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95 Cards in this Set
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- Back
Prokaryotic Cells
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- no membrane bound oragnelles
- a lot smaller compared to eukaryotes |
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Bacteria
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- prokaryote
- constitute a large domain of prokaryotic microorganisms - contains peptidoglycan in cell wall |
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Archaea
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- prokaryote
- single celled microorganism - does not have a usual cell wall - does not contain peptidoglycan in cell wall - some species can live in extreme environments |
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Prokaryote that inhabits (lives) in the Great Salt Lake in Utah.
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Halobacterium (genus)
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The most common shapes or prokaryotes
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- Spherical (cocci)
- Rod-shaped ( Bacilli) - spiral ( spirilla) |
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cell surface structures
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- cell wall of prokaryotes differ from eukaryotes in terms of structure
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Eukaryote Cell wall structure (plants and fungi)
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- made from cellulose or CHITIN
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Most Bacterial cell walls contain
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- Peptidoglycan
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Peptidoglycan
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- polymer composed of modified sugar crossed-linked by short polypeptides
- encloses the entire bacterium and anchors other molecules that extend from its surface |
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Archaeal cell wall
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- does not have peptidoglycan
- contains a variety of polysacchrides and proteins |
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Gram staining
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- quickly determining if cell is gram negative or positive
- developed by a Danish physician Hans Christian Gram - If stain stays, bacteria is Gram positive. If the stain doesn't stay, it is gram negative. |
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Gram positive
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- the stain stays
- have simpler cell walls - contain a large amount of peptidoglycan |
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Gram negative
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- has less peptidoglycan
- structurally more complex - outer layer contains lipopolysacchrides - lipid portion of lipopolysacchride are toxic, causing fever or shock -impedes entry of drugs due to the outer due to the outer membrane (more resistant to drugs |
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lipopolusacchrides
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carbohydrates bonded to lipids
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penicillin
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- attacks cell wall of bacteria
- inhibits peptidoglycan cross linking - resulting cell wall may not be functional specially in gram positive bacterias |
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Gram positive and negative (picture)
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cell wall of prokaryotes
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- many are surrounded by a layer of polysacchrides or protien
- capsule - slime layer |
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capsule
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- dense, well defined layer of polysacchride or protien layer present in most prokaryotes
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slime layer
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- less well organized version of the capsule
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Both capsule and slime layer
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- enables prokaryotes to adhere to their substrate or to other individuals in a colony
- some protect against dehydration - some shield pathogenic prokaryotes from attacks by their host's immune system |
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pili (sex pili)
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- longer than fimbriae
- allow prokaryote to exchange DNA |
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Fimbriae (attachment pili)
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- hairlike appendages used by some prokaryotes to stick to their substrate or to one another
- shorter than pili |
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taxis
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- almost half of all prokaryotes are capable off
- a directed movement toward or away from a stimulus - cells may use flagella as a motility structure |
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prokaryotes
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- DNA is circular
- no membrane bound oraganelle - much smaller compared to eukaryotes - evolve/ reproduce quickly (short generation time) by binary fission (1-3 hours) - asexual - chormosome is located in the nucleoid |
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eukaryotic cell
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- have memrbrane bound organelles
- linear DNA |
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plasmids
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- much smaller rings of independently replicating DNA molecules, carrying only a few genes
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binary fission
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- a single cell divides into 2
- exponential - prokaryotes can divide within 1-3 hours - some can produce a new generation within 20 minutes |
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endospore
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- is a dormant, tough, and non-reproductive structure produced by certain bacteria
- triggered by a lack of nutrients, and usually occurs in Gram-positive bacteria - Endospores enable bacteria to lie dormant for extended periods, even centuries |
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Obligate Aerobe
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- cannot live withour oxygen
- require oxygen for cellular respiration |
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Obligate anaerobe
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- poisoned by oxygen
- use fermintation or anaerobic respiration |
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Facultive anaerobes
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- can survive with or without oxygen
- grows better with oxygen |
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Nitrogen
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- essential for the production of amino acids and nucleic acids in all organisms
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Nitrogen Fixation
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- conversion of nitrogen to ammonia
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Nitrogen fixation
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- prokaryotes can metabolize Nitrogen in a wide variety of forms
- some archaea (cyanobacteria/ methanogens), convert atmospheric nitrogen to ammonia |
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nitrogen-fixing prokaryotes
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- can increase the nitrogen available for plants which cannot use atmospheric nitrogen but can use the nitrogen compounds from ammonia
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Metabolic Cooperation
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cooperation between prokaryotic cells that allow them to use environmental resources they could not use as individual cells.
