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7 Cards in this Set
- Front
- Back
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Describe the Sanger-Coulson dideoxy method.
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1)Denature DNA duplex to get the template strand.
2)Add primer that is complementary to the 3' end of the template DNA 3)Add DNA Polymerase, dNTPs, ddNTPs (w/ colored dyes) 4)Run Gel electrophresis |
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What are Genomic libraries?
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the total DNA extraced from nuclei and contains all of the DNA sequences of the species.
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What are cDNA libraries?
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Produced from mRNA present in a particular cell type and thus corresponds to the genes that are active in that type of cell
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How do you identify the gene?
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Add transcription factors, presences of mRNA confirms presence of the gene.
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What is transcriptomics?
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The study of mRNA expression levels.
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How do you identify gene expression? Explain this process.
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DNA microarray
1)take two samples of cells growing in different mediums. 2)mRNA is extracted from each sample and converted to cDNA w/ dye 3)cDNA is added to gene chip cDNA that hybridize to the chip DNA cause that spot to grow. (Note could have lots of possible errors) |
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What is proteomics?
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the study of proteins, particularly their structure and formation.
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