a positive Sudan III test yield?

Orange and Red

What do you test for in a benedicts test?

Monosaccharide Carbohydrates (Glucose)

What do you test for in a Lugols test?

Polysaccharide Carbohydrates (Starch)

What do you test for in a Biurets test?

Proteins

What do you test for in a Sudan III test?

Lipids

Is the lipids test hydrophilic or hydrophobic?

Hydrophobic

How does the Biurets test work?

Peptide bond

How does the Biurets test work?

Peptide bond

What is a controlled group?

A group of subjects that do not receive the treatment

What is an experimental group?

A group of subjects that do receive the treatment

What is a hypothesis?

A prediction or observation that can be tested

What is a hypothesis?

A prediction or observation that can be tested

What are the 5 steps of the scientific method?

1. Make observations


2. Ask questions


3. Formulate a hypothesis


4. Test hypothesis


5. Results and conclusions

Independent Variables

Are known before the experiment

Independent Variables

Are known before the experiment

Dependent Variables

Depend on the independent variable for their outcomes

What color does a negative Sudan III test yield?

Red

What color does a negative Biurets test yield?

Blue

What color does a negative Biurets test yield?

Blue

What color does a negative Benedicts test yield?

Blue

What color does a negative Biurets test yield?

Blue

What color does a negative Benedicts test yield?

Blue

What color does a negative Lugols test yield?

Orange and Brown

Steps to preforming a DNA extraction?

1. Mash subject to be extracted to break cell wall



2. Add extraction buffer (ethanol) to dissolve cell and nuclear membranes