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28 Cards in this Set
- Front
- Back
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The ability to distinguish two points in the specimen (i.e. see two points, instead of one) relates to a problem in using light microscopes termed
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resolution
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The numerical aperature of an objective lens determines the ability of that lens to ________ portions of the specimen.
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discern/view
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A stain is used to induce ______________ in the specimen.
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contrast
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The smallest element in a digital picture is refered to as a
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pixel
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The total magnification through a compound microscope is equal to the magnification of the ___________ times the magnification of the _________________.
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objective lens; eyepiece
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Oil immersion improves light microscopy by increasing the ______ of the medium between the objective lens and the specimen.
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refractive index
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____________________ refers to the ability to distinguish objects on each side of the plane of focus
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magnification
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A microscopic technique used to increase contrast in an unstained specimen is ________________.
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dark field illumination
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______________ is the stage of mitosis in which the chromatin material begins to condense into individual chromosomes and the nuclear membrane begins to disappear.
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Prophase
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______ is the stage of mitosis when the sister chromatids begin to separate to opposite poles
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Anaphase
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_______ is the stage of mitosis in which cytokinesis, the separation of the cytoplasm to the two daughter cells, occurs
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Telophase
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The objective lenses are held in a structure on the microscope known as the ___________.
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revolving nosepiece
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The protozoan you will study in lab this week is ____________
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elodea
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The source of the tissue you will use to study mitosis is ____________________
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onion
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The specimen to be examined with a microscope is placed on what part of the microscope? ______
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stage
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In the lab you will make scrappings of your __________ to look at epithelial cells.
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cheek
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The stain aceto-orcein is used to stain what material inside of cells? __
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DNA
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The lens system located between the specimen and the ocular lens is known as the _____________.
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condenser lens
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What power of objective lens is usually used with oil immersion? _____
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100X
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Scopes with a single eyepiece said to be monocular. What are scopes with two eyepieces said to be? ________
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binocular
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Which lens is likely to have the largest (longest) working distance? The 10X objective or the 100X objective? ________
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10X
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The HCl (hydrochloric acid) is used to break down the ____________ of the cells used to study mitosis.
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membrane
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To view the specimen on the microscope, the observer looks through the ___________ lens
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objective
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When you focus your microscope, you change the distance between the objective and the _______
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specimen
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The symbol lambda in Abbe?s equation refers to the ______________ of light
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wavelength
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A difference in the absorption of light by different portions of the specimen is the basis for ___________ (choose ?brightfield? or ?phase?) microscopy
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brightfield
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The illuminator for the microscope is located in the part of the microscope known as the _______
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base
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The angle theta in Abbe?s equation is _______ of the angle of the cone of light entering the microscope objective
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1/2
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