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13 Cards in this Set
- Front
- Back
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What is ATP?
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Adenosine nucleoside with three phosphate groups attached to the 5' carbon on the sugar
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What are nucleotides attached together by?
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phosphodiester bond
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What is a nucleic acid?
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long chain of nucleotides
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In what direction is a phosphodiester bond made?
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It takes the 3' OH from the existing sugar and binds it to the 5' phosphate group of the incoming sugar.
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How do we read DNA or RNA?
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5' to 3'
At the beginning of DNA there is a loose phosphae group because there is no previous nucleotide's OH to bind to. |
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By convention, in which direction is nucleotide order?
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5' to 3'
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What base always binds with Thymine?
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Adenine
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Which base always binds with cystine?
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guanine
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What are the bonds between AT and GC nucleotides known as and how many bonds are there between the respective groups?
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There are two hydrogen bonds between A and T.
There are three hydrogen bonds between C and G |
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What type of DNA is most of our DNA?
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B-DNA
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What is the significance of the major and minor grooves?
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they act as receptors for DNA Binding Proteins, which regulate the transcription of DNA
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How do we measure the denaturation of DNA when it is being heated?
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Measure the absorbance of light. As it dissosiates it will absorb more light because the base pairs are showing and absorbing the light.
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how is the melting point of a strand of DNA determined.
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It is the temperature at which half of the DNA is denatured.
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