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14 Cards in this Set

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  • Back
describe the fate of pyruvate under anaerobic
forms lactate thru the lactate dehydogenase enzyme
Explain the role of thiamine pyrophosphate(TPP)
Its a coenzyme derived from vit B1 that carries "Active Acetoaldehyde" groups

It aids in ethanol fermentation, synthesis of acetyl-CoA in citric cycle and aids in carbon assimilation rxns of the pentos phosphate pathway
what can a vit B1 deficiency cause
describe the ethanol fermentaion process
Pyruvate is first converted to Acetylaldehyde thru pyruvate decarboxylase enz along with TPP and Mg2+ as "coenzymes" and CO2 is given off. Then acetaldehyde is converted to ethanol thru alcohol dehydrogenase by oxidation of NADH+ + H+ to NAD+
how many grams of glucose does the brain require per day
120, but only 60g can be stored in our body
T or F Gluconeogensis is the exact reverse of glycolysis
F, due to large -delta G of 3 steps in glycolysis
give the relationship between Keq and Delta G
Keq > 1.0 then G is negative rxn goes fwd
Keq = 1.0 then G is zero rxn in equil.
Keq < 1.0 then G is positive rxn goes in reverse
T or F all enzymes used in glycolitic pathway are found in the mitoch
F, they are all found in cytosol
T or F all enzymes used in gluconeogenesis are found in the cytosol
F, 2 of them are found in mitchondria
What are the 3 bypass reactions in gluconeogenesis
1 - pyruvate converted to phosphoenolpyruvate thru pyruvate carboxylase enzyme found in mitochondria
2 - Conversion of fructose 1,6 Bisphosphate to Fructose 6-phosphate
3 - glucose 6-phosphate converted to glucose
What substrate is a potent regulator of both glycolysis and gluconeogenesis
Fructose 2,6-Bisphosphate

lots of above molecule leads to activation of glycolysis
describe the 2 branches of pentose phosphate pathway
Oxidative phase- production of NADPH

Non oxidative phase-
What is the role of NADPH in partioning glucose 6-phosphate between glycolysis and pentose phosphate pathway
as you push oxidative path of ribose phosphate pathway lots of NADPH is produced which inhibits the pathway- i get negative feedback from this but it wasnt stated in lecture, he covered it shitily
of the two enzymes transketolase and transaldolase which one uses TPP to proess its substrates