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46 Cards in this Set
- Front
- Back
Nonpolar, non-aromatic |
Glycine, alanine, valine, leucine, isoleucine, methionine, proline |
|
Aromatic |
Tryptophan, phenylalanine, tyrosine |
|
Polar |
Serine, threonine, asparagine, glutamine, cysteine |
|
Negatively charged, acidic |
Aspartate, glutamate |
|
Positively charged, basic |
Lysine, arginine, histidine |
|
pKa |
pH where half of the molecules are deprotonated |
|
Isoelectric point |
Average of 2 pKas |
|
Peptide bond formation |
Condensation, dehydration |
|
Breaking a peptide bond |
Hydrolysis |
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Apoenzyme |
No cofactor |
|
Holoenzyme |
Contain a cofactor |
|
Cofactor |
Inorganic, metal ion |
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Coenzyme |
Organic, vitamins |
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Enzyme kinetics, v |
vmax[S]/(Km+[S]), kcat[E][S]/(Km+[S]) |
|
vmax |
[E]kcat |
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Competitive inhibition |
Same y-intercept |
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Noncompetitive inhibition |
Same x-intercept |
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Uncompetitive inhibition |
Parallel lines |
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Actin |
Thin filaments |
|
Negative end |
Nucleus |
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Positive end |
Periphery |
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Myosin |
Thick filaments |
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Kinesins |
Bring vesicles to the positive end |
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Dyneins |
Bring vesicles to the negative end |
|
Gs |
Stimulate adenylate cyclase, more cAMP |
|
Gq |
Activate phospholipase C, more calcium in the cell |
|
Migration velocity |
(electric field*net charge)/(frictional coefficient) |
|
Move faster in electrophoresis |
Small, highly charged, large electric field |
|
Positively charged protons in isoelectric focusing |
Migrate to the cathode |
|
Column chromatography |
Nonpolar compounds elute faster |
|
Size-exclusion chromatography |
Large compounds elute faster |
|
Bradford protein assay |
More protein means more blue dye |
|
Epimer |
Different at one chiral center |
|
Alpha anomer |
Axial, down |
|
Beta anomer |
Equatorial, up |
|
Sucrose |
Glucose, fructose |
|
Lactose |
Galactose, glucose |
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Maltose |
Glucose, glucose |
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Saturated fatty acid |
Solid |
|
Unsaturated fatty acid |
Liquid |
|
Sphingomyelin |
Phosphodiester bond |
|
Glycosphingolipid |
Glycosidic linkage |
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Monoterpene |
Two isoprenes |
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Sesquiterpene |
Three isoprenes |
|
Purine |
Two rings, adenine, guanine |
|
Pyrimidine |
One ring, cytosine, thymine, uracil |