Biochemistry Szweda

Front 1

What is the carbon carrier between glycolysis and the mitochondria?*

Back 1

pyruvate

Front 2

Pyruvate is converted to Acetyl CoA with what enzyme? What co-factor does this enzyme use?*

Back 2

pyruvate dehydrogenase, TPP

Front 3

Which forms C-C bonds from CO2, biotin or TPP?*

Back 3

biotin

Front 4

What 1 thing is consumed in each turn of the Krebs cycle?*

Back 4

Acetyl CoA

Front 5

What is the name for the type of reaction that replenishes the Krebs Cycle?*

Back 5

Anapleurosis

Front 6

What cofactor is used in pyruvate carboxylase?*

Back 6

biotin

Front 7

The Krebs cycle creates what 4 things and how many of each?* (3 are very important)

Back 7

3 NADH, 1 GTP (ATP), 1 FADH2, 2 CO2

Front 8

What is the order of the ETC starting at complex 1? At complex 2?*

Back 8

1 > ubiquinine > 3 > cyt. C > 4

2 > ubiquinine > 3 > cyt. C > 4

Front 9

Complex 3 reduces what?*

Back 9

cytochrome C

Front 10

In ATP synthase, which part is exposed and which part turns using the proton motive force?*

Back 10

F1, F0

Front 11

What type of steps are the best to regulate?*

Back 11

Those that are thermodynamically FAVORABLE

Front 12

What 3 enzymes in Krebs are highly regulated?* (Bonus: WHY are these good to regulate?)

Back 12

citrate synthase, isocitrate dehydrogenase, alpha-ketogluterate dehydrogenase (Bonus: bc they are thermodynamically favorable)

Front 13

When ATP levels are high, Krebs cycle is (low or high?)*

Back 13

low

Front 14

NADH (inhibits, activates) the Krebs cycle*

Back 14

inhibits

Front 15

Krebs Cycle takes place where?*

Back 15

The Matrix of the mitochondria

Front 16

What enzyme is associated with both Krebs and the ETC?*

Back 16

Complex 2 is also succinate dehydrogenase

Front 17

NADH goes to which complex in ETC?*

Back 17

1

Front 18

FADH2 goes to which complex in ETC?*

Back 18

2 (Think... FADH2 goes to 2... 2 and 2!)

Front 19

What is reduced by both complex 1 AND complex 2?*

Back 19

ubiquinone

Front 20

What are the two mobile carriers in ETC?*

Back 20

ubiquinone and cytochrome C

Front 21

oxygen consumption (increases, decreases) upon production of ATP*

Back 21

increases

Front 22

Breaking the proton gradient in the ETC (increases, decreases) O2 consumption and (increases, decreases) flux through Krebs*

Back 22

increases, increases

Front 23

If you add an uncoupler, what 3 things happen?*

Back 23

1. O2 consumption goes up
2. ATP production goes down
3. you give off more heat

(This is because an uncoupler 'uncouples' electron transport from ATP synthesis)