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7 Cards in this Set
- Front
- Back
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What is transduction?
And what are the two types? |
Transfer of genetic info by a phage
Two types: Generalized -- may involve any host gene Specialized -- involves genes located near prophage |
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Describe the process of generalized transduction by Phage P1.
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Infection
Phage DNA replication Endonuclease activity, production of capsids and packaging enzymes Packaging DNA into phage particles Lysis |
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When complementing an auxotrophic mutant via transduction, how do you know that you aren't looking at original host carried over in your lysate?
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IDK...
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When complementing an auxotrophic mutant via transduction, how do you know that your mutant strain was really mutant or not contaminated with WT?
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IDK...
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When complementing an auxotrophic mutant via transduction, how do you know that the E. coli strain hasn't mutated (reversion) on its own to fix the Phe- phenotype?
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IDK...
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Where does the att region of phage DNA integrate?
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Between galactose and biotin operons
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Why might a prophage NOT be able to exit lysogeny and become lytic?
How can this be fixed? |
It is missing late genes (where are late genes? in the cos region?).
Can be rescued by super-infection with "helper phage" (wild type lambda) containing late structural genes. |
