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19 Cards in this Set
- Front
- Back
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what is the most commonly used technique to sequence DNA?
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Sanger-dideoxy chain terminating method
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why do ddNTP's stop a polynucleotide chain?
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because they lack the 2' OH to form a subsequent phodiester linkage
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what is done in Sanger DNA sequencing to assure that chain termination does not occur too frequently?
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ddNTPs are only added at very low concentrations
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how is each reaction different in the Sanger DNA sequencing technique?
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each reaction has a different ddNTP
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following the termination of 4 reactions for Sanger DNA sequencing technique, what must be done?
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DNA must be denatured and run on a polyacrilamide gel
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while DNA sequencing was at one time visualized using primers tagged with radioactive phosphate, what technique is most commonly used now?
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fluorescent tagging of nucleotides
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other than the nature of the tagging, what is a major difference between fluorescent and radioactive DNA sequencing?
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fluorescent is done in a single tube
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where are the most common sites of DNA polymorphisms?
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junk DNA or introns
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what is the most essential difference between Northern and Southern blot techniques?
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Southern blot uses DNA while Northern blot used RNA
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what is the nature of the probe in Southern blots?
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DNA probe
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which type of mutations can a probe detect in Southern blots?
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mutations that affect the restriction site of restriction enzymes
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what is the purpose of PCR?
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to amplify very minute quantities of DNA
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what is the first step of PCR?
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denature DNA
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what special characteristic of DNA polymerase must be true for PCR?
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heat-tolerant
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where are primers placed in the performance of PCR?
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on each strand of DNA framing the desired gene.
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which aspect of microsatellite repeats varied from person to person?
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the number of copies of di- or tri-nucleotide repeats
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for which sequences would one create primers for PCR in identification of microsatellite repeats?
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the sequences which flank the microsatellite regions
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what is the name for the process by which DNA is analyzed by counting the microsatellite repeats?
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DNA fingerprinting
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other than Souther blots, which technique is useful in detecting single nucleotide polymorphisms?
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PCR and hybridization with probes which represent different SNP sequences
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