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11 Cards in this Set

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Microscope

An instrument that magnifies specimen.

Specimen

Any object under observation.



ie: cells, tissue, urine, saliva



Types of Microscopes

1. Light Microscope


-max magnification 1000x


-uses both natural and artificial light


-observation of living and dead specimen




2. Electron Microscope


-uses electron beam


- observation of dead specimen only


- 2 types: TEM & SEM

TEM

TEM = Transmission Electron Microscope


-uranium (radioactive)


-used for research


-max magnification 1.5 million x

SEM

SEM = Scanning Electron Microscope


-cell structure in 3D


-research = gold vapor


-max magnification 500,000x

Ocular/Eyepiece

Point of observation.


Magnifies 10x

Stage

Point of mounting specimen

Objective Lenses

Provides additional magnification.




Red = 4x


Yellow = 10x


Blue = 40x

Coarse & Fine Adjustment Knobs

Focus Specimen

Condenser/Iris Diaphragm


Regulation of light intensity

Computation of total magnification

Formula:




Total mag = Eyepiece mag x Objective lens mag




40x = (10x) x (4x)


100x = (10x) x (10x)


400x = (10x) x (40x)