Biochem Final

Front 1

true or false:
The ETC is highly exergonic?

Back 1

true

Front 2

what are the strong electron donors of the ETC?

Back 2

NADH and FADH2

Front 3

What is a strong electron acceptor in the TCA cycle?

Back 3

molecular O2

Front 4

Coupling of ETC to ATP synthase creates what?

Back 4

electrochemical gradient across the membrane

Front 5

what is the rate of respiration controlled by in the ETC?

Back 5

availability of ADP

Front 6

What is the function of the chemiosmotic hypothesis?

Back 6

ATP synthesis is driven by the electrochemical gradient.

Front 7

What are the two main components of ATP synthase?

Back 7

F0 and F1

Front 8

What is the function of F0?

Back 8

forms the transmembrane channel for the movement of H+ into the matrix

Front 9

What is the catalytic component of ATP synthase (the beta subunit)

Back 9

F1

Front 10

another proposed mechanism of the ETC is the exchange of energy via a high-energy______?

Back 10

phosphate which phosphorylates ADP

Front 11

the free energy of e transport is temporarily stored in a high-energy ______ of the carriers themselves.

Back 11

conformation

Front 12

what will stop ATP synthesis without affecting the electron transport?

Back 12

uncouplers of oxidative phosphorylation

Front 13

what happens to the free energy in the ETC?

Back 13

it is released as heat

Front 14

true or false:
carriers are bound to uncouplers?

Back 14

false

Front 15

The following things are characteristic of what molecule?
-an amphipathic molecule
-actually carries H+ across the membrane
-results in dissapation of the gradient

Back 15

2,4-Dinitrophenol

Front 16

true or false:
2,4-Dinitrophenol speeds up ATP?

Back 16

true
(Dr. Davidson said this during lecture)

Front 17

What creates a membrane channel in the ETC?

Back 17

Ionophores

Front 18

True or false:
Thermogenin is not a natural endogenous uncoupler in the inner mitochondrion matrix of mammals.

Back 18

false, it is

Front 19

True or false:
Thermogenin is a protein?

Back 19

true

Front 20

What is the function of respiratory inhibitors in the ETC?

Back 20

they block the flow of the elecrons

Front 21

What is the function of site-specific inhibitors?

Back 21

binding to a component in the chain

Front 22

true or false:
Inhibitors unblock the ox/red of the carrier?

Back 22

false, they block the carrier

Front 23

Complex I -> ?

Back 23

CoQ

Front 24

CoQ-?

Back 24

barbituates (amytal)
e.g. rotenone

Front 25

CoQ->

Back 25

cyt b- cyt c

Front 26

cyt b- cyt c -

Back 26

actimycin A

Front 27

Complex IV-

Back 27

binding of cyt a3

Front 28

cyt a3-

Back 28

CN-, H2S, CO, azide (N3-)

Front 29

which complex consists of serious inhibitors that will block the ETC?

Back 29

complex IV

Front 30

What are the 2 inhibitors of ATP synthase?

Back 30

Oligomycin and DCCD

Front 31

what is the mechanism of the inhibitors of ATP synthase

Back 31

bind to F0- blocks the H+ channel

Front 32

true or false:
Inhibitors of ATP synthase will directly affect the ETC?

Back 32

false, it will indirectly affect the ETC because no protons will be coming into the ETC, therefore, the chain would be too steep and would not be able to overcome its barriers.

Front 33

true or false:
Thermogenin is an uncoupler protein?

Back 33

true

Front 34

Atractyloside inhibits what?

Back 34

the ATP/ ADP translocase

Front 35

true or false:
In ATP/ ADP translocase, a single membrane translocator is involved?

Back 35

true

Front 36

true or false:
ATP/ADP translocase is in the inner mitochondrion matrix?

Back 36

true

Front 37

what is the consensus of H+ pumped in :
NADH -> O2- ?

Back 37

10 H+/ 2e's

Front 38

What would old textbooks say about the number of ATP synthesized for every 2 e's passed along the ETC?
(how many NADH; FADH2)

Back 38

3 NADH and 2 FADH2

Front 39

What would current textbooks say about the number of ATP synthesized for every 2 e's passed along the ETC?

Back 39

2.5 NADH and 1.5 FADH2

Front 40

Can NADH cross the inner mitochondrion matrix?

Back 40

no

Front 41

the _____-_____ shuttle transports NADH across the inner mitochondrion matrix?

Back 41

malate-aspartate

Front 42

the______ shuttle transports FADH2 across the inner mitochondrion matrix?

Back 42

glycerophosphate

Front 43

using the "new system", how many ATP's are produced; using two pyruvates?

Back 43

36

Front 44

using the "old system" how many ATP's are produced; using two pyruvates?

Back 44

38