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8 Cards in this Set

  • Front
  • Back
Agarose gel eletrophoresis separates DNA fragments based on what?
Size - smaller travels further
What is labeled on a Southern blot?
DNA fragments
What is labeled on a Northern blot?
RNA fragments
What is labeled on a Western blot?
Proteins
What is labeled on a Southwestern blot?
DNA-binding proteins (e.g. transcription factors)
How does ELISA work?
Patient blood is probed with a test antigen or test antibody coupled to a color-generating enzyme activated if the reactive antigen or antibody is present
What is the cre-lox system?
Inducibly manipulates genes at developmental points with antibiotic-controlled promotor

Allows study of genes whose deletion --> death
What is RNAi?
dsRNA that is complementary to the target mRNA sequence; transfection into cells promotes degradation of the target mRNA, lowering gene expression