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40 Cards in this Set
- Front
- Back
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Formin
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(A)
Nucleates actin at plus end Promotes profilin |
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Profilin
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(A) promottes addition of actin monomers at plus end and competes with thymosin
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Thymosin
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(A) sequesters Actin monomers and competes with profillin
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Stathmin
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(M) Sequesters 2 free tubulin dimers, inhibited by phosphorylation
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ARP complex
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(A) Nucleates assembly and can attach at already formed actin filaments.
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Cofillin
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(A) Binds to ADP-actin filaments and accelerates dissasembly by causing increased twist which makes it more vulnerable to severing
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Gelsolin
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(A) Severs filaments and binds to plus end.
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Tropomysin
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(A) Stabilizes filament on the sides and binds a several actin
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Fimbrin
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(A) attaches parallel filaments doesnt allow myosin 2.
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alpha-actinin
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(A) attaches parallel filaments allows mysoin 2
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Filamin
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(A) forms gel like webs by attching 2 perpendicular filaments.
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Spectrin
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(A) Attaches to plasma membrane
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Kinesin 13
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(M) enhances disassembly at plus end
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Katanin
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(M) severs microtubles
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(TIPs
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(M) remains associated with growing plus end and can link them to other structures such as membranes
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MAPs
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(M) stabalizes tubules by binding along sides
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Tau
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(M) bundles tubules
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MAP-2
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(M) Bundles tubules
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Mor1
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(M) 21c stabilizes
29c destabilizes through regulation of catastrophe factors |
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Latrunculin
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(A) -ve. cell permeant binds with monomeric actin. inhibits filament polymerization
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Swinholide
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(A) -ve. Stabilizes actin dimers. severs actin filaments.
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Phalloidin
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(A) +ve binds to actin filmanets at junctions. Stabilizes filamints. competes with coffilin.
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cytochalasins
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(A) +ve. binds to plus end of filaments. Inhibits polymerization and depolymerization.
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Colchicine
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(M) -ve. binds to B-tubulin. stops assembly
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Taxol
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(M) +ve binds to B-tubulin and stabalizes microtubules. alters dynamics and structure
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Vinca alkaloids
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(M) -ve. Binds to dimeric tubulin ends. concentration dependednt. Speficifcally binds to B-tubulin and inhibits polymerization.
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Dinitroaniline
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(M) -ve Binds to N loops of a-tubulin. disrupts adjacent a-a bond.
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Who is the greatest of them all?
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Steven Pi
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Who is the hottest of them all?
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Steven Pi
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Who is the gayest of them all?
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Omar Virani
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Which myosin moves toward the negative end?
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Myosin VI
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What motor protein is a catastrophe factor?
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Kinesin 13
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Which kinesin walks toward minus end?
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Kinesin 14
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Which motor protein is the largest and fastest?
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Dyneins
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True or false: All dyneins move toward minus end
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True
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Which two motor proteins have most likely shared a common ancestry?
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Kinesins and Myosins. Central core is similar to Ras
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Steps for force generation for: Myosin
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ATP binding causes disassociation of myosin head (lever), phosphate released first and dissociation of ADP which causes power stroke
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What are the steps of force generation for kinesins?
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ATP binding causes association of motor head (linker) to microtubules.
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What are the steps of force generation for dyneins?
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ATP binding causes dissociation of dynein head (ring and stalk). However, Pi and ADP are released at the same time, causing the conformational change driving the power stroke.
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What are the 4 things that the velocity of the motor depends on.
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1. Intrinsic ATPase rate
2. Time spent bound to track (relative processivity) 3. Lever arm (myosin), linker (kinesin), or stalk (dynein length) 4. Angle through which helix (tail domain) swings. |
