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25 Cards in this Set

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What does a typical photoautotroph need to function?

Light, DIC, DIN, DIP & DOP, metals, vitamins etc.

Diffusion is only useful when external [salt] > internal [salt], true or false?

True

In marine systems, is internal [salt] greater or less than external [salt]?

Greater than. Internal [salt] is measured in mm, whereas external [salt] is in nm or um.

What do marine organisms use to create low internal [Na+] and high internal [K+]?

Na/ K co-transporters and ATP

What comes in with Na+?

Nutrients

What kind of curve do Michaelis-Menten Kinetics produce?

Rectangular hyperbolic curve

Which is more beneficial, a low Kg and a high umax, or a high Kg and a low umax?

A low Kg and a high umax

What is Kt?

Substrate affinity

What is Tmax?

Maximum transport rate

Stress stimulates synthesis, therefore...

To alter Tmax, more porters are needed.

If DN is insufficient, what else can some cyanobacteria use?

N2

Examples of DIN?

Ammonium and nitrate

Which is more costly to use, NO3- or NH4+ and why?

NO3- because it is reduced internally to NH4+ at a 20 % cost to concurrent PS.

Examples of DON?

Urea, urease

What do cells need urease for?

Assimilation

How is synthesis controlled?

Repression

When is synthesis allowed?

When signals repressing are removed (de-repression)

What is metabolite repression?

Feedback control, typically from an end product

What is internal NH4+ assimilated into?

Glutamine

What are feedback controls for NH4+ and NO3- suggested as?

Glutamine, or 2-oxoglutaric acid (ketoglutaric acid)

What is an example of feedback control in the longer term?

Overall N-status (N:C)

What are the enzymes central to N-assimilation?

- Nitrate reductase (NR; NO3- to NO2-)


- Nitrite reductase (NiR; NO2- to NH4+)


- Glutamine synthetase (GS; Glu + NH4 to Gln)


- Glutamine, oxoglutaric acid transferase (GOGAT; Glu + 2OG to 2Glu)


- 2OG made from C fixation

Is NO2- toxic?

Yes

How is reductant made?

It is photogenerated using NADPH2

What is stress?

Caused by something that elicits an energy/resource costing response which is otherwise not required