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heterocysts
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- specialized cells that carry out only nitrogen fixation
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Biofilms
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- surface coating colonies where metabolic cooperation between different prokaryotic species occur.
- cells in a biofilm secrete signaling molecules that recriut nearby cells, causing the colonies to grow |
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Archaea
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share certain traits with bacteria and other traits with eukaryotes
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extemophiles
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- live in extreme environments
- "lovers" of extreme conditions - extreme halophiles - extreme thermophiles |
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extreme halophiles
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- live in highly saline environments (salty) (Great Salt Lake and the Dead Sea)
- ex. is a Halobacterium |
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extreme thermophiles
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- Thrive in very hot environments
- ex. is Sulfolobus, lives in sulfur rich volcanic springs as hot as 90C. temperatures this high prevents the DNA of most organism from being a double helix. |
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methanogens
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- produce methane
- anaerobes - arhaea that live in moderate environments |
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chemical reycling
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.
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decomposers
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- chemoheterotrophic prokaryote function as a decomposer
- breaking down of dead organisms as well as waste products and thereby unlocking supplies of carbon, nitrogen, and other elements. |
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Ecological Interactions
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-prokaryotes play a central role in many ecological interactions
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Symbiosis
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an ecological relationship in which two species live in close contact with each other
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host
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larger organism in a symbiotic relationship
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symbiont
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smaller organism in a symbiotic relationship
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mutualism
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An ecological interaction between two species in which both benefit
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commensalism
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an ecological relationship in which one species benefits while the other is not harmed or helped in any significant way
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parasitism
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- an ecological relationship in which a parasite eats the cell contents, tissues, or body fluids of its host.
- as a group, parasites harm but does not usually kill their host |
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pathogens
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parasites that cause disease, many of which are prokaryotes
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Exotoxins
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- proteins secreted by certain bacteria and other organisms
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Endotoxins
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- lypopolusacchride components of the outer membrane of gram negative bacteria.
- endotoxins are only released when the bacteria die and their cell walls breakdown. |
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metabollic diversity of prokartyoes
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-Autotroph ( photoautotroph and chemoautotroph)
- Heterotroph (photoheterotroph and chemoheterotroph ) |
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autotroph
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- Photoautotroph
- Chemoautotroph |
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Photoautotroph
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- Energy source is Light\
- Carbon source is Carbon Dioxide\ - Photosynthetic prokaryote (cyanobacteria)( eg. protist (algae)) |
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Chemoautotroph
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- Energy source are Inorganic chemicals
- Carbon source is Carbon Dioxide - eg. Sulfolobus |
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Heterotroph
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- Photoheterotroph
- Chemoheterotroph |
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Photoheterotroph
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- Energy source is Light
- Carbon source are Organic compounds - eg. of organism- certain prokaryotes such as Rhodobacter |
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Chemoheterotroph
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- Energy source are organic compounds
- carbon source are organic compounds - eg. of organism - many prokaryote and protists ( fungi, animals and some plants |
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Molecular systematics
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:D
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Universal ancestor
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Polymerase chain reaction (PCR)
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- allowed for more rapid sequencing of prokaryote genomes
- a handful of soil may contain 10,000 prokaryotic species - Horizontal gene transfer (HGT) between prokaryotes obscures the root of the tree of life |
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Prokaryotes in Research and Technology
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HAHAHA
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Experiments using prokaryotes
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Have led to important advances in DNA technology
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Bioremediation
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- the use of organisms to remove pollutants from the environment
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Some uses of prokaryotes
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- Recovery of metals from ores
- Synthesis of vitamins - Production of antibiotics, hormones, and other products |
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Hypothesis of the origin of eukaryotes
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MUAHGAHAHAH
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oldest fossil of Eukaryote
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- oldest fossil date back to 2.1 billion years
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Hypothesis of endosymbiosis
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- proposes that mitochondria and plastids ( chloroplasts) were formerly small prokaryotes living within larger host cells
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endosysmbiont
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a cell that lives within a host cell
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Serial endosymbiosis
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- supposes that mitochondria evolved before plastids through a sequence of endosymbiotic events
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Endosymbiosis in Eukaryotic Evolution
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Endosymbiosis in Eukaryotic Evolution ( The way Guppy likes it ;) )
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1. Large prokaryote's plasma membrane infolds. Large prokaryote turns into a "primitive heterotrophic eukaryotic cell".
