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30 Cards in this Set

  • Front
  • Back
gel electrophoresis
seperation of charged particles in a gel matrix by an electric field
in a basic solution a carboxyl group will be charged
in basic solution an amino group will be charged
in an acidic solution a carboxyl group will be charged
in an acidic solution an amino group will be charged
Amino acids with a negative charge in basic conditions
Aspartic Acid (Asp)
Glutamic Acid (Glu)
Amino acids with a positive charge in acidic conditions
Arginine (Arg)
Lysine (Lys)
Histidine (His)
What causes sickle cell anemia?
A mutation in the b chain gene results in a single amino acid change in the b chains of the hemoglobin protein. Hemoglobin S crystallizes inside the red blood cells and leads to a distortion in the shape of the cell, creating a sickle or crescent shape.
how much agarose and buffer for hemoglobin experiment
what pH
30 mL
9.2 pH
voltage on hemo expt
100-150 V
dye for hemo expt
bromophenol blue
can all abnormal hemo mol. be diagnosed by electrophoresis?
no not all have replaced amino acids with chargable amino acids
amino acicds that are positively charged at low pH
Arginine (Arg)
Lysine (Lys)
Histidine (His)
negatively charged amino acids at high pH
Aspartic Acid (Asp)
Glutamic Acid (Glu)
how do you tally charge for a basic solution protein
-1 for the amino end grp
-1 for every asp, or glu
how do you tally charge on a protein for an acidic soln.
+1 for the carboxyl group
+1 for every arg, lys, or his
steps to do pcr from scratch
dna material into tube with MM 1 and incubate 1 hr at 65 deg
boiling bath for 10 minutes
take some liquid from MM1 to MM2
cycle 30 times (94,50,72)
add some of this to MM3 and incubate 1 hr
add dye and load.
What is RFLP
restriction fragment length a characteristic of DNA molecules (arising from their differing nucleotide sequences) by which they may be distinguished and as the laboratory technique which uses this characteristic to compare DNA molecules. The technique is utilized in genetic fingerprinting and paternity testing
in the PCR machine what are the temp cycles and what is happening
94 for 30s - break bonds
50 for 30s - anneal
72 for 1m - replication
PCR gel make-up
.5g agarose to 25 mL buffer
what is a DNA ladder
incremental lengths of dna in a solution used when running a gel to be able to determine lengths of unknown dna
What 2 primers are needed to do PCR
one for 5` and one for 3`
(not a palindrome)
Genetic marker
A genetic marker is a known DNA sequence that can be identified by a simple assay.
Pal I restriction enzyme
cuts GGCC to GG CC
PCR lab independent variable
genetic markers
PCR lab dependent variable
brain cancer
hemoglobin lab dependent variable
migration through the gel
hemoglobin lab independent variable
type of hemoglobin
Dye for respiration
Respiration and fermentation lab
what was the wavelength that we were checking % transmittance at
600 nm