Bio Lab Test 2

Front 1

gel electrophoresis

Back 1

seperation of charged particles in a gel matrix by an electric field

Front 2

in a basic solution a carboxyl group will be charged

Back 2

negative

Front 3

in basic solution an amino group will be charged

Back 3

neutral

Front 4

in an acidic solution a carboxyl group will be charged

Back 4

neutral

Front 5

in an acidic solution an amino group will be charged

Back 5

positive

Front 6

Amino acids with a negative charge in basic conditions

Back 6

Aspartic Acid (Asp)
Glutamic Acid (Glu)

Front 7

Amino acids with a positive charge in acidic conditions

Back 7

Arginine (Arg)
Lysine (Lys)
Histidine (His)

Front 8

What causes sickle cell anemia?

Back 8

A mutation in the b chain gene results in a single amino acid change in the b chains of the hemoglobin protein. Hemoglobin S crystallizes inside the red blood cells and leads to a distortion in the shape of the cell, creating a sickle or crescent shape.

Front 9

how much agarose and buffer for hemoglobin experiment
what pH

Back 9

360mg
30 mL
9.2 pH

Front 10

voltage on hemo expt

Back 10

100-150 V

Front 11

dye for hemo expt

Back 11

bromophenol blue

Front 12

can all abnormal hemo mol. be diagnosed by electrophoresis?

Back 12

no not all have replaced amino acids with chargable amino acids

Front 13

amino acicds that are positively charged at low pH

Back 13

Arginine (Arg)
Lysine (Lys)
Histidine (His)

Front 14

negatively charged amino acids at high pH

Back 14

Aspartic Acid (Asp)
Glutamic Acid (Glu)

Front 15

how do you tally charge for a basic solution protein

Back 15

-1 for the amino end grp
-1 for every asp, or glu

Front 16

how do you tally charge on a protein for an acidic soln.

Back 16

+1 for the carboxyl group
+1 for every arg, lys, or his

Front 17

steps to do pcr from scratch

Back 17

dna material into tube with MM 1 and incubate 1 hr at 65 deg
boiling bath for 10 minutes
take some liquid from MM1 to MM2
cycle 30 times (94,50,72)
add some of this to MM3 and incubate 1 hr
add dye and load.

Front 18

What is RFLP

Back 18

restriction fragment length polymorphism.as a characteristic of DNA molecules (arising from their differing nucleotide sequences) by which they may be distinguished and as the laboratory technique which uses this characteristic to compare DNA molecules. The technique is utilized in genetic fingerprinting and paternity testing

Front 19

in the PCR machine what are the temp cycles and what is happening

Back 19

94 for 30s - break bonds
50 for 30s - anneal
72 for 1m - replication

Front 20

PCR gel make-up

Back 20

.5g agarose to 25 mL buffer

Front 21

what is a DNA ladder

Back 21

incremental lengths of dna in a solution used when running a gel to be able to determine lengths of unknown dna

Front 22

What 2 primers are needed to do PCR

Back 22

one for 5` and one for 3`
(not a palindrome)

Front 23

Genetic marker

Back 23

A genetic marker is a known DNA sequence that can be identified by a simple assay.

Front 24

Pal I restriction enzyme

Back 24

cuts GGCC to GG CC

Front 25

PCR lab independent variable

Back 25

genetic markers

Front 26

PCR lab dependent variable

Back 26

brain cancer

Front 27

hemoglobin lab dependent variable

Back 27

migration through the gel

Front 28

hemoglobin lab independent variable

Back 28

type of hemoglobin

Front 29

Dye for respiration

Back 29

DPIP

Front 30

Respiration and fermentation lab
what was the wavelength that we were checking % transmittance at

Back 30

600 nm