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13 Cards in this Set

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What is ATP?
Adenosine nucleoside with three phosphate groups attached to the 5' carbon on the sugar
What are nucleotides attached together by?
phosphodiester bond
What is a nucleic acid?
long chain of nucleotides
In what direction is a phosphodiester bond made?
It takes the 3' OH from the existing sugar and binds it to the 5' phosphate group of the incoming sugar.
How do we read DNA or RNA?
5' to 3'
At the beginning of DNA there is a loose phosphae group because there is no previous nucleotide's OH to bind to.
By convention, in which direction is nucleotide order?
5' to 3'
What base always binds with Thymine?
Adenine
Which base always binds with cystine?
guanine
What are the bonds between AT and GC nucleotides known as and how many bonds are there between the respective groups?
There are two hydrogen bonds between A and T.
There are three hydrogen bonds between C and G
What type of DNA is most of our DNA?
B-DNA
What is the significance of the major and minor grooves?
they act as receptors for DNA Binding Proteins, which regulate the transcription of DNA
How do we measure the denaturation of DNA when it is being heated?
Measure the absorbance of light. As it dissosiates it will absorb more light because the base pairs are showing and absorbing the light.
how is the melting point of a strand of DNA determined.
It is the temperature at which half of the DNA is denatured.