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34 Cards in this Set
- Front
- Back
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Psychrolphiles
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cold loving 15C
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Mesophiles
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moderate 25-40C
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Thermophiles
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hot springs 50-60C
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Hyperthermophiles
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Extreme heat 90C+
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Psychrotrophs
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food spoilage 20-30C
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Obligate anerobe
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requires O2
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microaerophile
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requires low O2
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Facultative anaerobe
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grows with or without O2 and will use if present
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Obligae anaerobe
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grows only in abscense of O2
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Aerotolerant Anaerobe
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grows in presence of O2 but does not use it
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Lag phase
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period of no growth enzyme and DNA synthesis occurs
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Log phase
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exponential growth phase
cells grow at maximum rate most sensitive to penicillin and radiation |
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stationary phase
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# dying = # dividing
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Death phase
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number dying exceeds number dividing
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SPC (standard plate count)
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Counts # of colonies on agar
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Filtration
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Counts colonies on nutrient agar after capturing bacteria on a filter
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MPN (most probable number)
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statistically determines 3 of coli forms
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Direct count
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determine the number of bacteria per ML of sample
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Turbidity
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indirect method # of microbes is directly proportional to amt of light scattered
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metabolic activity
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measures amount of metabloite consumed
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Dry weight
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measures dry weight of filament microbes
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Sterilization
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killing of all forms of life
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disinfections
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destruction of vegetative forms, disinfectant and antiseptic
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sanitization
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reducing number of microbes to acceptabel health levels
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degerming
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mechanical removal of micobes
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Moist Heat
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denatures protein
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dry heat
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denatures protein/oxidizes
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cold
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refridgeration, lyophilization, deep freezing
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filtration
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uses filters
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osmotic pressure
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pressure from salts and suagrs force water out of microbes
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plasmolysis
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food preservation
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high pressire
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changes molecular structire of carbs and proteins
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desiccation
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drying, distrupts metabolism
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radiation
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ionizing, non ionizing, and UV
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