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5 Cards in this Set
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for glycolysis know starting material, products, where ATP and NAD+ are generated
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starting material: glucose
products: 4 ATP per glucose (2 per GAP), one produced at conversion of 1,3bisphosglycerate to 3-phosglycerate and from PRP to pyruvate via pyruvate kinase NAD+ is generated (to regenerate NAD+ that was turned into NADH) via shuttling into mito or pyruvate to LActate |
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know irreversible steps of glycolysis:
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1st step, P of glucose via HEXOKINASE, cosubstrate ATP-Mg
pep to pyruvate via pyruvate kinase F6p to F1,6P via PFK1 |
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difference between an isomerase and a mutase
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mutase shifts a chemical group from one position to another within the same molecule
isomerase gives say glucose to fructose |
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two examples of substrate level phosphorylation seen in glucose
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one produced at conversion of 1,3bisphosglycerate to 3-phosglycerate via 3phosphoglycerate kinase
and from PRP to pyruvate via pyruvate kinase |
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understand the mechanism of glyceraldehyde-3-phosphate dehydrogenase
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the first of the two substrate level phosphorylation occuring in glycolysis, works in conjunction with 3-phosphoglycerate kinase
uses NAD common intermediate: 1,3bisphosphoglycerate |