Bio 325 Lec2-8

Front 1

Egg cylinder

Back 1

An asymmetric structure that helps to determine the body plan for the mouse

Front 2

Primitive endoderm & Visceral endoderm

Back 2

also known as the as the hypoblast, responisible for creating extra-embryonic ectoderm and WNT signaling to epiblast

Front 3

Extra-embryonic ectoderm

Back 3

created by primitive Endoderm, considered yolk of the egg

Front 4

Embryonic ectoderm/epiblast

Back 4

tissue that all cells body arise from

Front 5

Primitive streak

Back 5

created by epiblast and gives rise to embryonic endoderm and mesoderm

Front 6

Blimp (Prdm1)

Back 6

gene activated by BMP in PGCs, keeps them pluripotent, stops apoptosis

Front 7

Prdm14

Back 7

gene activated by BMP in PGCs, keeps them pluripotent and silences chromatin

Front 8

Apoptosis

Back 8

programmed cell death

Front 9

Alkaline phosphatase

Back 9

enzyme that helps to track PGCs

Front 10

Egg cylinder

Back 10

An asymmetric structure that helps to determine the body plan for the mouse

Front 11

Stem Cell Factor

Back 11

helps to move PGCs

Front 12

Primitive endoderm & Visceral endoderm

Back 12

also known as the as the hypoblast, responisible for creating extra-embryonic ectoderm and WNT signaling to epiblast

Front 13

Extra-embryonic ectoderm

Back 13

created by primitive Endoderm, considered yolk of the egg

Front 14

Embryonic ectoderm/epiblast

Back 14

tissue that all cells body arise from

Front 15

Primitive streak

Back 15

created by epiblast and gives rise to embryonic endoderm and mesoderm

Front 16

Blimp (Prdm1)

Back 16

gene activated by BMP in PGCs, keeps them pluripotent, stops apoptosis

Front 17

Prdm14

Back 17

gene activated by BMP in PGCs, keeps them pluripotent and silences chromatin

Front 18

Apoptosis

Back 18

programmed cell death

Front 19

Alkaline phosphatase

Back 19

enzyme that helps to track PGCs

Front 20

Stem Cell Factor

Back 20

helps to move PGCs

Front 21

Egg cylinder

Back 21

An asymmetric structure that helps to determine the body plan for the mouse

Front 22

Primitive endoderm & Visceral endoderm

Back 22

also known as the as the hypoblast, responisible for creating extra-embryonic ectoderm and WNT signaling to epiblast

Front 23

Extra-embryonic ectoderm

Back 23

created by primitive Endoderm, considered yolk of the egg

Front 24

Embryonic ectoderm/epiblast

Back 24

tissue that all cells body arise from

Front 25

Primitive streak

Back 25

created by epiblast and gives rise to embryonic endoderm and mesoderm

Front 26

Blimp (Prdm1)

Back 26

gene activated by BMP in PGCs, keeps them pluripotent, stops apoptosis

Front 27

Prdm14

Back 27

gene activated by BMP in PGCs, keeps them pluripotent and silences chromatin

Front 28

Apoptosis

Back 28

programmed cell death

Front 29

Alkaline phosphatase

Back 29

enzyme that helps to track PGCs

Front 30

Stem Cell Factor

Back 30

helps to move PGCs

Front 31

Hindgut

Back 31

area behind the gut where the PGCs migrate too

Front 32

Embryonic Stem Cell

Back 32

Pluripotent Stem Cells that are removed from inner cell mass that are cultured in a way to remain pluripotent

Front 33

Venus

Back 33

flourescent gene

Front 34

How do correlative and Functional evidence differ?

