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29 Cards in this Set
- Front
- Back
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DNA is present in
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The cell of call living organisms
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The first step in biotechnology is to
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extracting DNA
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Scientists must be able to separate DNA from unwanted substances so that...
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DNA does not denature OR breakup
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Each cell contains how many feet of DNA
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9 feet SON
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IN an average meal, you eat approximately how many cells
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55,000,000
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93,205 is an important number.
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93,205 is an important number.
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DNA is found where in all eukaryotic cells?
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Nucleus
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Most cells have the ____________ chromosome number
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DIPLOID 2N
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plants that contain several sets of chromosomes are called
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polyploid
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Strawberies are octaploid 8n
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OCTAPLOID 8n
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DNA is enclosed in a cell membrane made of
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phospholipids
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DNA is also coiled around the
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proteins
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Both the ______ and the _______ must be removed to see the DNA
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phospholipd layer and proteins
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To extract DNA, you must remove what four things
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Cell membrane, Cytoplasm, nuclear membrane and proteins
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Phospholipids DO NOT dissolve in
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Water
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Soap gathers inbetween oil and water capturing the oil in a bubble called
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micelle
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Using soap in the strawberry experiment is because it removes
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phospholipid membranes
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By adding _________ to the solution, it can punch holes in the nuclear and cell membrane
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Salt
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The soapy solution also removes what in the strawberry experanment?
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Proteins
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DNA is tightly coiled around proteins called
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histones
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What two things must be removed to see DNA?
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proteins and phospholipid layer
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what can remove proteins just like in meat tenerizer?
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enzymes
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The larger parts of the cell can be removed by filtering the _____ from the _____
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solidform the liquid
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You must _______ the DNA from the remained liquid you filtered
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Spooled
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DNA dissolves in water but not in
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alcohol
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adding what will cause the DNA to separate out from the liquid filtrate?
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COLD alcohol
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DNA will appear as the _______ once the alcohol is added
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White precipitate
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We hold the tube from the top bot the bottom so tht the DNA stands wont
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break apart due to the heat in your hands
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the steps in STRAWBERRY DNA extraction are...
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*Place in ziplock bag
*Crush berrys *Place soap in bag (to dissolve phospholipid membrane) *add salt to punch holes on the nuclear and membranes. (also removes proteins) *remove larger parts by filtering the solid from the liquid *Spool the DNA after adding COLD alcohol |
