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75 Cards in this Set
- Front
- Back
Benificial Bacteria
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Lactobacillus- vigina and intestins
S. epidermidis - skin |
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Virulence vs Pahtogonicity
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Quantitative vs Qualitative ability to cause disease
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Virulence factors
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-antipagocytic capsule e.g. S. pneumonia
-exotoxin e.g. S. pyogenes -endotoxin (LPS) - Pilli adhesive e.g. Nesseria -adhesive capsules e.g. on teeth -enzymes, lecithinase in C. Perfringes |
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Lysoginistic conversion
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phagocyte puts DNA in Bacteria and it stays there for a few generations before making more virusis (Lysogeny) and the new DNA is expressed by the bacteria
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arthropod borne diseases
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carried by insects etc.
e.g. lyme disease, west nile, etc. |
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dysentary
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condition- abdominal pain
bloody diarrhea |
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nosocomial infections
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aquired during hospitalization
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Crown gall disease
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Agrobacterium tumefacieus - places T DNA from plasmid into plant cell DNA causeing growth and division making tumor
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Rizobia
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Fixes N2, 78% of environment, uses Nitrogenase, lugumes
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cyanobacterium
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can fix N2
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NO2-
NO3- |
Nitrite and Nitrate can be used by plants
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Cheese
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Milk + starter culture (L. cremoris or L. lactis) + rennet/rennin (enzyme) (ripened cheese only)
curds+ whey Press and mature hard-parmeasan, soft- brie additional cheese fermentation - Limburger- brevibacteria - Swiss- Propiombacterium |
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Yogurt
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starter culture - Streptococcus thermophilus
added- Lactobacillus bulgarious fermentation if sachromyses is also added you get kefir |
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preservation
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reduction in
pH water activity sugar concentration |
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probiotic
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bacteria added to food not needed for production but its good for you e.g. lactobacillus acidophilus, Bifidobacterium, or L. casei
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N2 in the atmosphere ->
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Organic nitrogen in plants through nitrogen fixation by Rhizobium or Anabaena
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Organic nitrogen in plants ->
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-Organic N in Consumers
-and then/or to NH3 or NH4+ through ammonification (decomposition) |
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NH3 ->
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NO2-, through nitrification by lithotrophs
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NO2-, ->
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NO3-, nitrification
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NO3-, ->
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uptaken by plants (back to start)
or it goes back to NO2- by pseudomonas, back to N2 by alcaligenes (Denitrification) |
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Nitrogen Cycle
Metabolism |
Anaerobic reperation: NO3- -> NO2- -> N2 (reductions) (Dentrification)
Chemoautotrophy: NH4+ -> NO2- (by Nitorsomonas) -> NO3- (by Nitrobacter) |
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S elemental sulfer ->
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SO4 2-, by sulfer oxidation, By photoautotrophic bacteria (Chromatium) or by chemoautotrophy (Thiobacillus)
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SO4 2- (sulfate)->
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uptake by plants then to H2S by decomposition
or Sulfate reduction to H2S by anaerobic resporation (e.g. Desulfovibrio) |
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H2S (sulfate) ->
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Elemental Sulfer S by
- spontaneous oxidation OR - H2S oxidation by photoautotrophy (Chramatium) or chemoautotrophic (Thiobacillus) |
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Fermented Vegtables
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Saurkraut - bacteria on Cabbage ferment + Leuconostoc plantarum
kimchi - asian cabbage pickles - fermented cucumber natto - soybeans |
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sourdough
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lactobaccilius and yeast
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Fermented Meats
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Starter culture = Pediococcus
Pepperoni salami country ham bologna |
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Pasture
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tried to figure out why wine -> vinegar
bacteria (acetobacter) pasturized grape juice (heated it) ad to add back yeast |
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MSG
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Monosodium glutamate, asian food, made by Corynebacterium glutamicum
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Xanthan gum
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bacterial slime, found in lots of stuff, creats texture, made by Xanthomonas campestris
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selective toxicity
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toxic to pathogens not to humans
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selective target
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organell or trait etc pathogen has that human cells dont. eg 70s ribosome
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Penecillin
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dystroys peptidoglycan bonds (beta-lactam)
gram + used for Staph, Strep, Nisseria Made from Penicillium |
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Nucleic acid synthesis
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no RNA polyerease in humans can be used as a selective target
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Efficiancy
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diameter / unit of anti-biotics
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bactericidal vs. bacteriostatic
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kills vs. stops growth
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Triclosan
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anti microbial
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TMV
