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28 Cards in this Set
- Front
- Back
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Dry Mount |
Specimens are placed on slide and cover slip over it |
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Wet Mount |
Specimens suspended in liquid or oil and cover slip over |
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Squash Slides |
Wet mount prepared and cover slip is pressed down on |
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Smear Slides |
Edge of slide used to smear sample over slide |
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Objective Lens |
Lens near specimen |
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Eyepiece Lens |
Lens used to view specimen |
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Gram Stain Technique Target |
Stains gram positive bacteria (thick cell walls) as blue or violet |
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Gram Stain Technique Method |
Crystal Violet to stain, Iodine to fix and washed with alcohol |
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Gram Stain Technique Counterstain |
Safranin dye for gram negative bacteria, appearing red |
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Counterstain |
Second stain used for contrasting colour e.g. safranin dye in gram staining |
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Fixing Slides |
Chemicals used to preserve specimens e.g. formaldehyde |
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Sectioning Slides |
Specimens dehydrated with alcohol and placed in mould to form a block, then sliced |
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Staining |
Treated with stains to show structures |
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Mounting |
Specimens secured to a slide |
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Magnification |
How many times larger the image is than the actual size |
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Resolution |
The ability to see individual objects as separate entities |
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Magnification calculation |
Magnification=size of image/actual size |
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Eyepiece graticule |
Used to measure size of sample under microscope, used with stage micrometer |
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1000 Micrometres |
Um, 1mm |
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1000 nanometres |
nm, 1 micrometer (um) |
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Compound light microscope |
Regular microscope, used with light |
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Artefacts |
Objects created through specimen processing |
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Transmission Electron Microscope |
Beam of electrons passed through specimen, focused to produce image (r power 0.5 nm) |
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Scanning Electron Microscope |
Beam of electrons reflected from specimen (r power 3-10 nm) |
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Light vs Electron Comparison (8) |
Expense Portability Preparation Vacuum Colour Magnification Resolving power Specimens |
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Light vs Electron Magnification |
x2000 vs x500 000 |
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Light vs Electron Resolving Power |
200nm vs 0.5/3-10 nm |
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Laser scanning confocal microscopy |
Spot of focused light moved across specimen, illuminating dye |
