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95 Cards in this Set

  • Front
  • Back
Prokaryotic Cells
- no membrane bound oragnelles
- a lot smaller compared to eukaryotes
Bacteria
- prokaryote
- constitute a large domain of prokaryotic microorganisms
- contains peptidoglycan in cell wall
Archaea
- prokaryote
- single celled microorganism
- does not have a usual cell wall
- does not contain peptidoglycan in cell wall
- some species can live in extreme environments
Prokaryote that inhabits (lives) in the Great Salt Lake in Utah.
Halobacterium (genus)
The most common shapes or prokaryotes
- Spherical (cocci)
- Rod-shaped ( Bacilli)
- spiral ( spirilla)
cell surface structures
- cell wall of prokaryotes differ from eukaryotes in terms of structure
Eukaryote Cell wall structure (plants and fungi)
- made from cellulose or CHITIN
Most Bacterial cell walls contain
- Peptidoglycan
Peptidoglycan
- polymer composed of modified sugar crossed-linked by short polypeptides
- encloses the entire bacterium and anchors other molecules that extend from its surface
Archaeal cell wall
- does not have peptidoglycan
- contains a variety of polysacchrides and proteins
Gram staining
- quickly determining if cell is gram negative or positive
- developed by a Danish physician Hans Christian Gram
- If stain stays, bacteria is Gram positive. If the stain doesn't stay, it is gram negative.
Gram positive
- the stain stays
- have simpler cell walls
- contain a large amount of peptidoglycan
Gram negative
- has less peptidoglycan
- structurally more complex
- outer layer contains lipopolysacchrides
- lipid portion of lipopolysacchride are toxic, causing fever or shock
-impedes entry of drugs due to the outer due to the outer membrane (more resistant to drugs
lipopolusacchrides
carbohydrates bonded to lipids
penicillin
- attacks cell wall of bacteria
- inhibits peptidoglycan cross linking
- resulting cell wall may not be functional specially in gram positive bacterias
Gram positive and negative (picture)
cell wall of prokaryotes
- many are surrounded by a layer of polysacchrides or protien
- capsule
- slime layer
capsule
- dense, well defined layer of polysacchride or protien layer present in most prokaryotes
slime layer
- less well organized version of the capsule
Both capsule and slime layer
- enables prokaryotes to adhere to their substrate or to other individuals in a colony
- some protect against dehydration
- some shield pathogenic prokaryotes from attacks by their host's immune system
pili (sex pili)
- longer than fimbriae
- allow prokaryote to exchange DNA
Fimbriae (attachment pili)
- hairlike appendages used by some prokaryotes to stick to their substrate or to one another
- shorter than pili
taxis
- almost half of all prokaryotes are capable off
- a directed movement toward or away from a stimulus
- cells may use flagella as a motility structure
prokaryotes
- DNA is circular
- no membrane bound oraganelle
- much smaller compared to eukaryotes
- evolve/ reproduce quickly (short generation time) by binary fission (1-3 hours)
- asexual
- chormosome is located in the nucleoid
eukaryotic cell
- have memrbrane bound organelles
- linear DNA
plasmids
- much smaller rings of independently replicating DNA molecules, carrying only a few genes
binary fission
- a single cell divides into 2
- exponential
- prokaryotes can divide within 1-3 hours
- some can produce a new generation within 20 minutes
endospore
- is a dormant, tough, and non-reproductive structure produced by certain bacteria
- triggered by a lack of nutrients, and usually occurs in Gram-positive bacteria
- Endospores enable bacteria to lie dormant for extended periods, even centuries
Obligate Aerobe
- cannot live withour oxygen
- require oxygen for cellular respiration
Obligate anaerobe
- poisoned by oxygen
- use fermintation or anaerobic respiration
Facultive anaerobes
- can survive with or without oxygen
- grows better with oxygen
Nitrogen
- essential for the production of amino acids and nucleic acids in all organisms
Nitrogen Fixation
- conversion of nitrogen to ammonia
Nitrogen fixation
- prokaryotes can metabolize Nitrogen in a wide variety of forms
- some archaea (cyanobacteria/ methanogens), convert atmospheric nitrogen to ammonia
nitrogen-fixing prokaryotes
- can increase the nitrogen available for plants which cannot use atmospheric nitrogen but can use the nitrogen compounds from ammonia
Metabolic Cooperation
cooperation between prokaryotic cells that allow them to use environmental resources they could not use as individual cells.
heterocysts
- specialized cells that carry out only nitrogen fixation
Biofilms
- surface coating colonies where metabolic cooperation between different prokaryotic species occur.
- cells in a biofilm secrete signaling molecules that recriut nearby cells, causing the colonies to grow
Archaea
share certain traits with bacteria and other traits with eukaryotes
extemophiles
- live in extreme environments
- "lovers" of extreme conditions
- extreme halophiles
- extreme thermophiles
extreme halophiles
- live in highly saline environments (salty) (Great Salt Lake and the Dead Sea)
- ex. is a Halobacterium
extreme thermophiles
- Thrive in very hot environments
- ex. is Sulfolobus, lives in sulfur rich volcanic springs as hot as 90C. temperatures this high prevents the DNA of most organism from being a double helix.
methanogens
- produce methane
- anaerobes
- arhaea that live in moderate environments
chemical reycling
.
decomposers
- chemoheterotrophic prokaryote function as a decomposer
- breaking down of dead organisms as well as waste products and thereby unlocking supplies of carbon, nitrogen, and other elements.
Ecological Interactions
-prokaryotes play a central role in many ecological interactions
Symbiosis
an ecological relationship in which two species live in close contact with each other
host
larger organism in a symbiotic relationship
symbiont
smaller organism in a symbiotic relationship
mutualism
An ecological interaction between two species in which both benefit
commensalism
an ecological relationship in which one species benefits while the other is not harmed or helped in any significant way
parasitism
- an ecological relationship in which a parasite eats the cell contents, tissues, or body fluids of its host.