2. Primitive heterotrophic eukaryotic cell, swallows a "heterotrophic aerobic free living prokayote". For some reason, the Large eukaryote doesn't digest it, forming a symbiotic relationship over time. Turns into mitochondria. The large eukartyotic cell turns into a " Large heterotrophic Eurkaryotic cell". 3. "LHEC", engulfs an autotroph (photosynthetic prokaryote). Does not digest it, and over time forms a symbiotic relationship. The prokaryote turns into a plastid (eg. chloroplast) 4. Later evolved to be either a Heterotrophic protist or a autotrophic protist. |
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Mitochondria
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evolved by endosymbiosis of an aerobic prokaryote
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Plastid
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evolved by endosymbiosis of a photosynthetic cyanobacterium
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Key evidence supporting an endosymbiotic origin of mitochondria and plastids:
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- Similarities in inner membrane structures and functions
- Division is similar in these organelles and some prokaryotes - These organelles transcribe and translate their own DNA - Their ribosomes are more similar to prokaryotic than eukaryotic ribosomes |
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Protist
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- the informal name of the kingdom of mostly unicellular eukaryotes
- eukaryotes - some are colonial and multicellular species - more structural and functional diversity than any other eukaryotes - can be very complex - can reproduce sexually or asexually |
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Nutritional forms of Protista
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- photoautotroph
- heterotroph - mixotroph |
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photoautotroph
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- contain chloroplast
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heterotroph
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- absorb organic molecules or ingest larger food particles
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mixotroph
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- combine photosynthesis and heterotrophic nutrition
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Five Supergroups of Eukaryotes
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It is believed that animal and fungal cells
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arose from ancestral protists that did not engulf cyanobacteria (photosynthetic prokaryote) after mitochondria, therefore, remained heterotrophic
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Plants may have arisen
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from photosynthetic protists containing plastids
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Protista
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- live in a aquatic or damp environments (ponds, lakes, and oceans)
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Amoeba and paranecium
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- unicellular heterotrophic protists
- amoeba by extension of their cytoplasm in pseudopods and engufs food particles - paramecia move by beating of cillia |
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Photosynthetic protists such as EUGLENA
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- found in fresh water enviroments and move by use of flagellum.
- contains chloroplast, therefore, it can make its own food |
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slime molds and water molds
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- colonial heterotrophic protists
- involved in decomposition of organic materials - slime molds are clumps of amoeba like cells - often found on rotting logs |
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Algae
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- mainly multicellular protists
- photosynthetic - 3 main groups: Chlorophyta, Phaeophyta, Rhodophyta |
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Chlorophyta
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- green algae
- unicellular -most abundant and contain chlorophyl a and b. - store food as starch in plastid - have a cellulose wall, closely related to plants |
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Phaeophyta
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- brown algae
- almost exclusively marine - have chlorophyl a and c |
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Rhodophyta
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- red algae
- contain chlorophyll a and some have chlorophyll d - accessory pigment that give them their reddish colour - found in deeper dephts than the chlorophyta and phaetophyta |