Bioch_Ch5B

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Book notes (p101):What protien characteristics are neccessary for Purification procedure?List and match

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Define electrophoresis?
-whats is it similar to?
-what does gell have?fnc?
-what moves slow or fast?

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PAGE?
-What is acrylamide?
-pore size?
-Seperation of protiens is based on what?
-Define native gel importance?
-what causes protien to migrate?

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SDS PAGE is alt form of what?
-how many SDS per how many aa?
-binds what and coats what with what kind of charge?
-What has more neg charge?
-Whats seperation based on?
-what moves farther?
-What helps you identify how big your protien is?

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Steps to ID protien of interest:
a) run what?
b) What gets transfered to what?
c) What gets passed through gel causes what?
d)What locates protien?Type of blot used? Draw chem rxn?

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Define BLOTS:
*western
*northern
*southern

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Isolectric Focusing
-uses what kind of gel?
-What is pI? charge?size?
-Protien sample in tube seperates according to?

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Ultracentrifugation:
-speed?
-based on what?
-define SVEDBERG?

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Preparative Ultracentrifug
-uses what to seperate by what?

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Zonal Ultracentrifugation:
-seperates according to what?
-makes what gradient that goes from what to what?

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Equilibrium density gradient ultracentrifug:
-seperates according to what?
-Dissolves what in what kind
of solution?
-How long do you spin it?
-When do protiens settleS?
-Equilibrium is what ...[?]

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List 3 ultracentrifugation techniques.

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What do you do once you have tube with purified protiens?

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Protien Sequencing:
-Define primary structure? how does it Relate to nucleic acid?
-Sanger developed what in 1953?

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1. What two things must be done to Determin # of subunits?
-What are subunits?
-define edman degradation

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2. What is cleaved with what kind of agent?
-name agent?
-draw bond? draw protien name type of group attached?
-What forms the covalent linkage?

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3. (optional) determin aa seq:
-what is actually det?
-name two steps

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4. Cleave what into shorter what?
-what is used to cleave it? How is this related to or diff from restruction enzymes/endocleases?

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5. Name this classic sequencing method? page 115
-How is the residue linked to two terminii?
-how many maxiumum residues before efficiency is reduced?

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6. Name this more modern technique?
-used for what kind of molecules?
-Determines what kind of ration?
-Solution is put through what?Spray goes through what? diagram this?
-Graph illustrates what?
-A mass analyzer is very what? small molecules vs large molecules...which bend fastest?
-Tech is accurate why?
-Calculates what of what?

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Tendem Mass strectrometry:
-Diagram
-What breaks pep bonds randomly?
-How does the graph tell you sequence?

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7) Reconstructive Polypeptide seq:
-form what?
-draw
-Give an ex of where protien sequencing is still done?

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What do you do with protien sequence?
1. Seq comparism
-is a clue to what
-similar what in diff what?

2. Protien family:
-simlar what in same what?
-often arise from what?
3. Protien Modules/domains:
-Setion of what with what two kinds of fncs?

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