Study your flashcards anywhere!

Download the official Cram app for free >

  • Shuffle
    Toggle On
    Toggle Off
  • Alphabetize
    Toggle On
    Toggle Off
  • Front First
    Toggle On
    Toggle Off
  • Both Sides
    Toggle On
    Toggle Off
  • Read
    Toggle On
    Toggle Off

How to study your flashcards.

Right/Left arrow keys: Navigate between flashcards.right arrow keyleft arrow key

Up/Down arrow keys: Flip the card between the front and back.down keyup key

H key: Show hint (3rd side).h key

A key: Read text to speech.a key


Play button


Play button




Click to flip

19 Cards in this Set

  • Front
  • Back
what is the most commonly used technique to sequence DNA?
Sanger-dideoxy chain terminating method
why do ddNTP's stop a polynucleotide chain?
because they lack the 2' OH to form a subsequent phodiester linkage
what is done in Sanger DNA sequencing to assure that chain termination does not occur too frequently?
ddNTPs are only added at very low concentrations
how is each reaction different in the Sanger DNA sequencing technique?
each reaction has a different ddNTP
following the termination of 4 reactions for Sanger DNA sequencing technique, what must be done?
DNA must be denatured and run on a polyacrilamide gel
while DNA sequencing was at one time visualized using primers tagged with radioactive phosphate, what technique is most commonly used now?
fluorescent tagging of nucleotides
other than the nature of the tagging, what is a major difference between fluorescent and radioactive DNA sequencing?
fluorescent is done in a single tube
where are the most common sites of DNA polymorphisms?
junk DNA or introns
what is the most essential difference between Northern and Southern blot techniques?
Southern blot uses DNA while Northern blot used RNA
what is the nature of the probe in Southern blots?
DNA probe
which type of mutations can a probe detect in Southern blots?
mutations that affect the restriction site of restriction enzymes
what is the purpose of PCR?
to amplify very minute quantities of DNA
what is the first step of PCR?
denature DNA
what special characteristic of DNA polymerase must be true for PCR?
where are primers placed in the performance of PCR?
on each strand of DNA framing the desired gene.
which aspect of microsatellite repeats varied from person to person?
the number of copies of di- or tri-nucleotide repeats
for which sequences would one create primers for PCR in identification of microsatellite repeats?
the sequences which flank the microsatellite regions
what is the name for the process by which DNA is analyzed by counting the microsatellite repeats?
DNA fingerprinting
other than Souther blots, which technique is useful in detecting single nucleotide polymorphisms?
PCR and hybridization with probes which represent different SNP sequences