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39 Cards in this Set

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(T/F) Archaea do not look like bacteria

false

how did scientists find the difference between archaea and bacteria? year?

1970s and because of the ribosomal RNA`

what is an extremeophile?

archaea: represent 20% of microbial cells in the oceans, live in high temp/low temp, high/low pH, also they are present in normal habitats among the plankton community

what are methanogens?

first "archaeons" poorly characterized as microbes producing methane gas

what is the habitat for halobacterium salinarium?

dead sea, salted foods

what is the habitat for Pyrococcus furiosus?

hydrothermal vents; used as DNA polymerase is PCR (can be used at high temps)

what is the habitat for Picrophilus oshimae?

sulfur rich volcanic regions

what is the habitat for methanogenium frigidum?

ace lake, antartica

(T/F) the size and shape of bacteria and archaea are very similar

true

what is the size of archaea?

0.5-5 micrometers, but it can vary greatly

do archaea have membrane-bound nucleus?

no

(T/F) Archaea have unique plasma membrane.

true

what is a histone?

they are structures that control the expression of genes and they are made up of proteins/carbs/lipids. Positively charged, pack the DNA into one area.

(T/F) the shapes of bacteria and archaea are different.

false; all of the shapes that bacteria have, archaea also have them

(T/F) Ignicoccus and nanoachaeum are never found next to eachtoher.

false; they are always found next to each other but the reason for this is unknown

can both the size and shape of archaea vary?

yes; archaea have rods, spheres, spirals, irregular, and rectangular

what is the purpose of the rectangular shaped archaea?

it is unknown, unique to archaea

how many histones are present in archaea/bacteria?

only 4 in archaea and 8 in bacteria

(T/F) histones are exactly the same in archaea and eukarya.

false; the structure and wrapping are very different

which proteins are present in archaea cytoskeleton?

Ta0583; these are homologous to actin

(T/F) All archaeons have a PM and CW.

false; they all have a PM but not all have CW

What is a monolayer?

they are for extremely high temperatures, and each lipid has a phosphoglycerol molecule on both ends; unique to archaea

what is an isoprenoids?

for archaea; they can be both monolayer or bilayer and they do not have fatty acids. They have ether linkages and it is a single layer when two glycerol molecules are connected to each other

(T/F) Ignicoccus are similar to gram positive cells.

false; they are similar to gram negative cells in that they have an outer membrane and periplasm

what is the CW composed of in archaea?

pseudomurein; NAG & NAT, peptide links to NAT, and it is an Lamino acid

how are archaeon flagella different from bacterial one?

they are thinner, different flagellin proteins, they grow from the base rather than the tip, and they are solid rather than hollow

Where was the genetic code discovered?

E. coli

Do archaea have pathogens?

no

how long have we been studying archaea?

about 20 years

What are crenarchaeota?

thermophiles, hyperthermophiles, acidophiles, barophiles

What adaptations do crenarchaeota have? (5 answers)

tetraether/monolayer lipids, more salt bridges/sidechain interactions in proteins, strong chaperone proteins, thermostable DNA-binding proteins, reverse DNA gyrase enzyme to increase DNA supercoiling

What are mesophiles?

crenarchaeota that are detected by rRNA sequences and survive in 15-40C

What are psychrophiles?

crenarchaeota that are detected by rRNA sequences and survive in <15C

What are methanogens?

euryarchaeota; methane is odorless

What are halophiles?

euryarchaeota; require NaCl concentration greater than 1.5M, like high salt environments (dead sea and great salt lake in Utah), they are phototrophes

how do halophiles deal with osmotic shock and loss of water?

they have evolved a system to the point that the water is not hypertonic to them and they have a lot of potassium inside them

how do halophiles deal with the adaptation to salty environments?

higher GC content to prevent DNA denaturing and highly acidic proteins remain stable in these environments which prevents protein denaturing

how do halophiles produce energy?

use bacteriorhodopsin to harness light energy and produce proton motive force to make ATP

What is a nanoarchaeota?

new phylum for archaea