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42 Cards in this Set

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  • Back
what influences how protein folds?
1) thermodynamics- determines if it is energetically favored
2) kinetics - determines how fast follding happens
3) protein fold is not random
what is a molten globule
when molecule has folded into discrete secondary regions.
what is the name of strucutre after it folds into tertiary structure
native structure
what is Ks equal to
k-1/ k1
what two ways can an enzyme promote a rxn
1) can destabalize the substrate
2) can bind to transition state and lower energy/ stabalize them
Does Vmax change in competitive inhibition?
no because u can add enough substrate to outcompete the inhibitor.
draw graph for what changing concentration of inhibitor in competitive inhibition affects teh line Weaver Burke graph
p 422
In what type of inhibition is Vmax not affected by [I]?
competitive p422
if Ki does not equal Ki' what type of inhibition is that
mixed non competitive
what amino acids does chymotripsin cleave after
aromatic ones- phenylalanine, tyrosine, trptophan.
what methods to enzymes use to drive foward reaction
1) proximetry
2) electrostatics - chymotripsin
3) covalent bonding-bacterial cell wall
4) acid/ base - chymotripsin
what do proteases do?
hydrolize proteins
what type of protease uses a metal ion in order to make a protein reactive?
metallo protesase p452
what type of enzyme is HIV protease and what are the two amino acids in its active site
asparteal protease (homodimer)/ aspartic acids
what methods to enzymes use to drive foward reaction
1) proximetry
2) electrostatics - chymotripsin
3) covalent bonding-bacterial cell wall
4) acid/ base - chymotripsin
what do proteases do?
hydrolize proteins
what type of protease uses a metal ion in order to make a protein reactive?
metallo protesase p452
what type of enzyme is HIV protease and what are the two amino acids in its active site
asparteal protease (homodimer)/ aspartic acids
what methods to enzymes use to drive foward reaction
1) proximetry
2) electrostatics - chymotripsin
3) covalent bonding-bacterial cell wall
4) acid/ base - chymotripsin
what do proteases do?
hydrolize proteins
what type of protease uses a metal ion in order to make a protein reactive?
metallo protesase p452
what type of enzyme is HIV protease and what are the two amino acids in its active site
asparteal protease (homodimer)/ aspartic acids
what are the 6 ways that enzymes are regulated
1) product inhibition- product of reaction builds up
2) substrate avaliability
3) genetic mechanisms
4) covalent modification- phosphorylation
5) zymogen- enzyme synthasized in inactive form
6) allosteric regulation- product in pathway will affect an earlier enzyme- pos/ neg feedback
what is the region of RNA where product can interact with it and cut off the expression
riboswitches
none competitive
Vmax, Km
competitive
Vmax, alphaKm
mixed non competitive
Vmax/ alpha' ,

(alphaKm)/alpha'
uncompetetive
Vmax/alpha', Km/alpha'
pure noncompetitive
Km, Vmax/ alpha
none competitive
Vmax, Km
competitive
Vmax, alphaKm
mixed non competitive
Vmax/ alpha' ,

(alphaKm)/alpha'
uncompetetive
Vmax/alpha', Km/alpha'
competitive
Vmax, Km
competitive
Vmax, alphaKm
mixed non competitive
Vmax/ alpha' ,

(alphaKm)/alpha'
uncompetetive
Vmax/alpha', Km/alpha'
none competitive
Vmax, Km
competitive
Vmax, alphaKm
mixed non competitive
Vmax/ alpha' ,

(alphaKm)/alpha'
uncompetetive
Vmax/alpha', Km/alpha'
draw out mechanism for lysozyme reacting with sugar
p 468