Biochemistry Jkjkhardcore

Front 1

Step 1 enzyme

Back 1

Hexokinase
Requires ATP coupled
Irreversible

Front 2

Step 1 Reasctant and product

Back 2

Glucose and Glucose 6 phosphate

Front 3

Step 2 enzyme

Back 3

Phsphoglucose Isomerase

Front 4

Step 2 Reactant and product

Back 4

Glucose 6 phosphate
Fuctorse 6 phosphate

Front 5

Step 3 enzyme

Back 5

Phosphofuctokinase (PFK-1)

Front 6

Step 3 reactant and products

Back 6

Fuctose 6 phosphate to
Fructose 1,6 bisphosphate

Front 7

step 4 enzyme

Back 7

aldolase

Front 8

Step 4 reactant and products

Back 8

Fructose 1,6 biphosphate makes
GA3P and DHAP
Gluceraldehyde 3 phosphate and Dihydroxyacetone Phosphate

Front 9

Step 5 enzyme

Back 9

Triose Phosphate isomaerase (TIM)

Front 10

Step 5 Reactant and product

Back 10

DHAP product will be GA3P

Front 11

Step 6 enzyme

Back 11

Glyceraldehyde 3 phosphate Dehydrogenase

Front 12

Step 6 reactants and products

Back 12

GA3P becomes 1,3 Bisphosphoglycerate

Front 13

Step 7 enzyme

Back 13

Phsophoglycerate kinase

Front 14

Step 7 reactants and products

Back 14

1,3 Bisphosphoglycerate to
3 phosphoglycerate

Front 15

Step 8 Enzyme

Back 15

Phosphoglycerate mutase

Front 16

Step 8 Reactants and products

Back 16

3 phosphoglycerate to 2 phosphoglycerate

Front 17

Step 9 enzyme

Back 17

enolase

Front 18

Step 9 reactants and products

Back 18

2 phosphoglycerate makes
PEP

Front 19

Step 10 enzmyme

Back 19

Pyruvate kinase

Front 20

Step 10 reactant and product

Back 20

Pep > Pyruvate

Front 21

Which steps are irrerversable in Glycolysis

Back 21

Steps 1, 3 and step 10

Front 22

Which steps/ reactions in glycolysis are coupled with ATP breakdown:

Back 22

1 3

Front 23

Which steps coupled with ATP synthesis

Back 23

7&10

Front 24

Which steps are coupled with Making NADH

Back 24

Step 6

Front 25

Which step has removal of water

Back 25

step 9

Front 26

Which enzyme has mg, and K+

Back 26

pyruvate kinase

Front 27

Which enzymes require Mg?

Back 27

phosphoglucose isomerase
phosphofuctokinase-1
phosphoglycerate mutase
And pyruvate kinase (but that requires K as well)

Front 28

Which enzyme requires a Thioester intermediate?

Back 28

GA3P dehydrogenase

Front 29

Which step goes through posphorolysis?

Back 29

Step 6

Front 30

Whiat enzyme is specific for Glc6p and Fct6P

Back 30

Phosphoglucose isomerase

Front 31

Which is the major regulator point in glycolysis?

Back 31

Step 3?

Front 32

Which enzyme has a perfect catalytic rate?

Back 32

TIM

Front 33

Which two Amino acids interact at Tim's active site?
How to they interact?

Back 33

GLU and HIS
they interact via acid base catalysis

Front 34

Structure of TIM?

Back 34

Alpha then Beta repeats in a barrel and an Oxyanion hole.

Front 35

What step is the redox reaction of glycolysis?

Back 35

Step 6

Front 36

Glyceraldehyd 3 phosphate dehydrogenase has 3 components in its active site. What are those 3 ?

Back 36

NAD+ (gets regenerated) His 176 cys 149

Front 37

What does phosphoglycerate kinase do in Glycolysis?

Back 37

It transfers a Phosphate to ADP from 1,3 BPG

Front 38

What does phosphoglycerate mutase do? What does it require as cofactors? What is at it's active site?

Back 38

It transfers a Phosphate group
It requres not only MG but als o 2,3BPG as cofactors. At the active site there is a a phosphorylated HIS

Front 39

Where does enolase remove water?

Back 39

At carbon 2 and 3 on 2 phosphoglycerate

Front 40

What are the two examples of substrate level phosphorylation?

Back 40

PEP and ADP to Pyr and ATP Step 10
Step 7 Phosphoglycerate kinase Making ATP
Whenever you make ATP