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44 Cards in this Set

  • Front
  • Back
Define Organelle
Functionally and distinct part of a cell
Define Compartmentilisation
Organelles themselves are surrounded by membranes so that their activities can be separated from the surrounding cytoplasm
Define Division of Labor
Sharing of work between different specialized organelles
Describe the features of a Nucleus
Largest Organelle between 10 to 20 µm in diameter

Enclosed be an nuclear envelope of 2 membranes perforated by nuclear pores

Outer membrane continuous with ER

Contains Chromatin and Nucleolus
What is the function of a nucleus?
Chromosomes contain DNA

DNA is organised into genes which control all the activities of the cell

The nucleolus manufactures ribosomes
Describe the features of Rough ER
System of flattered, membrane-bound sacks called cisternae, forming tubes and sheets with ribosomes found on the surface

Continuous with the outer membrane of the nuclear envelope
What is the function of Rough ER
Transport proteins are made by the ribosomes through the cisternae
Describe the features of Smooth ER
System of flattered, membrane-bound sacks called cisternae, forming tubes and sheets

No ribosomes found on the surface
what is the function of a smooth ER?
Site of lipid and steroid synthesis
Describe the features of a Ribosome
Very small organelles (20nm)

Consist of a large and small subunit

Made of roughly equal parts of protein and rRNA

Slightly smaller ribosomes are found in mitochondria and chloroplasts
What is the function of a Ribosome?
Site of protein synthesis

Either bound to ER or lie free in the cytoplasm

May form polysomes (polyribosomes)
Describe the features of a Mitochondria
rod shaped, 1 to 10µm in diameter

Surrounded by an envelope of 2 membranes

Inner membrane folded to form Cristae

Contains a matrix with a few ribosomes, a circular DNA molecule and phosphate granules
What is the function of a Mitochondria?
In aerobic respiration, cristae is a site of oxidative phosphorylation and electron transport

Matrix is a part of Kreb's cycle

Production of ATP
Describe the features of a Golgi Apparatus
Stack of flattered membrane-bound sacs called cisternae, continuously formed at one end and budded off at the other end as vesicles
What is the function of a Golgi Apparatus?
Internal processing and transport system

Golgi vesicles transport the materials to the other part of the cell or to the cell surface membrane for secretion


Makes Lysosomes
Describe the features of a Lysosome
Up to 1µm in diameter

Simple spherical sac bounded by a single membrane and containing digestive (hydrolytic) enzymes

No internal structures visible
What is the function of a Lysosome?
Concerned with the breakdown of structures or molecules
e.g. get rid of old organelles and digest bacteria
Describe the features of a Cell (Plasma) Membrane
About 7nm in thickness

Trilaminar appearance
What is the function of a Cell (Plasma) Membrane
A partially permeable membrane controlling the import and export of selected chemicals between the cell and its environment
Describe the features of a Cell Wall
A rigid wall surrounding the cell, consisting of cellulose, microfibrils and ma be secondarily thickened in some cells
What is the function of a Cell Wall?
Provides mechanical support and protection

Allows a pressure to be developed which aids in support

Prevents Osmotic bursting of the cell

Pathway movement of water and mineral salts

Freely permeable
What is a Middle Lamina?
Thin layer of pectic substances
What is the function of a middle lamina?
cements neighboring cells together
What is a plasmodesma?
A fine cytoplasmic thread linking the cytoplasm of 2 neighboring cells through a fine pore in the cell walls, the pore is lined with the cell surface membrane
What is the function of a plasmodesma?
Enables a continuous system of cytoplasm, the symplast, to be formed between neighboring cells for transport of substances between cells
Describe the features of a Chloroplast
Biconvex disc, 2-10µm thick

Large plastid containing chlorophyll and carries out photosynthesis

Surrounded be an envelope ot 2 membranes and contains a gel-like stroma through which runs a system of membranes that are stacked in places to form grana. May store starch

Stroma also contains ribosomes, circular DNA molecule and lipid droplets
What is the function of a chloroplast?
The organelle in which photosynthesis takes pace, light energy is converted to chemical energy
Describe the features of a Large Central Vacuole
A sac bounded by a single membrane called the tonoplast

Contains cell sap, a concentrated solution of various substances, such as mineral salts, sugar, pigments, organic acid and enzymes

Typically large in mature cells
What is the function of a Large Central Vacuole
Storage of various substances including waste products

Makes an important contribution to the osmotic properties of the cell

Sometimes it functions as a Lysosome
What are the features of a Centriole
Small hallow cylinder about 0.4µm long and 0.2µm in diameter that occur in pairs inmost animal cells

Formed by a ring of microtubulus

"Triplets of nine" arrangement
What is the function of a Centriole?
These Microtubules are used to grow the spindle fibers for nuclear division
What are Basal bodies, cilia and flagella?
Long, thin extensions that can move in a wale like manner

Long and few: Flagella
Many and short: Cilia

Usually 3-4µm long, 0.2µm in diameter

Covered with an extension of the plasma membrane and it contains microtubules that extend throughout its length
What are the functions of a Basal bodies, cilia and flagella?
To move substances around the cell or to move the cell itself as the cilia beat
Define magnification
The number of times larger, when image is compared with the real size of the object
what is the formula for magnification?
Size of image/Actual size of specimen
Define Resolution
The ability to distinguish between 2 separate points
What is the Maximum resolution of a light microscope?
200nm
What is the Maximum resolution of a electron microscope?
0.5nm
What are the 2 reasons for using electrons in electron microscope?
Their wavelength is extremely short

They are Negatively charged, therefore they can be focused easily using electromagnets
what are the 2 types of electron microscopes?
Transmission electron microscope (TEM)

Scanning electron microscope (SEM)
What are the Difficulties in using an electron microscope?
The specimen ad the florescent screen must be seen in vacuum

All specimen must be dehydrated because water boils at room temperature in vacuum
What is the difference between a prokaryotic cell and a eukaryotic cell?
Eukaryotic cells are larger and the contain a nucleus

Prokaryotic cells are smaller and their DNA lies freely in the cytoplasm
What are the Features in Prokaryotic cells?
Size: Smaller cells, about 0.5 - 5µm

DNA:
Circular, lies free in cytoplasm
Naked DNA
May contain Plasmid

Ribosomes: smaller, 18nm

ER: None

Cell Organelles: very few organelles
2-membrane organelles absent

Cell wall: always present
What are the Features in Eukaryotic cells?
Size: bigger, about 40µm

DNA:
not circular and its inside the nucleus
associated with protein

Ribosomes: larger, 22nm

ER: present

Cell Organelles:
Many cell organelles
No membrane(ribosomes)
Single membrane(lysosome, Ga, Vacuoles)
2-membraned(nucleus, mitochondria, chloroplasts)

Cell wall: sometimes present(plant cell)