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30 Cards in this Set
- Front
- Back
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(DNA dependent) DNA polymerase requires:
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4 dNTP substrates
template primer divalent cation |
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T/F primer requirement shows DNA Pol catalyzing daughter strand synthesis initiates synthesis from scratch
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F
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What kind of bonds hold the primer to the template?
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hydrogen bonds
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Primers for bacterial DNA synthesis are:
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RNA oligomers
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DNA primase is a specialized functional type of:
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DNA-dependent RNA polymerase
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RNA polymerase can synthesize RNA de novo.... do they need a primer?
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no.
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Primer synthesis biochemical requirements:
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ssDNA template, rNTP's, divalent cation
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Primer synthesis: reaction is identical to DNA pol, except::
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complementary ribonucleotides are polymerized on DNA template
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Primase is located where?
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at the fork
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Primers are removed and replaced on the lagging strand by ______
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DNA Pol I
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Nick Translation
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primers are removed and replaced on the lagging strand by DNA Pol I
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RNA synthesis is usually _______
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template directed
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RNA synthesis from a DNA template is called:
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transcription
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E. Coli has approximately how many transcription units?
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3,000 genes
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rRNA function?
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ribosome
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tRNA function?
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adaptor
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mRNA function?
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protein-coding
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RNA primers function?
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DNA synthesis
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In bacteria, ______ levels control initiation of transcription.
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steady-state
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(DNA dependent) RNA polymerase transcription complex:
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(4) rNTP substrates
DNA template divalent cation |
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____-acting gene elements upstream form transcription start site
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cis
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What is numerical value given to RNA transcription start site?
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+1
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Bacterial housekeeping promoter comprise of two sequence elements centered at ___ & ___ and conserved spacing between them and +1
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-10 (TATAA)
-35 (TTGACA) |
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The functional promoter is on ____ strand(s) of DNA
one or both? |
both
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Interaction of the transcription complex with the promoter controls the _____ of transcription initiation (gene expression)
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rate (efficiency)
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Mutations can influence promoter strength- an "up promoter" mutation will make a promoter element ____ like consensus.
-a "down promoter" mutation will make a promoter element ____ like consensus |
more
less |
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RNA Pol I
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transcribes rRNA (except 5S rRNA)
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RNA Pol II
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transcription of DNA to synthesize percursors of mRNA & snRNA
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RNA Pol III
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transcribes DNA to synthesize 5S rRNA & tRNA
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Eukaryotic RNA transcription requires _____ to bind to promoter and initiate transcription
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transcription factors
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