Biochem Recombinant Dna Iv

Front 1

into which part of developing mouse is gene of interest injected in the creation of transgenic mice?

Back 1

pronucleus

Front 2

when creating a transgenic mouse, you must pay particular attention to what?

Back 2

the promoter being used and possible consequences of expression of the transgene

Front 3

which two factors are important to integrate into the cloned gene for selection of genes used in creation of knockout mice?

Back 3

antibacterial resistance for positive selection and thymidine kinase for negative selection

Front 4

what does negative selection prevent in creation of the construct for knockout mice?

Back 4

the construct adding at the improper location -- non-homologous recombination

Front 5

by what process is a host gene replaced by a mutated gene construct in the creation of knockout mice?

Back 5

homologous recombination

Front 6

what phenotype is used in mice to select mice heterozygous for the knockout mutation?

Back 6

fur color

Front 7

into which embryonic structure are stem cells mutant for the selected gene injected in the creation of knockout mice?

Back 7

blastocoel

Front 8

if you breed a chimeric mouse with a homozygous wildtype mouse with skin the color of the foster mother, the heterozygous mice that result will have what makeup for the gene of interest?

Back 8

X+/X+ or X+/X-

Front 9

which genotype for the gene of interest is selected and bred to form a homozygous knockout mouse?

Back 9

X+/X-