Back 34

functional evidence is a when you remove something and event doesn't happen or when you add something and the event occurs, loss of function and gain of function respectively where as correlative evidence is when there is a link between to events and you infer that one event brings about the other

Front 35

Hindgut

Back 35

area behind the gut where the PGCs migrate too

Front 36

2. Where do primordial germ cells originate?

Back 36

From the epiblast

Front 37

Embryonic Stem Cell

Back 37

Pluripotent Stem Cells that are removed from inner cell mass that are cultured in a way to remain pluripotent

Front 38

3. What signal(s) are required for specification of primordial germ cells?

Back 38

WNT and BMP

Front 39

Hindgut

Back 39

area behind the gut where the PGCs migrate too

Front 40

Venus

Back 40

flourescent gene

Front 41

Embryonic Stem Cell

Back 41

Pluripotent Stem Cells that are removed from inner cell mass that are cultured in a way to remain pluripotent

Front 42

4. Which tissues produce these signals? Which tissues respond to the signal(s)?

Back 42

Extraembryonic cells produce BMP signal while Visceral Endoderm produces WNT signal, epiblast responds to these signals. WNT allows the epiblast cells competence to respond to BMP

Front 43

How do correlative and Functional evidence differ?

Back 43

functional evidence is a when you remove something and event doesn't happen or when you add something and the event occurs, loss of function and gain of function respectively where as correlative evidence is when there is a link between to events and you infer that one event brings about the other

Front 44

Venus

Back 44

flourescent gene

Front 45

5. Which genes are activated in the primordial germ cells in response to BMP signals?

Back 45

blimp1(Prdm1) Prdm 14

Front 46

2. Where do primordial germ cells originate?

Back 46

From the epiblast

Front 47

How do correlative and Functional evidence differ?

Back 47

functional evidence is a when you remove something and event doesn't happen or when you add something and the event occurs, loss of function and gain of function respectively where as correlative evidence is when there is a link between to events and you infer that one event brings about the other

Front 48

3. What signal(s) are required for specification of primordial germ cells?

Back 48

WNT and BMP

Front 49

6. How do Blimp1 (Prdm1) and Prdm14 regulate primordial germ cell fate determination?

Back 49

blimp1 keeps cells pluripotent with sox2 and nanog, stops apoptosis with Nanos 3, prdm14 keeps pluripotency by chromatin silencing sox 2

Front 50

7. How do primordial germ cells differ from somatic mesoderm cells?

Back 50

Somatic mesoderm cells are multipotent while germ cells are pluripotent

Front 51

2. Where do primordial germ cells originate?

Back 51

From the epiblast

Front 52

4. Which tissues produce these signals? Which tissues respond to the signal(s)?

Back 52

Extraembryonic cells produce BMP signal while Visceral Endoderm produces WNT signal, epiblast responds to these signals. WNT allows the epiblast cells competence to respond to BMP

Front 53

5. Which genes are activated in the primordial germ cells in response to BMP signals?

Back 53

blimp1(Prdm1) Prdm 14

Front 54

3. What signal(s) are required for specification of primordial germ cells?

Back 54

WNT and BMP

Front 55

4. Which tissues produce these signals? Which tissues respond to the signal(s)?

Back 55

Extraembryonic cells produce BMP signal while Visceral Endoderm produces WNT signal, epiblast responds to these signals. WNT allows the epiblast cells competence to respond to BMP

Front 56

6. How do Blimp1 (Prdm1) and Prdm14 regulate primordial germ cell fate determination?

Back 56

blimp1 keeps cells pluripotent with sox2 and nanog, stops apoptosis with Nanos 3, prdm14 keeps pluripotency by chromatin silencing sox 2

Front 57

7. How do primordial germ cells differ from somatic mesoderm cells?

Back 57

Somatic mesoderm cells are multipotent while germ cells are pluripotent

Front 58

5. Which genes are activated in the primordial germ cells in response to BMP signals?

Back 58

blimp1(Prdm1) Prdm 14

Front 59

6. How do Blimp1 (Prdm1) and Prdm14 regulate primordial germ cell fate determination?

Back 59

blimp1 keeps cells pluripotent with sox2 and nanog, stops apoptosis with Nanos 3, prdm14 keeps pluripotency by chromatin silencing sox 2

Front 60

7. How do primordial germ cells differ from somatic mesoderm cells?

Back 60

Somatic mesoderm cells are multipotent while germ cells are pluripotent