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38 Cards in this Set
- Front
- Back
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What do you do if the field of view is not illuminated?
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Turn the light on. Check/Open the iris diaphragm. Ensure objective lens is in place. Adjust ocular lens.
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What if you cannot find a specimen on the slide?
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Goto low power and recenter the slide. Focus on the edge of the coverslip. Check position of the condenser
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What microscopes make an image appear inverted?
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Compound
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1m = ___ cm
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100cm
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100cm = __mm
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1000mm
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1mm = ___micrometer
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1000micrometers
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1micrometer = ___nm
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1000nanomaters
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What is the total Dissecting Microscope magnification on low and high power?
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Low: Ocular- 10X / Objective 2X= 20X.
High: Ocular 10X / Objective 4X = 40X |
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Compound Microscope Magnifying Power is?
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Low: Ocular 10X/ Objective 4X - Total 40X
Medium- 10X / Objective 20X = 100X High Ocular 10X/ Objective 40X = 400X |
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What is the calculation for Size of a Specimen?
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FractionOfField X Field Diamter
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Speed of Specimen Calculation
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Speed = Distance / Time
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Magnification of Drawing Calculation
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Magnification = Size of Drawing / Actual Size of specimen
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Size of drawing Calculation
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Size of Drawing = Magnification X Actual Size
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Actual Size Calculation
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ActualSize= Size of Drawing/Magnification
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What is the relationship between magnification and depth of field
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The higher the magnification the lower the depth of field
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What happens to the resolution and resolving power when you decrease wavelength of light?
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Smaller resolving power, better resolution
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How much greater is the electron microscopes resolving power than the eye?
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500,000X's
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How do you improve the contrast of a specimen?
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Stain it with artificial dyes. Use a special light microscope. Reduce the light intensity
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What is the smallest Carbohydrate?
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Monosaccharides
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Disaccharides
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Two sugars
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Oligosaccharides
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Several sugars
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Polysaccharides
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Thousands of sugars
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Give two examples of Polysaccharides
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Starch, Glycogen, Cellulose
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Glucose is what type of Saccharide?
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Mono
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Sucrose is what type of saccharide?
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Disaccharide
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Starch is a ____ consisting of many ____ monomers
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Polysaccharide, glucose
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Lipids
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Diverse group that are characterized by low or no solubility in water
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What do fats and oils consist of?
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Fatty acid tails
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Saturated Fats
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If the tails carry the maximum number of hydrogen atoms bonded to the carbon backbone
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Unsaturated Fats
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If the tails have one or more double bonded carbon
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Proteins
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Long chains made up of monomers called amino acids
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Proteins are always joined together by a bond called
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Peptide Linkage
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Nucleic Acids
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Large molecules made of monomers called nucleotides
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Iodine Test
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Turns blue-black when met with Starch
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Benedict Tests
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Tests for reducing sugars. If a reducing sugar is heated in the presence of BS it gives up an electron to a Cu2+ ion turning Cu+, changing the color of the solution.
Positive tests yield: green (weak), yellow (stronger), orange, brown, or red (strongest) precipitate |
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Glucose and Gelatin when in contact with the benedicts test
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Weak positive.
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Sudan IV
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Tests for lipids. Turns pink or red when it contacts oil.
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Bromthymol Blue
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Used to monitor pH levels. Blue - Basic, Green - Neutral, Yellow- Acidic
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