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28 Cards in this Set

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The ability to distinguish two points in the specimen (i.e. see two points, instead of one) relates to a problem in using light microscopes termed
resolution
The numerical aperature of an objective lens determines the ability of that lens to ________ portions of the specimen.
discern/view
A stain is used to induce ______________ in the specimen.
contrast
The smallest element in a digital picture is refered to as a
pixel
The total magnification through a compound microscope is equal to the magnification of the ___________ times the magnification of the _________________.
objective lens; eyepiece
Oil immersion improves light microscopy by increasing the ______ of the medium between the objective lens and the specimen.
refractive index
____________________ refers to the ability to distinguish objects on each side of the plane of focus
magnification
A microscopic technique used to increase contrast in an unstained specimen is ________________.
dark field illumination
______________ is the stage of mitosis in which the chromatin material begins to condense into individual chromosomes and the nuclear membrane begins to disappear.
Prophase
______ is the stage of mitosis when the sister chromatids begin to separate to opposite poles
Anaphase
_______ is the stage of mitosis in which cytokinesis, the separation of the cytoplasm to the two daughter cells, occurs
Telophase
The objective lenses are held in a structure on the microscope known as the ___________.
revolving nosepiece
The protozoan you will study in lab this week is ____________
elodea
The source of the tissue you will use to study mitosis is ____________________
onion
The specimen to be examined with a microscope is placed on what part of the microscope? ______
stage
In the lab you will make scrappings of your __________ to look at epithelial cells.
cheek
The stain aceto-orcein is used to stain what material inside of cells? __
DNA
The lens system located between the specimen and the ocular lens is known as the _____________.
condenser lens
What power of objective lens is usually used with oil immersion? _____
100X
Scopes with a single eyepiece said to be monocular. What are scopes with two eyepieces said to be? ________
binocular
Which lens is likely to have the largest (longest) working distance? The 10X objective or the 100X objective? ________
10X
The HCl (hydrochloric acid) is used to break down the ____________ of the cells used to study mitosis.
membrane
To view the specimen on the microscope, the observer looks through the ___________ lens
objective
When you focus your microscope, you change the distance between the objective and the _______
specimen
The symbol lambda in Abbe?s equation refers to the ______________ of light
wavelength
A difference in the absorption of light by different portions of the specimen is the basis for ___________ (choose ?brightfield? or ?phase?) microscopy
brightfield
The illuminator for the microscope is located in the part of the microscope known as the _______
base
The angle theta in Abbe?s equation is _______ of the angle of the cone of light entering the microscope objective
1/2