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10 Cards in this Set
- Front
- Back
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What is a plasmid?
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A small circular piece of DNA that is independent of the chromosomal DNA.
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What is a "selectable marker"?
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A gene in a plasmid used to select for cells that contain the plasmid and cloned DNA.
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Why is glutathione called a cellular "antioxident"?
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Glutathione reacts with and reduces harmful oxidizing agents before they have a chance to damage cellular components.
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Why is heat-stable DNA polymerase used in PCR?
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PCR uses high temperature to denature the DNA template. Using heat stable polymerase prevents the enzyme from also denaturing and inactivating.
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A segment of DNA containing 20 base pairs includes 6 guanine residues. Therefore, there are ___ adenine residues and 0 uracil residues in this segment.
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14, 0
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If the DNA sequence read directly from a dideoxy sequencing gel is 5'-CCATCGTT-3' then the template sequence is 5'-________-3'
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AACGATGG
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In gel filtration chromatography molecules are separated on the basis of _______, with ________ molecules eluting before _______ molecules. In ion exchange chromatography, molecules are separated on the basis of ____________, and if the matrix contains DEAE groups, molecules with __________ will elute before molecules with ____________. In SDS-PAGE, molecules are separated on the basis of __________.
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size, large, small, charge, positive charge, negative charge, mass
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A genomic library is larger than a cDNA library for a given organism because _____________________________
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The genomic library contains all of the DNA as opposed to just the DNA transcribed to RNA.
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In two homologous proteins, a _________________ residue is most likely to replace a Glu residue as a conservative substitiution, and a ________________________ residue would replace a Trp residue in an invariant position.
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Asp, Trp
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Characteristics of double-helix DNA:
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Major and minor grooves,
strands are anti-parallel, bases are complementary, strands held together by H-bonds, right handed helix |
