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13 Cards in this Set
- Front
- Back
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What is Colchisine used for?
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Inhibits tubulin (microtubule) polymerization
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What is BDM used for?
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myosin inhibitor, but also general ATPase inhibitor.
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What is cytochalasin B used for?
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Prevents actin polymerization.
Blocks formation of microfilaments. |
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What is Phalloidin used for?
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Visualizes actin filaments (when fluorescently labelled).
Also prevents actin filaments from breaking down. |
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What is Northern blot used for?
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Used to detect targetted RNA sequences; Can give limited quantitative information.
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What is Southern blot used for?
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Used to detect particular DNA sequences.
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What is Western blot used for?
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For detecting proteins.
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What is tritiated-water release assay used for?
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For measuring enzyme activity.
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What is relative luciferase activity used for?
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Measures how much protein a particular gene is making.
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What is ChIP used for?
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Use to detect what part of DNA a protein is binding to.
(ChIP was used to confirm SF-1 was an aromatase transcription factor.) |
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What is ferlin?
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A membrane protein, expressed in testes and ovaries, that causes infertility when mutated in male Drosophilia.
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What is RT-PCR used for?
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To quantify the amount of RNA transcripts.
( Technically RT-PCR quantifies DNA, but mRNA transcripts are usually converted to cDNA first and then tested. ) |
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What is the MTT assay?
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Measures the number of viable cells.
This assay can tell you if your culture is growing or dying over time. |