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tobacco Mosaic virus
first virus discovered by Beijerinch it was filterable (went through filter) |
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1nm
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10^-9m
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shapes (7)
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cubic symmetry- icosahedral (20ish sides)
sperical - herpes helical- TMV Pleomorphic - influenza rhabdovirus (bullet shaped) - rabies complex - phage filamentous - ebola |
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structure
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single or double stranded DNA OR RNA IN A "CAPSID" (nucleocapsid)
spikes on outside of capsid OR on outside of membrane (envelope) outside of capsid. Capsomeres = single capsid proteins |
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Lysogenic cycle
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Phage DNA becomes part of Bacteria DNA and stays there for a few generations (some can do lytic and lysogenic called temperate phages e.g. Lambda phage, some can only do lytic called virulent phages e.g. T-even phage)
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Animal viruses
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1) recognition
2) attachment 3) penetration 4) uncoating (Nucleic acid out of capsid) 5) synthesis 6) assembly 7) release |
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receptor
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place were virus attaches, spikes fit into these receptors
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MIC vs. MBC
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Minimum inhibitory conentration - how much is needed to stop growth
minimum bactericidal concentration - how much is needed to kill bacteria |
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noninvasive
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not tending to spread
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nodule
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created by Rhizobium on plant roots so that they can fix N in not 21% O2 conditions
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CH4
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methane created by anaerobic digestion by methanobacterium/methanococcus
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secondary water treatment (4 options)
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Active sludge: aerated and bacteria are added to break down sewage
Trickling filter: sewege is "trickled" over rocks & gavel which become covered with a bio film of bacteria to break down sewage Lagoons: water is placed in artificial ponds bacteria breaks down sewage, cyanobacteria and algea e.g. spirulina make O2 Artificial wetlands: same as lagoons + an animal habitat |
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bioremediation
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use of bacteria and fungi to degrade or detoxify pollutants in a given environment
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Xenobiotics
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synthetic compounds to different from environment to be broken down quickly
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sulfer bacteria
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photoautotrophic bacteria that helps in H2S oxidation
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bioleaching
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metal -> soluble form by oxidation by bacteria
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symbiont
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organism that is part of a symbiosis
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Other fermented foods
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Black tea
coffee cocoa bean sour cream |
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antiseptic
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disinfectant (chemical used to destroy microbes and viruses) mild enough to be used on skin
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Types of disinfectant (3)
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Quaternary ammonium compound: disinfectant used on food preperation surfaces
Phenolic compounds: antimicrobial e.g. triclosan Halogens: disinfectant or antiseptic e.g. chlorine |
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Flemming
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Found penicillan, the first antibiotic
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Ehrlich
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discovered the first antimicrobial drug, salvarsan
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Narrow spectrumvs. broad spectrum
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Can be used for some bacteria Vs. can be used for many bacteria
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Sufa drugs
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structuraly similar to para-aminobenzoic acid (PABA) inhibits enzyme which creates folic acid biosynthesis in the cell. enzyme is not present in humans
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beta-latamase
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enzyme that creates resistance to beta-lactam drugs
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fluoroquinolone
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inhibits bacteria by atacking supercoil of the dna
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R-factor plasmids
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resistance DNA
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autoclave
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sterilizes stuff with pressurised steam, endospores also killed
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ciprofloxacin
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stops Bacterial DN replication by dystroying an important enzyme
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Lister
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discovered the idea of using antiseptics on wounds to stop infection
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Phages have that others dont
Phages in host |
O
I - Tail M - Tail fibers Prophage |
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viron
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a virus in its extracellular form
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induction
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virus DNA leaves host DNA during lysogenic cycle
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Positive and negitive strand virus
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viruses that use different DNA strands neg- strand used for rna transcription
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receptor-mediated endocytosis
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allows cell to bring in what has bound to receptor e.g. virus
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Stanley
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Christalized TMV
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Chlamydia
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bad bacterial STD
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Rickettsia
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Virus causing Rocky mountin spotted fever
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