- as a group, parasites harm but does not usually kill their host
pathogens
parasites that cause disease, many of which are prokaryotes
Exotoxins
- proteins secreted by certain bacteria and other organisms
Endotoxins
- lypopolusacchride components of the outer membrane of gram negative bacteria.
- endotoxins are only released when the bacteria die and their cell walls breakdown.
metabollic diversity of prokartyoes
-Autotroph ( photoautotroph and chemoautotroph)
- Heterotroph (photoheterotroph and chemoheterotroph )
autotroph
- Photoautotroph
- Chemoautotroph
Photoautotroph
- Energy source is Light\
- Carbon source is Carbon Dioxide\
- Photosynthetic prokaryote (cyanobacteria)( eg. protist (algae))
Chemoautotroph
- Energy source are Inorganic chemicals
- Carbon source is Carbon Dioxide
- eg. Sulfolobus
Heterotroph
- Photoheterotroph
- Chemoheterotroph
Photoheterotroph
- Energy source is Light
- Carbon source are Organic compounds
- eg. of organism- certain prokaryotes such as Rhodobacter
Chemoheterotroph
- Energy source are organic compounds
- carbon source are organic compounds
- eg. of organism - many prokaryote and protists ( fungi, animals and some plants
Molecular systematics
:D
Universal ancestor
Polymerase chain reaction (PCR)
- allowed for more rapid sequencing of prokaryote genomes
- a handful of soil may contain 10,000 prokaryotic species
- Horizontal gene transfer (HGT) between prokaryotes obscures the root of the tree of life
Prokaryotes in Research and Technology
HAHAHA
Experiments using prokaryotes
Have led to important advances in DNA technology
Bioremediation
- the use of organisms to remove pollutants from the environment
Some uses of prokaryotes
- Recovery of metals from ores
- Synthesis of vitamins
- Production of antibiotics, hormones, and other products
Hypothesis of the origin of eukaryotes
MUAHGAHAHAH
oldest fossil of Eukaryote
- oldest fossil date back to 2.1 billion years
Hypothesis of endosymbiosis
- proposes that mitochondria and plastids ( chloroplasts) were formerly small prokaryotes living within larger host cells
endosysmbiont
a cell that lives within a host cell
Serial endosymbiosis
- supposes that mitochondria evolved before plastids through a sequence of endosymbiotic events
Endosymbiosis in Eukaryotic Evolution
Endosymbiosis in Eukaryotic Evolution ( The way Guppy likes it ;) )
1. Large prokaryote's plasma membrane infolds. Large prokaryote turns into a "primitive heterotrophic eukaryotic cell".
2. Primitive heterotrophic eukaryotic cell, swallows a "heterotrophic aerobic free living prokayote". For some reason, the Large eukaryote doesn't digest it, forming a symbiotic relationship over time. Turns into mitochondria. The large eukartyotic cell turns into a " Large heterotrophic Eurkaryotic cell".
3. "LHEC", engulfs an autotroph (photosynthetic prokaryote). Does not digest it, and over time forms a symbiotic relationship. The prokaryote turns into a plastid (eg. chloroplast)
4. Later evolved to be either a Heterotrophic protist or a autotrophic protist.
Mitochondria
evolved by endosymbiosis of an aerobic prokaryote
Plastid
evolved by endosymbiosis of a photosynthetic cyanobacterium
Key evidence supporting an endosymbiotic origin of mitochondria and plastids:
- Similarities in inner membrane structures and functions
- Division is similar in these organelles and some prokaryotes
- These organelles transcribe and translate their own DNA
- Their ribosomes are more similar to prokaryotic than eukaryotic ribosomes
Protist
- the informal name of the kingdom of mostly unicellular eukaryotes
- eukaryotes
- some are colonial and multicellular species
- more structural and functional diversity than any other eukaryotes
- can be very complex
- can reproduce sexually or asexually
Nutritional forms of Protista
- photoautotroph
- heterotroph
- mixotroph
photoautotroph
- contain chloroplast
heterotroph
- absorb organic molecules or ingest larger food particles
mixotroph
- combine photosynthesis and heterotrophic nutrition
Five Supergroups of Eukaryotes
It is believed that animal and fungal cells
arose from ancestral protists that did not engulf cyanobacteria (photosynthetic prokaryote) after mitochondria, therefore, remained heterotrophic
Plants may have arisen
from photosynthetic protists containing plastids
Protista
- live in a aquatic or damp environments (ponds, lakes, and oceans)
Amoeba and paranecium
- unicellular heterotrophic protists
- amoeba by extension of their cytoplasm in pseudopods and engufs food particles
- paramecia move by beating of cillia
Photosynthetic protists such as EUGLENA
- found in fresh water enviroments and move by use of flagellum.
- contains chloroplast, therefore, it can make its own food
slime molds and water molds
- colonial heterotrophic protists
- involved in decomposition of organic materials
- slime molds are clumps of amoeba like cells
- often found on rotting logs
Algae
- mainly multicellular protists
- photosynthetic
- 3 main groups: Chlorophyta, Phaeophyta, Rhodophyta
Chlorophyta
- green algae
- unicellular
-most abundant and contain chlorophyl a and b.
- store food as starch in plastid
- have a cellulose wall, closely related to plants
Phaeophyta
- brown algae
- almost exclusively marine
- have chlorophyl a and c
Rhodophyta
- red algae
- contain chlorophyll a and some have chlorophyll d
- accessory pigment that give them their reddish colour
- found in deeper dephts than the chlorophyta and phaetophyta