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10 Cards in this Set
- Front
- Back
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Gel Electrophoresis
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Seperates DNA restriction fragments of different lengths.
Used for sequencing. |
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Southern Blotting
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Compares restriction fragments produced from different samples of DNA
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PCR (Polymerase Chain Reaction)
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A quick and selective way to make copies of a specific gene or DNA Sequence.
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YACs (Yeast Artificial Chromosomes)
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Combine and origin for replication, a centromere, and two telomeres - with foreign DNA
Clones Eukaryotic Genes |
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Electroporation
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brief electrical pulse to create temporary holes in plasma membrane for DNA to enter
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RFLPs (Restriction Fragment Length Polymorphisms)
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Detected by southern blotting
Differences on DNA sequence of homologous chromosomes resulting in different pattern lengths |
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BAC (Bacterial Artificial Chromosome)
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artificial version of a bacterial chromosome containing hundreds of thousands of base pairs
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Dideoxy Chain-Termination Method
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Sequences DNA
Seperates products by length and sequences nucleotides in cloned DNA (MACHINERY) |
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In-vitro mutagenisis
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takes out a sequence of DNA and replaces it with a mutagen to determine the function of the missing DNA
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DNA Microarray Assay
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tiny amounts of single stranded DNA on a grid
Determines which DNA sequences are being expressed |
