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41 Cards in this Set
- Front
- Back
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what is special about glucose?
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it has a durham tube
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what do carboydrates and sugar tests test for?
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break down of glucose resulting in the release of pyruvic acid
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what is the indicator in carbohydrates and sugars?
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phenol indicator
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what are positive, negative results for carbohydrates and sugar tests and what do they mean?
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positive: yellow: acidic pH
negative: red or a reddish orange: neutral pH negative: pink: basic pH |
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what are starch tests looking for?
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starch hydrolysis: AMYLASE
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what is the reagent for starch testing?
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gram's iodine
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what are the results for starch tests?
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positive: amylase present: clearing of reagent around bacterial colony
negative: amylase not present: iodine reacts with starch causing a dark complex color around the colonies |
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what does SIM stand for?
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sulfide, motility and indole
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what does a positive motility test look like?
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cloudiness around the stab line
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what is the indicator in SIM concerning sulfide?
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metal ions
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what does a positive test for sulfide production look like?
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black
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what is indole testing for?
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the enzyme tryptophanase
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what is the reagent used in indole?
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kovac's reagent
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what do the results look like for indole?
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negative: no color change of the kovac's reagent
positive: bright color change |
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what is phenylalanine testing for?
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phenalalanine deaminase: breaks down phenalalanine in to phenol and pyruvic acid
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what is the reagent associated with pheylalanine?
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ferric chloride
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what are the results for phenylalanine?
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positive: green color change
negative: no change in color |
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what are MR-VP tests looking for?
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MR: highly acidic end products
VP: neutral acetion end products |
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what is the indicator added to MR-VP when testing for MR?
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methyl red
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what are the 2 chemicals added for voges-proskauer tests?
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alpha-napthol and KOH
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what are the results for a voges-proskauer test?
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positive: cranberry color formation
negative: "spit in coke" color change |
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what are the results for a methyl red test?
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positive: red color formation
negative: no color change |
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what is citrate looking for?
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if organism uses citrate as its sole CARBON source during metabolism
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what is the indicator for citrate?
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bromknesol blue
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what are the results for a citrate?
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positive: blue color change
negative: green color change |
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what is dnase testing for?
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DNAse
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what are results for dnase?
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positive test: clearing of methyl green color
negative test: no change in methyl green color of agar |
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what is gelatin looking for?
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gelatinase
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what are results for gelatin?
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positive: liquidy gelatin
negative: resolidifies |
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what is urea looking for?
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urease
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what is the reagent for urea?
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phenol red
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what are the results for urea?
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positive: hot pink color: basic pH
negative: yellow: acidic pH |
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what are the 2 types of decarboxylases?
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ornithine and lysine
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what are decarboxylase tests looking for?
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decarboxylase enzymes
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what must you always run along with decarbosylase testing?
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the base decarboxylase
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what must you add to decarboxylase tests to keep from false results?
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mineral oil
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what are results of decarbosylase tests?
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positive: deep purple: basic pH
negative: yellow: acidic pH |
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what color should the base decarboxylase always turn out as?
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yellow
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what is catalase looking for?
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catalase: breaks down hydrogen peroxide
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what are results for catalase?
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positive: bubbles
negative: no bubbles |
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what do you need to run a catalase test?
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hydrogen peroxide and a sterile wooden stick
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