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6 Cards in this Set
- Front
- Back
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Anabolic vs. Catabolic mutant???
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Help...
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What is the process to find a reversion mutation? (i.e. for Phe-)
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Start with Phe- auxotroph strain
Grow up large culture (include Phe) add mutagen/irradiate Plate ~10^7 or more cfu on MM (no phe) -- Only Phe+ should grow |
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What does the Ames Test do? How?
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Test for carninogens.
Have His- mutants, add a small amount of the chemical in question. If it is a mutagen then the number of colonies will increase in comparison to the control comparison plate with no add chemical (grown on liver extract -- chemicals are chemically modified in the liver, potentially causing them to become carcinogenic) |
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Base analogs usually cause what types of mutations?
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Point mutations (transversions/transitions)
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Alkylating agents usually cause what types of mutations?
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Deletions (resulting in frame shifts)
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Why is it important to use at least 2 strains when testing a suspected mutagen?
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A mutagen that causes deletions is UNlikely to revert a transition mutation. A mutagen that causes transition mutations is likely to revert a transition mutation, but UNlikely to revert a frameshift mutation
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