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42 Cards in this Set
- Front
- Back
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What cannot grow at temps above 20 degrees C?
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obligate psychrophile
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What cannot grow at temps below 40 degrees Celcius?
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obligate thermophiles
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What grow at 37 degrees C
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facultative thermophiles
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What grow best at above 80 degrees C?
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extreme thermophiles
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What is the reducing agent for cultivation of anaerobes?
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Na thioglyollate
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WHat does NA thioglycollate do?
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chemically binds to dissolved O2 and reduces OR potential
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What is the OR indicator?
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resazurine (or methylene blue)
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What does an OR indicator do?
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changes color in response to oxidation/reduction potential in a medium
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What is the oxidized form of resazurin?
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pink
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What is the oxidized form of methylene blue?
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blue
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What is the oxidized form of litmus?
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pink-blue
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What will occur if you shake the anaerobe cultivation?
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O2 is incoporated into the broth and the broth turns pink
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Review aerobe, microaerophile, obligate aerophile, aertolerant anaerobe, facultative anaerobe
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pages 167-168
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What does the thioglycollate broth show?
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mos growth
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What occurs in a gaspak when 10 mL H20 are added?
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H2 and CO2 will be released
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What does a palladium catalyst do in a biobag?
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catalyzes a rxn and removes O2 in the jar
(2H2 + O2 -> 2 H2O) |
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What happens to the OR indicator when O2 is removed from the biobag?
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resazurin (or methylene blue) becomes colorless
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What is an enclosed chamber filled with inert gas such as N2 and CO2?
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anaerobic chamber
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What is an air tight jar that is vacuumed first then filled with inert gas?
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brewer jar
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Can a candle jar be used to cultivate anaerobes?
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no
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How much oxygen is in the jar when the lighted candle is off?
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about 16% O2 to support growth of aerobes
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What does CO2 help grow?
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capnophiles
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WHat is the lowest temp at which growth will occur?
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minimum growth temperature
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WHat is the highest temp at which growth will occur?
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maximum growth temperature
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What is the temp at which the rate of reproduction is most rapid
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optimum growth temperature
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What are a bacterial species that will grow from -5 to 20 degrees C?
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psychrophiles
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What are bacterial species that grow within a temperature range of 20 to 45 degrees C?
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mesophiles
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What grow at human body temperature?
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37 degrees C
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What are mesophiles with opt growth of 20 to 30 degrees C?
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plant saprophytes
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What are mesophiles whose opt growth temp from 35 to 40 degrees C?
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warm blooded hosts
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What are bacterial species that will grow at 35 degrees C and above?
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thermophiles
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What are thermophiles that grow at 37 degrees C with an opt growth temp of 45 to 60 degrees C?
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facultative thermophiles
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What are mos that will grow only at temps above 50 degrees C with an opt growth temp above 60 degrees C?
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obligate thermophiles
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What are the 3 things you use in an anaerobic jar/biobag?
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gaspak, palladium catalyst, OR indicator
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What require the presence of atmospheric O2 for growth?
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aerobes
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What are mos that require limited amounts of atmospheric oxygen for growth but excess o2 blocks the activities of oxidative enzymes and results in death?
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microaerophiles
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What require the absence of free oxygen for growth?
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obligate anaerobes
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What does O2 do in obligate anaerobes?
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presence of atmospheric oxygen results in the formation of toxic metabolic end products
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What are mos that donot use O2 but they are not killed by it either?
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aerotolerant anaerobes
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What are mos that prefer to use oxygen for aerobic respiration however they can survive without oxygen?
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facultative anaerobes
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How can you determine the oxygen needs of mos?
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noting their growth distributions following a shake tube inoculation
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What are the devices used to cultivate anaerobes?
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biobag, anaerobic chamber, brewer jar, candle jar (not true ones)
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