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32 Cards in this Set

  • Front
  • Back

Soft deposits

Acquired pellicle


Dental biofilm


Materia alba


Food debris


Acquired pellicle

Thin acellular, tenacious film formed of protein, carbs and lipids


Positioned between tooth and oral environment. Also over exposed tooth surface and protheses.


Thickest near gingiva margin.

Acquired pellicle

Supra gingival and subgingival in crevicular fluid.


Can remove with tooth brush.

How fast does a pellicle form.

Fully formed within 30-90 min.


Composition glycoproteins that are selectively absorbed by hydroxyapitite of tooth surface. Proteins derived from saliva, oral mucosal cells, GCF, and microorganisms.

How many types of pellicle are there

2


Supra and sub ginguval.

Supragingival

Clear, translucent, insuluble


Not visible until disclosing tablet is applied. Can appeared stained.

Subgingival

Continuous with supragingival pellicle becomes embedded in tooth structure especially on demineralized surfaces or rough surfaces caused by iatrogenic dentistry.

Significance of pellicle

Protection


lubrication


Nidus for bacteria (provides method of adherence of microorganisms)


Attachment of calc

Dental biofilm

Dynamic structured community of microorganisms encapsulated in a self-produced extracellular polymeric substance for a matrix around micro colonies.

EpS

Extracellular polymeric substance like a glue that holds everything g together and makes it harder to remove

How can you remove biofilm

Good home care usually but not always.

Matrix

Polysaccharides and proteins


Protects biofilm from hosts immune response.


, adapt and respond to enviroment.


3D helps it adapt and communicate, adapt and respond to enviroment.Biofilm adheres to living moist surfaces(tongue tonsils, pellicle)700 distinct bacteria


Biofilm adheres to living moist surfaces(tongue tonsils, pellicle)


(tongue tonsils, pellicle)700 distinct bacteria


700 distinct bacteria

5 stages of biofilm

Formation


Bacteria multiplication and colonization


Matrix formation


Biofilm growth


Maturation

Formation

Begins with the initial attachment of planktonic bacterial cells to pellicle on tooth surface by way of interaction with pellicle


Reversible-cells can be disrupted


May resume planktonic life and either begin formation somewhere else.

Bacteria multiplication and colonization

If not removed the planktonic microorganisms will attach themselves permanently using fimbrea, pili, flagella, and adhesion protiens.


Grow in layers up and out


3d


Use EPS so they are hard to remove.

Attachment is quiker?

On rough surfaces.

Matrix formation

Bacteria continues to secrete EPS as they form.


EPS anchors bacteria together increasing adherence to teeth.


Resists weak oral hygiene. And antimicrobial therapy

Biofilm growth

Characterized by further development of biofilm architecture that enhances cell to cell communication. Activated by specific genes located on surface of bacteria cells within biofilm called quarum sensing

Maturation

Bacteria colonies mature and release planktonic cells to spread and colonize other areas in the oral cavity.


Bacteria can disperse in clumps and single cells.

Gingivitis

Develops in 2-3 weeks when biofilm is left undisturbed on tooth surface.


It is reversible. Gingiva healthy again in a few days

Subgingival and supragingival biofilm

Subgingival biofilm results from supragingival biofilm


Supragingival biofilm proliferates apically.


Subgingival biofilm may invade connective tissue.

Composition of biofilm

80% water


20% EPS and microorganisms both inorganic and organic elements.

Inorganic elements

Calcium and phosphorus and floride(gets into the biofilm and pellicle)

Organic elements

Carbs(sticky) adds to biofilm tenacity.


Proteins(originate from gingival crevicular fluid.

Detection of biofilm

Direct vision.


Explorer or probe


Disclosing agent


Clinical record(location, thickness,biofilm, scores)

Biofilm

Plays a major role in ignition and progression of dental caries and disease. Formation of calc. Inpleadent malod9r and appearance.

Dental caries

Disease of dental calcified structures characterized by deminerilization of mineral components and dissolution of organic matrix.

PH of biofilm

Acid formation begins immediately when carcinogenic substance is taken into biofilm.


Biofilm ph lowers quickly.


Critical ph level for demin is 4.5-5.5


Root surface demin happens at 6-6.7ph

Distribution of biofilm

Location= supragingival, gingival, fissure,subgingival.


Heaviest at gingival third and interpoximal spaces.


Influencing factors: crowding teeth, rough surface, occlusion.

Materia alba

White material cottage cheese texture and appearance.


Composed of food debris and dead cells .


Contributes to inflammation can cover demin and materia alba

Food debris

Collects at cervical 3rd and embrassure. Food impaction

Documentation

Clinical description


Client understanding


Clients description of methods to remove biofilm.