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11 Cards in this Set
- Front
- Back
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clone
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make copies
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you can clone a gene by...
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inserting it into a plasmid and putting it into a bacteria host cell by using pcr to make copies
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you clone individuals by...
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putting the nucleus of a cell into a stem/egg cell from another individual
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restriction enzyme
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an enzyme that cuts DNA at particular nucleotide sequences
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Restriction enzymes sometimes leave....
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Sticky ends
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Plasmid
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a small circle of DNA containing a few genes
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Gene of interest
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the gene you want to clone and study to produce a drug as insulin
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recombinant DNA
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a plasmid that contains a gene of interest from and organism different from the host organism
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vector
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DNa that can carry your gene of interest into its host cell
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screen
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a technique that lets you tell whether you got the gene of interest into the host cell
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PCR (polymerase chain reaction)
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clones a gene by using primers and DNA replication
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