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27 Cards in this Set
- Front
- Back
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Two culture media were inoculated with four different bacteria. After incubation, the following results were obtained: Red colonies in E. coli Colorless colonies in salmonella enterica no growth in S. aureus no growth in S. epidermidis
Medium 1 is a. selective. b. differential. c. both selective and differential. |
C |
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Medium 2 is a. selective. b. differential. c. both selective and differential. |
A |
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Log Phase (2) |
Cells will begin growth. They divide regularly, every generation is the same, until they run out of nutrients |
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Lag Phase (1) |
cells are getting ready to grow again. To grow fast. |
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Stationary Phase (3) |
The cells run out of nutrients. There is NO NET GROWTH/LOSS OF CELLS. |
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Death Phase (4) |
cells begin dying; # of cells you can require decrease over time |
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Assume you inoculated 100 facultatively anaerobic cells onto nutrient agar and incubated the plate aerobically. You then inoculated 100 cells of the same species onto nutrient agar and incubated the second plate anaerobically. After incubation for 24 hours, you should have a. more colonies on the aerobic plate. b. more colonies on the anaerobic plate. c. the same number of colonies on both plates. |
c. the same number of colonies on both plates. |
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The term trace elements refers to a. the elements CHONPS. b. vitamins. c. nitrogen, phosphorus, and sulfur. d. small mineral requirements. e. toxic substances. |
d. small mineral requirements. |
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Which one of the following temperatures would most likely kill a mesophile? a. -50°C b. 0°C c. 9°C d. 37°C e. 60°C |
e. 60°C |
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Which of the following is not a characteristic of biofilms? a. antibiotic resistance b. hydrogel c. iron deficiency d. quorum sensing |
c. iron deficiency |
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Which of the following types of media would not be used to culture aerobes? a. selective media b. reducing media c. enrichment media d. differential media e. complex media |
b. reducing media |
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An organism that has peroxidase and superoxide dismutase but lacks catalase is most likely an a. aerobe. b. aerotolerant anaerobe. c. obligate anaerobe. |
b. aerotolerant anaerobe |
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The optimum pH for most bacteria is a. 1 b. 5 c. 7 d. 9 |
c. 7 |
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Organisms that require high salt concentrations for growth are called a. Halophiles b. Extreme halophiles c. Facultative halophiles d. Microhalophiles |
b. Extreme halophiles |
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In food preservation, salts and sugars are used because they create a a. Lower pH b. Hypertonic environment c. Hypotonic environment d. Lower osmotic pressure |
b. Hypertonic Environment |
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Chemoautotrophs and photoautotrophs derive their carbon from a. Carbon dioxide b. Proteins c. Lipids d. Carbohydrates |
a. Carbon dioxide |
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An organism that uses oxygen but can grow without oxygen is called a(n) a. Obligate aerobe b. Facultative aerobe c. Aerobe d. Aerotolerant anaerobe |
b. Facultative aerobe |
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Biofilms reside in a matrix made up primarily of a. Lipids b. Hydrogel c. Proteins d. Polysaccharides |
d. Polysaccharides |
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Generation time can best be defined as a. The length of time it takes for lag phase b. The duration of log phase c. The length of time it takes for a cell to divide d. The minimum rate of doubling |
c. The length of time it takes for a cell to divide |
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Capnophiles must be grown in high concentrations of a. Oxygen b. Salt c. Glucose d. Carbon dioxide |
d. Carbon dioxide |
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The culture medium that is designed to suppress unwanted microbes while encouraging desired microbes is called a. Complex b. Selective c. Reducing d. Differential |
b. Selective |
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To neutralize acids as they are produced in a culture, what should be added to the culture medium? a. Buffers b. Salts c. Sugars d. PABA |
a. Buffers |
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What can be added to a medium when it is desirable to grow bacteria on a solid medium? a. Glucose b. Magnesium sulfate c. Potassium phosphate d. Agar |
d. Agar |
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The source of nutrients in nutrient agar is a. Agar b. Peptone and sodium chloride c. Peptone and beef extract d. Nutrient |
c. Peptone and beef extract |
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A spectrophotometer can be used to measure a. Metabolic activity b. Turbidity c. Dry weight d. Pathogenicity |
b. Turbidity |
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Which method is utilized to ensure that plates containing 25 to 250 colonies are counted? a. The most probable number method b. Serial dilution c. Filtration d. Spread plate method |
b. Serial dilution |
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Which of the following metabolic activities can be used to measure microbial growth? a. Carbon dioxide consumption b. Oxygen consumption c. Glucose consumption d. Salt consumption |
c. Glucose consumption |
