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14 Cards in this Set
- Front
- Back
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What is the fourth type of RNA? |
Small or non-coding RNAs |
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T/F: There is more RNA than DNA in the cell, and RNA can actually be catalytic. |
True |
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In basic solutions, what happens to DNA? RNA? |
DNA is converted to its tautomeric form; RNA is cleaved |
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Name the 3 enzymes that cleave RNA and where they cleave (always 3'). |
RNase A (3' to pyrimidines) RNase T1 (3' to G) RNase U2 (3' to purines) |
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What two enzymes are involved in the processing of pre-rRNA transcripts? |
RNase P and III |
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The 5' terminus of tRNA is created by... |
RNase P |
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Discuss the major structure of tRNA. |
The acceptor stem has a CCA sequence that attaches to the amino acid; the anticodon loop consists of 7 nts, with the middle 3 being the anticodon |
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What does the bulky modification involving the purine 3' of the anticodon do? |
Eliminates wobble involving the first position of the codon in the mRNA. |
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Name the major structures of tRNA, starting with the acceptor stem, going counter-clockwise. |
Acceptor stem, D loop, anticodon loop, variable loop, TWC loop |
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What is the tertiary structure of tRNA? |
L-shaped tertiary structure |
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T/F: The tRNA structure includes 9 tertiary contacts between the two helical stacks; 8 of these interactions are non W-C pairings. |
True |
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tRNAs that accept the same amino acid are called... |
Isoacceptors |
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Name the overall equation for the aminoacylation of tRNA and the enzyme that catalyzes the reaction. |
Rxn catalyzed by aminoacyl-tRNA synthetases: aa + tRNA + ATP --> aa-tRNA + AMP + PP(i) |
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Where on the tRNA are the identifying elements for the aminoacyl-tRNA synthetase? |
In the anticodon loop, on the acceptor stem helix, or on the variable loop (depends on the specific synthetase) |
