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70 Cards in this Set
- Front
- Back
cardiac muscle is rich in _____ but have very little ______
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mitochondria; glycogen
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how does cardiac muscle mainly obtain energy?
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oxidative phosphorylation
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what are two main molecular components of muscle contraction?
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Myosin and actin
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what three molecular components of muscle contraction regulate muscle contraction?
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troponins, tropomyosin and calcium ion
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what are the three major causes of muscle diseases?
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insufficient energy supply, impaired neurological control, and loss of function mutations in structural proteins
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What provides energy for muscle contraction?
what are two possible causes of insufficient energy supply? |
ATP
ischemia and genetic diseases of fuel metabolism |
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what is a possible cause of impaired neurological control?
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myasthenia gravis
(not enough intracellular Ca2+) |
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what are two possible loss of function mutations in muscle structural proteins?
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muscular dystrophies and cardiomyopathies
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what are both duchenne and becker muscular dystrophies caused by?
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mutations in dystrophin genes
(result in patients wheel chair bound by age 13 or 16, Duschenne more severe) |
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what is dystrophin?
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molecule that participates in linking muscle cytoskeleton to membrane proteins in the sarcolemma
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what are the two major sources of ATP in muscle?
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glycolysis and oxidative phosphorylation
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what are the two sources of glucose for glycolysis?
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glycogen and blood glucose
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what are the four sources of NADH and FADH2 of oxidative phosphorylation?
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TCA cycle, beta oxidation of fatty acids, oxidation of branched-chain amino acids, and conversion of lactate to pyruvate
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what is the preferred source used for energy production depent on?
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muscle type and physiological conditions
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why is it not feasible to store ATP?
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ATP is an allosteric regulator of many enzymes
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in what form are high energy phosphate groups stored?
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creatine phosphate
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CPK/CK is a diagnostic tool for __________
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myocardial infarction
(muscle damage) |
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the synthesis of creatine involves two different tissues, what are they?
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liver and kidney
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the liver is the main metabolic site of what amino acid necessary for the synthesis of creatine?
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methionine (from S-A-M)
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in the synthesis of creatine, when glycine is converted to guanidinoacetate, what is arginine converted to?
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ornithine
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creatine phosphate decomposes through _________ _______ that produces creatinine
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spontaneous cyclization
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what does the daily urine creatinine excretion depend on?
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muscle mass
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what is the excretion of creatinine: fluctuating or constant?
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constant
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what is increase creatinine levels indicative of?
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impaired kidney function
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what does an increase in AMP upregulate?
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glycolysis, glycogenolysis and b-oxidation of fatty acids
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what enzyme converts ADP to ATP?
What is produced as a bi-product in this reaction? |
adenylate kinase
AMP |
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What would be the expected ADP/AMP ratio in the contracting muscle of an individual who has skeletal muscle adenylate kinase deficiency?
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The ratio would be higher than normal, because ADP is not being converted to ATP and AMP
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what induces phosphofructokinase 2 (PFK2) in the liver?
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glucagon and epinephrine
(opp of muscles) |
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in which of the three (liver, skeletal muscle and cardiac muscle) is glycolysis upregulated by PFK2?
in which is it downregulated? |
cardiac muscle
liver |
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what induced PFK2 in the heart?
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insulin, epinephrine, AMP levels
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which of the three (liver, cardiac muscle, skeletal muscle) has a non-phosphorylated PFK2? why?
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skeletal; because the enzyme that is phosphorylated in liver is absent in skeletal. In skeletal PFK2 is always on.
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Does PFK2 regulate glycolysis in skeletal muscle?
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no
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what inhibits carnitine palmitoyl transferase-1?
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malonyl CoA
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the level of malonyl CoA is dependent on the activities of what in reference to b-oxidation in muscle?
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(+) acetyl CoA carboxylase-2 (ACC-2)
(makes Malonyl Coa from acetyl CoA) and (-) malonyl CoA decarboxylase (MCoADC) (makes acetyl Coa from malonyl CoA) |
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what regulates ACC-2 and MCoADC?
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AMP, which activated AMP-PK
AMP-PK affects: (+) MCoADC (-) ACC-2 |
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High AMP level (AMP/ATP ratio) facilitates _____________, and leads to (activation/inhibition) of PFK1 and PFK2
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glucose uptake
activation of PFK1 and PFK2 |
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where does most of the energy come from in cardiac muscle under fasting blood glucose levels?
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long chain fatty acids
(FA degradation produces Acetyl-CoA, high Acetyl-CoA halts TCA and releases citrate substrate into cytosol) |
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high levels of acetyl CoA and citrate will limit energy production from glucose through glycolysis via what two enzymes?
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PFK-1 and PDH which are inhibited
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At high blood glucose levels (well-fed) insulin fascilitates ________ through Glut 4 and activates _____ which in turn will cause the activation of ______, increasing glycolysis.
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glucose uptake; PFK2; PFK1
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what does a lack of oxygen inhibit in cardiac muscle?
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mitochondrial ATP synthesis (TCA) and B oxidation of FAs
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why is pyruvate converted to lactate during ischemia in cardiac muscle?
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because oxidative phosphorylation cannot work without oxygen therefore inhibiting the TCA cycle
(acetyl-CoA is backed up from TCA and beta-ox. preventing pyruvate from becoming acetyl-coA) |
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the inhibition of oxidative phosphorylation leads to _______ in the cytosol
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acidosis
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where does all the energy for resting skeletal muscle come from?
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aerobic metabolism (oxidative phosphorylation)
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most of the energy in resting muscle is provided by what specific type of oxidative phosphorylation?
what can also be oxidized for energy? |
B-oxidation of fatty acids
branched chain AAs (if AA levels high) |
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If skeletal muscle has high energy during rest, high levels of citrate will reduce what two processes?
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glycolysis (inhibitiing PFK-1) and beta oxidation of fatty acids (activating ACC-2-->malonyl CoA)
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what is the preferred fuel in skeletal muscle during fasting?
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fatty acids
(less GLUT-4. less glucose can enter cell) |
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Fatty acids are the preferred fuel during fasting because:
low ATP levels leads to ___1___ AMP levels that activate ___2____. ___3_____ will deactivate ___4___ leading to low malonyl CoA. This then will activate CPTI and facilitate _____5___ of fatty acids producing large amounts of ____6___. This then inhibits __7____ and limits the use of glucose as energy source. |
1) high
2) AMP-PK 3) AMP-PK 4) ACC 5) b-oxidation 6) acetyl CoA 7) PDH |
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how long does it take for resting ATP levels to be used up?
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1.2 seconds at the start of exercise
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how long does it take for phosphocreatine to be used up if not regenerated?
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about 9 seconds
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Due to limited delivery of oxygen, how long after the onset of exercise is increased oxidative phosphorylation possible?
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1 min
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during the onset of exercise, what type of glycolysis can provide a quick ATP supply?
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anaerobic
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what two processes does anaerobic glycolysis activate?
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glycolysis and glycogenolysis
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why does increased intracellular Calcium during exercise also stimulate glycogen degradation?
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it binds to the allosteric activation of the calmodulin subunit of phosphorylase kinase
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which is a more sensitive indicator of energy need in exercising muscle AMP or ATP?
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AMP
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during high intensity exercise why is lactate produced?
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b oxidation and TCA cycle cannot produce enough ATP fast enough causing an accumulation of AMP which activates PFK-1 and glycolysis(anaerobic) creating pyruvate which is then converted to lactate.
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Why can't high intensity exercise be maintained for very long?
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1. depletion of muscle glycogen stores
2. lactic acidosis causes muscle pain and damage |
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lactate produced in exercising skeletal muscle can be used by what three parts of the body?
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resting skeletal muscle, cardiac muscle and liver
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what do resting skeletal muscle and cardiac muscle use lactate as?
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energy because they will convert lactate to pyruvate using lactate dehydrogenase. (if NADH is low)
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what does the liver use lactate for?
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converts lactate to pyruvate then glucose through gluconeogenesis. Then glucose can be release into circulation to be used for energy. (Cori cycle)
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what is the source of energy in long term exercise?
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aerobic oxidation of fatty acids and glucose
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up to 40 minutes of exercise, what is the main source of blood glucose?
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liver glycogen
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by 4 hours of exercise _______ increases and _______ decreases.
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gluconeogenesis; glucogenolysis
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why does overall glucose production decrease by 4 hrs of exercise?
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by that time muscle will increase its use of fatty acids as energy source
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what percentage of ATP can branched chain amino acids provide in resting skeletal muscle?
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20%
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(slow/fast) twitch muscle fibers have low glycogen content, high myoglobin content, high oxidative capacity, and high resistence to fatigue, in contrast to (slow/fast) twitch fibers.
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slow
fast |
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What changes in metabolite (ATP, creatine-phosphate, lactate) levels would be observed in biceps femoris after a 100 meter sprint?
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ATP-reduced
Creatine-phosphate- reduced Lactate- increased |
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When AMP levels rise during high intensity exercise, what cycle is accelerated?
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purine nucleotide cycle
(makes fumarate for TCA and ammonia for urine excretion of H+, decreasing acidosis) |
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What enzymatic modification is the most characteristic in the liver of someone who has been running 35 minutes?
Why? |
phosphorylation of glycogen phosphorylase
activated glycogen degradation in liver, main energy source up to 40 mins |
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How does acetate provide energy for skeletal muscle?
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It can be converted to acetyl CoA and used in TCA
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What is the main activator of glycolysis and glycogen degradation in exercising skeletal muscle?
What enzymes does it activate? |
AMP
glycolysis- activates PFK1 glycogen degradation- activates glycogen phosphorylase |